Screening, Isolation and Molecular Characterization …
251
(b)
(a)
Fig. 4 a Mass production in mineral salt medium b Emulsification activity: Serratia marcescens
Fig. 5 a Extraction of
biosurfactants with the
solvents chloroform and
methanol, b Dried
biosurfactants of Serratia
marcescens
(a)
(b)
3.6 16S RRNA Gene Sequencing
The DNA isolated from the bacterial culture was used in PCR to amplify the bacterial
16 s rRNA. The molecular analysis for the isolated bacterial strain showed 99%
identity to the sequence of Serratia marcescens. The phylogenetic tree generated for
the isolated bacteria shown in Fig. 5. and 99% identity to the sequence of Serratia
marcescens. Based upon the sequence analyses, the isolate is likely to be Serratia
marcescens. The phylogenetic tree generated for the isolated bacteria provided in
Fig. 6.
3.6.1 Thin Layer Chromatography
The retention factor for the compounds separated, calculated using the Formula (3),
251
(b)
(a)
Fig. 4 a Mass production in mineral salt medium b Emulsification activity: Serratia marcescens
Fig. 5 a Extraction of
biosurfactants with the
solvents chloroform and
methanol, b Dried
biosurfactants of Serratia
marcescens
(a)
(b)
3.6 16S RRNA Gene Sequencing
The DNA isolated from the bacterial culture was used in PCR to amplify the bacterial
16 s rRNA. The molecular analysis for the isolated bacterial strain showed 99%
identity to the sequence of Serratia marcescens. The phylogenetic tree generated for
the isolated bacteria shown in Fig. 5. and 99% identity to the sequence of Serratia
marcescens. Based upon the sequence analyses, the isolate is likely to be Serratia
marcescens. The phylogenetic tree generated for the isolated bacteria provided in
Fig. 6.
3.6.1 Thin Layer Chromatography
The retention factor for the compounds separated, calculated using the Formula (3),