Potential Utilization of a Weed Prosopis Juliflora Leaf Extract …
157
where Ci represents the initial dye concentration and Co represents the final dyeion
concentration.
2.4 Anti-bacterial Activity
The Acetone, Ethanol and Distilled water extracts of Prosopis juliflora, were analyzed
for their anti-bacterial activity for Pseudomonas putida, Bacillus subtilis (Ahmad
et al. 1988; Jeevan et al. 2012).
2.5 Fourier Transform Infra-Red Analysis (FTIR)
Fourier Transform-Infrared Spectrophotometer (FTIR) is a tool to identify types of
chemical compounds in the sample. Dried leaf powder (ethanolic extract) of test
plant was used for FTIR analysis. 1 mg of dried powder was encapsulated in 10 mg
of KBr pellet, in order to prepare translucent sample disc. The powdered sample of
the pellet was loaded in FTIR Spectroscope (Shimadzu, Japan) with a scan range
from 400 to 4000 cm
−1 with a resolution of 4 cm
−1 and the results were recorded
(Arya et al. 2018).
3 Results
3.1 Synthesis of Silver Nanoparticles
The presence of silver nanoparticles in the leaf extract was observed by reduction of
silver ions into silver nanoparticles. The color change of Ethanol extract from green
to reddish brown was observed visually.
3.2 FTIR Analysis of Ethanol Leaf Extracts
Fourier Transform Infra-Red (FTIR) analysis was performed to detect the molecules
present in the ethanol extracts of the leaves. A typical FTIR spectrum of the
compounds is shown in (Fig. 1). The absorption bands were seen at 3367.86, 1634.74,
1393.63, 1057.04, 703.08 and 400.25 cm
−1 . The intense band at 3367.86 corresponds to C = C–H Stretching, the band at 1634.74 corresponds to OH (Absorbed
water), 1393.63 corresponds to t-butyl stretching, band at 1057.04 corresponds to
157
where Ci represents the initial dye concentration and Co represents the final dyeion
concentration.
2.4 Anti-bacterial Activity
The Acetone, Ethanol and Distilled water extracts of Prosopis juliflora, were analyzed
for their anti-bacterial activity for Pseudomonas putida, Bacillus subtilis (Ahmad
et al. 1988; Jeevan et al. 2012).
2.5 Fourier Transform Infra-Red Analysis (FTIR)
Fourier Transform-Infrared Spectrophotometer (FTIR) is a tool to identify types of
chemical compounds in the sample. Dried leaf powder (ethanolic extract) of test
plant was used for FTIR analysis. 1 mg of dried powder was encapsulated in 10 mg
of KBr pellet, in order to prepare translucent sample disc. The powdered sample of
the pellet was loaded in FTIR Spectroscope (Shimadzu, Japan) with a scan range
from 400 to 4000 cm
−1 with a resolution of 4 cm
−1 and the results were recorded
(Arya et al. 2018).
3 Results
3.1 Synthesis of Silver Nanoparticles
The presence of silver nanoparticles in the leaf extract was observed by reduction of
silver ions into silver nanoparticles. The color change of Ethanol extract from green
to reddish brown was observed visually.
3.2 FTIR Analysis of Ethanol Leaf Extracts
Fourier Transform Infra-Red (FTIR) analysis was performed to detect the molecules
present in the ethanol extracts of the leaves. A typical FTIR spectrum of the
compounds is shown in (Fig. 1). The absorption bands were seen at 3367.86, 1634.74,
1393.63, 1057.04, 703.08 and 400.25 cm
−1 . The intense band at 3367.86 corresponds to C = C–H Stretching, the band at 1634.74 corresponds to OH (Absorbed
water), 1393.63 corresponds to t-butyl stretching, band at 1057.04 corresponds to