Biosorption of Ni(II) Using Seeds of Mirabilis Jalapa
57
graphs. FTIR analysis was performed for samples obtained before and after nickel
adsorption. The spectral range taken for our sample is 4000–1000 cm
−1 (Aravind
et al. 2015).
2.3 Metal Analysis
2.3.1 Preparation of Metal Solution
A stock solution of Ni(II) ions were prepared for 1000 ppm using NiCl 2 , the stock
was diluted to 100 ppm which is used for further studies. The pH of the solution was
adjusted using 0.1 N HCl or NaOH solutions.
2.3.2 Nickel Analysis
DMG (dimethylglyoxime) method was used for Nickel(II) spectrophotometric analysis. A blank was prepared for Ni(II) solution. To different standard concentrations
of Ni(II), 2 mL of 20% (w/v) potassium sodium tartrate solution, 10 mL of 4% (w/v)
ammonium per sulfate, 2.5 mL of 5 M sodium hydroxide solutions and 0.5 mL of 1%
DMG (w/v) solutions were sequentially added. After an incubation period 45 mins,
spectrometric measurement was performed at 450 nm. The amount of nickel present
in the sample was determined using the calibration curve (Aravind et al. 2015).
2.4 Removal Efficiency
The nickel removal percentage can be obtained using the formulae:
Removal efficiency (%) = (Ci − Co/Ci) × 100
(1)
where Ci represents the initial metal ion concentration and Co represents the final
metal ion concentration.
2.5 Batch Adsorption Studies
Adsorption studies were performed initially by taking the appropriate amount of
stock solution dissolved in water to obtain desired dilutions in a conical flask. They
were subjected to initial studies by maintaining a certain pH, rpm for a certain period.
Then the samples were filtered, and the solution was assayed using DMG method
and analyzed spectrophometrically at 450 nm.
57
graphs. FTIR analysis was performed for samples obtained before and after nickel
adsorption. The spectral range taken for our sample is 4000–1000 cm
−1 (Aravind
et al. 2015).
2.3 Metal Analysis
2.3.1 Preparation of Metal Solution
A stock solution of Ni(II) ions were prepared for 1000 ppm using NiCl 2 , the stock
was diluted to 100 ppm which is used for further studies. The pH of the solution was
adjusted using 0.1 N HCl or NaOH solutions.
2.3.2 Nickel Analysis
DMG (dimethylglyoxime) method was used for Nickel(II) spectrophotometric analysis. A blank was prepared for Ni(II) solution. To different standard concentrations
of Ni(II), 2 mL of 20% (w/v) potassium sodium tartrate solution, 10 mL of 4% (w/v)
ammonium per sulfate, 2.5 mL of 5 M sodium hydroxide solutions and 0.5 mL of 1%
DMG (w/v) solutions were sequentially added. After an incubation period 45 mins,
spectrometric measurement was performed at 450 nm. The amount of nickel present
in the sample was determined using the calibration curve (Aravind et al. 2015).
2.4 Removal Efficiency
The nickel removal percentage can be obtained using the formulae:
Removal efficiency (%) = (Ci − Co/Ci) × 100
(1)
where Ci represents the initial metal ion concentration and Co represents the final
metal ion concentration.
2.5 Batch Adsorption Studies
Adsorption studies were performed initially by taking the appropriate amount of
stock solution dissolved in water to obtain desired dilutions in a conical flask. They
were subjected to initial studies by maintaining a certain pH, rpm for a certain period.
Then the samples were filtered, and the solution was assayed using DMG method
and analyzed spectrophometrically at 450 nm.