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S. Sahithya and C. Krishnaveni
sore throat and in healing of wounds. Several alkaloids have been isolated from the
leaf extracts possessing pharmacological properties (Cronk and Fuller 2014). view
of therapeutic importance of this plant, the present research work aims at screening
the silver nanoparticle leaf extracts exploited for dye degradation and antimicrobial
activity. In this way it can easily be eliminated and used in a more meaningful way
(Abiyot and Getachew 2006).
2 Materials and Methods
2.1 Plant Material
The leaves of Prosopis juliflora were collected from Peelamedu, Coimbatore, Tamil
Nadu, India. The leaves of Prosopis juliflora were washed thoroughly, shade dried and
powdered mechanically. The dried leaf material is extracted with different solvents
like acetone, ethanol and distilled water in a Soxhlet apparatus (Abdul et al. 2013).
2.2 Synthesis of Silver Nanoparticles
2 ml of extract was added to the aqueous solution of 2 ml of 2 mM Silver nitrate.
Then the sample was incubated in dark for 24 h. After 24 h, the appearance of
reddish-brown colour indicates the presence of silver nanoparticles. The formation
and completion of silver nanoparticles was characterized by spectrophotometer. The
analysis was carried out in a Visible Spectrometer-Deep vision 2305 with a resolution
of 1 nm between 300 and 600 nm (Raja et al. 2012).
2.3 Dye Degradation Analysis
About 10 mg of methylene blue dye was added to 1000 mL of double distilled
water (stock solution). About 10 mg of the bio-synthesized silver nanoparticles was
added to 100 mL of methylene blue dye solution, this suspension was well mixed
and was put under the sunlight. The absorbance spectrum of the supernatant was
subsequently measured using UV–Vis spectrophotometer at the different wavelength.
Concentration of dye during degradation was calculated by the absorbance value at
660 nm (Vanaja et al. 2014).
Percentage of dye degradation was estimated by the following formula:
%degradation = (Ci − Co/Ci) ∗ 100
(12.1)
S. Sahithya and C. Krishnaveni
sore throat and in healing of wounds. Several alkaloids have been isolated from the
leaf extracts possessing pharmacological properties (Cronk and Fuller 2014). view
of therapeutic importance of this plant, the present research work aims at screening
the silver nanoparticle leaf extracts exploited for dye degradation and antimicrobial
activity. In this way it can easily be eliminated and used in a more meaningful way
(Abiyot and Getachew 2006).
2 Materials and Methods
2.1 Plant Material
The leaves of Prosopis juliflora were collected from Peelamedu, Coimbatore, Tamil
Nadu, India. The leaves of Prosopis juliflora were washed thoroughly, shade dried and
powdered mechanically. The dried leaf material is extracted with different solvents
like acetone, ethanol and distilled water in a Soxhlet apparatus (Abdul et al. 2013).
2.2 Synthesis of Silver Nanoparticles
2 ml of extract was added to the aqueous solution of 2 ml of 2 mM Silver nitrate.
Then the sample was incubated in dark for 24 h. After 24 h, the appearance of
reddish-brown colour indicates the presence of silver nanoparticles. The formation
and completion of silver nanoparticles was characterized by spectrophotometer. The
analysis was carried out in a Visible Spectrometer-Deep vision 2305 with a resolution
of 1 nm between 300 and 600 nm (Raja et al. 2012).
2.3 Dye Degradation Analysis
About 10 mg of methylene blue dye was added to 1000 mL of double distilled
water (stock solution). About 10 mg of the bio-synthesized silver nanoparticles was
added to 100 mL of methylene blue dye solution, this suspension was well mixed
and was put under the sunlight. The absorbance spectrum of the supernatant was
subsequently measured using UV–Vis spectrophotometer at the different wavelength.
Concentration of dye during degradation was calculated by the absorbance value at
660 nm (Vanaja et al. 2014).
Percentage of dye degradation was estimated by the following formula:
%degradation = (Ci − Co/Ci) ∗ 100
(12.1)