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https://doi.org/10.
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100
Untransfected JEKO-1
Transfected JEKO-1
b
a
FL1-H: GFP
GFP + : 0.0%
GFP + : 17.8%
FL1-H: GFP
% of Max
% of Max
80
60
40
20
0
100
80
60
40
20
0
10 0
10 1
10 2
10 3
10 4
10 0
10 1
10 2
10 3
10 4
Fig. 1 Flow cytometry analysis of GFP
+ JEKO-1 cells after electroporation. (a) GFP expression in JEKO-1 cells.
Un-transfected JEKO-1 cells were used as negative control, in which GFP were undetectable. (b) Successfully
transfected JEKO-1 cells. The percentage of GFP
+ JEKO-1 cells was 17.8% in the transfected cells after gating
out PI
+ dead cells
100
80
60
Isotype
Anti-ROR1
Isotype
Anti-ROR1
20
40
0
100
80
60
20
40
0
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
% of Max
% of Max
ROR1 – JEKO-1
ROR1 + JEKO-1
a
b
FL4–H: ROR1
FL4–H: ROR1
Fig. 2 Flow cytometry analysis of ROR1 expression in JEKO-1 cells after single colon screening. (a) ROR1
expression in wild-type ROR1
+ JEKO-1 cells staining with Alexa 647-conjugated anti-ROR1 antibody. (b) ROR1
expression in ROR1
À JEKO-1 cells staining with Alexa 647-conjugated anti-ROR1 antibody
Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-Function. . .
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