238
S. Swathi Priya et al.
spectrophotometer and quantitative analysis on Agarose gel. Finally, sequencing was
obtained with the aid of the ABI sequencing machine (Babalola 2003).
3 Result and Discussion
The result of the estimation of microbial population size in specific dilution was
estimated (Fig. 1). The obtained microbes further confirmed (Figs. 2, 3, 4, 5 and
6). The results obtained from various medium of isolated food-borne pathogens
shown in the Table 1. The presence of microorganisms like Bacillus and Salmonella
proved via Mannitol salt agar and SS Agar respectively, while comparing the different
media for the growth of bacteria; MacConkey Agar and Triple Sugar Iron Agar.
Very less bacterial growth observed in SS Agar than Mannitol Salt Agar, hence,
this was considered for further processing. In the food sample, Bacillus sp., were
identified (asides some gram-negative bacteria) B. anthracis and B. cereus causes
food poisoning. Bacillus sp., is present widely in nature and food (Privitera and
Nesci 2015).
Fig. 2 Estimation of
microbial population size via
serial dilution method
Fig. 3 Mac Conkey Agar
S. Swathi Priya et al.
spectrophotometer and quantitative analysis on Agarose gel. Finally, sequencing was
obtained with the aid of the ABI sequencing machine (Babalola 2003).
3 Result and Discussion
The result of the estimation of microbial population size in specific dilution was
estimated (Fig. 1). The obtained microbes further confirmed (Figs. 2, 3, 4, 5 and
6). The results obtained from various medium of isolated food-borne pathogens
shown in the Table 1. The presence of microorganisms like Bacillus and Salmonella
proved via Mannitol salt agar and SS Agar respectively, while comparing the different
media for the growth of bacteria; MacConkey Agar and Triple Sugar Iron Agar.
Very less bacterial growth observed in SS Agar than Mannitol Salt Agar, hence,
this was considered for further processing. In the food sample, Bacillus sp., were
identified (asides some gram-negative bacteria) B. anthracis and B. cereus causes
food poisoning. Bacillus sp., is present widely in nature and food (Privitera and
Nesci 2015).
Fig. 2 Estimation of
microbial population size via
serial dilution method
Fig. 3 Mac Conkey Agar