The large majority of NOD domains of fungal NLRs are either of the NACHT or NB-ARC
type, with NACHT being more frequent than
NB-ARC (in a ~ 3:1 ratio) (Fig. 6.2a, middle
chart) (Dyrka et al. 2014). Superstructureforming repeats (SSFRs) are not LRRs as
found in most plant and animal NLRs but of
the ANK, TPR, and WD type in about equal
prevalence (Dyrka et al. 2014) (Fig. 6.2a, right
chart). ANK-repeats are more abundant in
Ascomycota and WD-repeats in Basidiomycota. There is a preferential association of NBARC with TPR-repeats and of NACHT with
ANK or WD-repeats. A variety of effector
Fig. 6.2 Domain annotation of NLRs from fungal genomes. (a) Each of the pie charts shows the proportions
of the indicated domain annotations in a total of 36,141
NLR proteins identified in fungal genomes. Proportions are given as a fraction of the domain that has
received a given annotation over the total number of
annotated domains in the N-terminal (left chart), NBD
(middle chart), and C-terminal domain (right chart).
(b) For each phylogenetic group, the total number of
analyzed genomes (#projects) and the number of genomes with at least one NLR (#prj w/nlr) are given, as
well as a semi-quantitative representation of the NLR
abundance and specific N-terminal domain annotation
in each phylogenetic group. GBL, Goodbye-like (overlaps with Pfam Goodbye (PF17109) and NACHT_N
(PF17100)); HLL, HeLo-like (overlaps with Pfam
STAND_N clan (CL0587) including HeLo_like_N
(PF17111), SesA (PF17107)); SBL, sesb-like (overlaps
with Pfam AB_hydrolase clan (CL0028)) (Dyrka et al.
2014); PNP, PNP_UDP_1 (PF01048); HET (PF06985);
PAT, Pfam Patatin clan (CL0323); HEL, HeLo
(PF14479); PFD, prion-forming domain like (overlaps
with Pfam HET-S (PF17108), NACHT_sigma
(PF17106), and ses_B (PF17046)) (Dyrka et al. 2014);
C2 (PF00168); REL, RelA_SpoT (PF04607); CHAT
(PF12770); PS8, Peptidase_S8 (PF00082); PKN, Pfam
Pkinase clan (CL0016); and TIR, TIR_2 (PF01582,
PF13676). The open circle denotes a fraction of genomes with NLR/domain annotation ¼ 0, the first quadrant a fraction >0 and < 0.25, half-filled symbol a
fraction 0.25 and < 0.75, third quadrant a fraction
0.75 and < 1, and the filled symbol a fraction ¼ 1
128
A. Daskalov et al.
type, with NACHT being more frequent than
NB-ARC (in a ~ 3:1 ratio) (Fig. 6.2a, middle
chart) (Dyrka et al. 2014). Superstructureforming repeats (SSFRs) are not LRRs as
found in most plant and animal NLRs but of
the ANK, TPR, and WD type in about equal
prevalence (Dyrka et al. 2014) (Fig. 6.2a, right
chart). ANK-repeats are more abundant in
Ascomycota and WD-repeats in Basidiomycota. There is a preferential association of NBARC with TPR-repeats and of NACHT with
ANK or WD-repeats. A variety of effector
Fig. 6.2 Domain annotation of NLRs from fungal genomes. (a) Each of the pie charts shows the proportions
of the indicated domain annotations in a total of 36,141
NLR proteins identified in fungal genomes. Proportions are given as a fraction of the domain that has
received a given annotation over the total number of
annotated domains in the N-terminal (left chart), NBD
(middle chart), and C-terminal domain (right chart).
(b) For each phylogenetic group, the total number of
analyzed genomes (#projects) and the number of genomes with at least one NLR (#prj w/nlr) are given, as
well as a semi-quantitative representation of the NLR
abundance and specific N-terminal domain annotation
in each phylogenetic group. GBL, Goodbye-like (overlaps with Pfam Goodbye (PF17109) and NACHT_N
(PF17100)); HLL, HeLo-like (overlaps with Pfam
STAND_N clan (CL0587) including HeLo_like_N
(PF17111), SesA (PF17107)); SBL, sesb-like (overlaps
with Pfam AB_hydrolase clan (CL0028)) (Dyrka et al.
2014); PNP, PNP_UDP_1 (PF01048); HET (PF06985);
PAT, Pfam Patatin clan (CL0323); HEL, HeLo
(PF14479); PFD, prion-forming domain like (overlaps
with Pfam HET-S (PF17108), NACHT_sigma
(PF17106), and ses_B (PF17046)) (Dyrka et al. 2014);
C2 (PF00168); REL, RelA_SpoT (PF04607); CHAT
(PF12770); PS8, Peptidase_S8 (PF00082); PKN, Pfam
Pkinase clan (CL0016); and TIR, TIR_2 (PF01582,
PF13676). The open circle denotes a fraction of genomes with NLR/domain annotation ¼ 0, the first quadrant a fraction >0 and < 0.25, half-filled symbol a
fraction 0.25 and < 0.75, third quadrant a fraction
0.75 and < 1, and the filled symbol a fraction ¼ 1
128
A. Daskalov et al.