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4. Han X, Liu Z, Jo MC, Zhang K, Li Y, Zeng Z,
Li N, Zu Y, Qin L (2015) CRISPR-Cas9 delivery to hard-to-transfect cells via membrane
deformation.
Sci
Adv 1(7):e1500454.
https://doi.org/10.1126/sciadv.1500454
5. Kim TK, Eberwine JH (2010) Mammalian cell
transfection: the present and the future. Anal
Bioanal Chem 397(8):3173–3178. https://
doi.org/10.1007/s00216-010-3821-6
6. Potter H, Heller R (2010) Transfection by
electroporation. Curr Protoc Mol Biol
Chapter 9:Unit9 3. doi:https://doi.org/10.
1002/0471142727.mb0903s92
7. Buschle M, Brenner MK, Chen IS, Drexler
HG, Gignac SM, Rooney CM (1990) Transfection and gene expression in normal and
malignant primary B lymphocytes. J Immunol
Methods 133(1):77–85
8. Seiffert M, Stilgenbauer S, Dohner H, Lichter
P (2007) Efficient nucleofection of primary
human B cells and B-CLL cells induces apoptosis, which depends on the microenvironment
and on the structure of transfected nucleic
acids. Leukemia 21(9):1977–1983. https://
doi.org/10.1038/sj.leu.2404863
9. Yu J, Chen Y, Chen L, Zhang L, Rassenti LZ,
Widhopf GF 2nd, Kipps TJ (2018) Cirmtuzumab inhibits ibrutinib-resistant, Wnt5ainduced Rac1 activation and proliferation in
mantle cell lymphoma. Oncotarget 9
(37):24731–24736.
https://doi.org/10.
18632/oncotarget.25340
10. Fukuda T, Chen L, Endo T, Tang L, Lu D,
Castro JE, Widhopf GF 2nd, Rassenti LZ,
Cantwell MJ, Prussak CE, Carson DA, Kipps
TJ (2008) Antisera induced by infusions of
autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor
for Wnt5a. Proc Natl Acad Sci U S A 105
(8):3047–3052. https://doi.org/10.1073/
pnas.0712148105
11. Choudhury A, Derkow K, Daneshmanesh AH,
Mikaelsson E, Kiaii S, Kokhaei P, Osterborg A,
Mellstedt H (2010) Silencing of ROR1 and
FMOD with siRNA results in apoptosis of
CLL cells. Br J Haematol 151(4):327–335.
https://doi.org/10.1111/j.1365-2141.2010.
08362.x
90
Jian Yu
(11):2281–2308. https://doi.org/10.1038/
nprot.2013.143
4. Han X, Liu Z, Jo MC, Zhang K, Li Y, Zeng Z,
Li N, Zu Y, Qin L (2015) CRISPR-Cas9 delivery to hard-to-transfect cells via membrane
deformation.
Sci
Adv 1(7):e1500454.
https://doi.org/10.1126/sciadv.1500454
5. Kim TK, Eberwine JH (2010) Mammalian cell
transfection: the present and the future. Anal
Bioanal Chem 397(8):3173–3178. https://
doi.org/10.1007/s00216-010-3821-6
6. Potter H, Heller R (2010) Transfection by
electroporation. Curr Protoc Mol Biol
Chapter 9:Unit9 3. doi:https://doi.org/10.
1002/0471142727.mb0903s92
7. Buschle M, Brenner MK, Chen IS, Drexler
HG, Gignac SM, Rooney CM (1990) Transfection and gene expression in normal and
malignant primary B lymphocytes. J Immunol
Methods 133(1):77–85
8. Seiffert M, Stilgenbauer S, Dohner H, Lichter
P (2007) Efficient nucleofection of primary
human B cells and B-CLL cells induces apoptosis, which depends on the microenvironment
and on the structure of transfected nucleic
acids. Leukemia 21(9):1977–1983. https://
doi.org/10.1038/sj.leu.2404863
9. Yu J, Chen Y, Chen L, Zhang L, Rassenti LZ,
Widhopf GF 2nd, Kipps TJ (2018) Cirmtuzumab inhibits ibrutinib-resistant, Wnt5ainduced Rac1 activation and proliferation in
mantle cell lymphoma. Oncotarget 9
(37):24731–24736.
https://doi.org/10.
18632/oncotarget.25340
10. Fukuda T, Chen L, Endo T, Tang L, Lu D,
Castro JE, Widhopf GF 2nd, Rassenti LZ,
Cantwell MJ, Prussak CE, Carson DA, Kipps
TJ (2008) Antisera induced by infusions of
autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor
for Wnt5a. Proc Natl Acad Sci U S A 105
(8):3047–3052. https://doi.org/10.1073/
pnas.0712148105
11. Choudhury A, Derkow K, Daneshmanesh AH,
Mikaelsson E, Kiaii S, Kokhaei P, Osterborg A,
Mellstedt H (2010) Silencing of ROR1 and
FMOD with siRNA results in apoptosis of
CLL cells. Br J Haematol 151(4):327–335.
https://doi.org/10.1111/j.1365-2141.2010.
08362.x
90
Jian Yu