156
P. Wankhade et al.
Table 2 Antibacterial test results of samples treated with peppermint oil by LBL technique
Emulsion conc.
and number of
layers
Test culture
No. of colonies
recovered at “0”
hour [B]
No. of colonies
recovered at “24”
hours [A]
Reduction of
microorganisms
[R] in %
Control
S. aureus
2.04 × 10 5
2.12 × 10 7
0
K. pneumoniae 2.08 × 10 5
2.16 × 10 7
0
50 g/l/20 l
S. aureus
1.92 × 10 5
1.2 × 10 4
93.7
K. pneumoniae 1.99 × 10 5
1.7 × 10 4
91.45
100 g/l/20 l
S. aureus
1.52 × 10 5
3 × 10 3
98.02
K. pneumoniae 1.76 × 10 5
4 × 10 3
97.72
100 g/l/20 l and 20
cycles washed
sample
S. aureus
0.65 × 10 5
1.1 × 10 4
83.07
K. pneumoniae 0.70 × 10 5
1.3 × 10 4
81.42
5.3 Antibacterial Test
Antibacterial testing of fabric treated with oil nanoemulsion through LBL technique: Table 2 shows the antibacterial activity of 120 GSM cotton woven scoured
and bleached sample which is treated with 50 and 100 g/l of oil nanoemulsion and
number of layers employed in the LBL technique are 20 (100 g/l/20 l). The sample
treated with 100 g/l/20 l showed highest antibacterial efficiency of 98.02 % against
S. aureus and 97.72 % against K. pneumoniae. The antibacterial efficiency of fabric
sample increased with the increase in oil emulsion concentration. The strong ionic
bonds formed on the cotton surface due to the use of polyelectrolytes in the LBL technique have deposited more oil with the increase in nanoemulsion conc. and number
of layers. The fabric treated with 100 g/l of emulsion has been found to possess its
antibacterial activity till 20 washes, which confirms that the fabric has very good
durability of the finish.
5.4 Mosquito Repellent Testing
Mosquito repellent testing of fabric treated with oil nanoemulsion through LBL
technique: Table 4 shows the mosquito repellency rate of fabrics treated with 50 and
100 concentration of peppermint oil nanoemulsions with 10 and 20 number of layers
used in the LBL technique. The treated fabrics were subjected to excito repellency
test. The fabrics treated with 100 g/l of peppermint oil nanoemulsion and 20 number
of layers gave 100 % mosquito repellency rate. The fabric has been found to possess its
mosquito repellency till 20 washes which confirms that the fabric has good durability
of the finish.
P. Wankhade et al.
Table 2 Antibacterial test results of samples treated with peppermint oil by LBL technique
Emulsion conc.
and number of
layers
Test culture
No. of colonies
recovered at “0”
hour [B]
No. of colonies
recovered at “24”
hours [A]
Reduction of
microorganisms
[R] in %
Control
S. aureus
2.04 × 10 5
2.12 × 10 7
0
K. pneumoniae 2.08 × 10 5
2.16 × 10 7
0
50 g/l/20 l
S. aureus
1.92 × 10 5
1.2 × 10 4
93.7
K. pneumoniae 1.99 × 10 5
1.7 × 10 4
91.45
100 g/l/20 l
S. aureus
1.52 × 10 5
3 × 10 3
98.02
K. pneumoniae 1.76 × 10 5
4 × 10 3
97.72
100 g/l/20 l and 20
cycles washed
sample
S. aureus
0.65 × 10 5
1.1 × 10 4
83.07
K. pneumoniae 0.70 × 10 5
1.3 × 10 4
81.42
5.3 Antibacterial Test
Antibacterial testing of fabric treated with oil nanoemulsion through LBL technique: Table 2 shows the antibacterial activity of 120 GSM cotton woven scoured
and bleached sample which is treated with 50 and 100 g/l of oil nanoemulsion and
number of layers employed in the LBL technique are 20 (100 g/l/20 l). The sample
treated with 100 g/l/20 l showed highest antibacterial efficiency of 98.02 % against
S. aureus and 97.72 % against K. pneumoniae. The antibacterial efficiency of fabric
sample increased with the increase in oil emulsion concentration. The strong ionic
bonds formed on the cotton surface due to the use of polyelectrolytes in the LBL technique have deposited more oil with the increase in nanoemulsion conc. and number
of layers. The fabric treated with 100 g/l of emulsion has been found to possess its
antibacterial activity till 20 washes, which confirms that the fabric has very good
durability of the finish.
5.4 Mosquito Repellent Testing
Mosquito repellent testing of fabric treated with oil nanoemulsion through LBL
technique: Table 4 shows the mosquito repellency rate of fabrics treated with 50 and
100 concentration of peppermint oil nanoemulsions with 10 and 20 number of layers
used in the LBL technique. The treated fabrics were subjected to excito repellency
test. The fabrics treated with 100 g/l of peppermint oil nanoemulsion and 20 number
of layers gave 100 % mosquito repellency rate. The fabric has been found to possess its
mosquito repellency till 20 washes which confirms that the fabric has good durability
of the finish.