12 Targeted In Vivo Electroporation Using Nanoengineered
Microelectrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Daniel Schwarz and Andreas T. Schaefer
13 Zygote Electroporation for CRISPR/Cas9 Delivery to Generate
Genetically Modified Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Tatsuya Takemoto
14 Genome Editing in Staphylococcus aureus by Conditional
Recombineering and CRISPR/Cas9-Mediated Counterselection . . . . . . . . . . . . . 127
Kelsi Penewit and Stephen J. Salipante
15 Isolation and Nanoscale Electroporation of Primary Neuronal
Cultures In Situ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Diego Alzate-Correa, William Lawrence, Natalia Higuita-Castro,
and Daniel Gallego-Perez
PART IV MICROORGANISM ELECTROPORATION
16 Transformation of the Model Microalga Chlamydomonas reinhardtii
Without Cell-Wall Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Takashi Yamano and Hideya Fukuzawa
17 Electroporation Transformation Protocol for Phaeodactylum tricornutum . . . . . 163
Hanhua Hu and Yufang Pan
18 Efficient Transformation of the Diatoms Phaeodactylum tricornutum
by Multipulse Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Kentaro Ifuku and Dongyi Yan
19 Rapid and Effective Electroporation Protocol
for Nannochloropsis oceanica. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Da-Wei Li, Srinivasan Balamurugan, Jian-Wei Zheng,
Wei-Dong Yang, Jie-Sheng Liu, and Hong-Ye Li
20 Electroporation Procedures for Genetic Modification
of Green Algae (Chlorella spp.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Liang Ji and Jianhua Fan
21 Electrotransformation of Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
E. Meilhoc and J. Teissie
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
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Microelectrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Daniel Schwarz and Andreas T. Schaefer
13 Zygote Electroporation for CRISPR/Cas9 Delivery to Generate
Genetically Modified Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Tatsuya Takemoto
14 Genome Editing in Staphylococcus aureus by Conditional
Recombineering and CRISPR/Cas9-Mediated Counterselection . . . . . . . . . . . . . 127
Kelsi Penewit and Stephen J. Salipante
15 Isolation and Nanoscale Electroporation of Primary Neuronal
Cultures In Situ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Diego Alzate-Correa, William Lawrence, Natalia Higuita-Castro,
and Daniel Gallego-Perez
PART IV MICROORGANISM ELECTROPORATION
16 Transformation of the Model Microalga Chlamydomonas reinhardtii
Without Cell-Wall Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Takashi Yamano and Hideya Fukuzawa
17 Electroporation Transformation Protocol for Phaeodactylum tricornutum . . . . . 163
Hanhua Hu and Yufang Pan
18 Efficient Transformation of the Diatoms Phaeodactylum tricornutum
by Multipulse Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Kentaro Ifuku and Dongyi Yan
19 Rapid and Effective Electroporation Protocol
for Nannochloropsis oceanica. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Da-Wei Li, Srinivasan Balamurugan, Jian-Wei Zheng,
Wei-Dong Yang, Jie-Sheng Liu, and Hong-Ye Li
20 Electroporation Procedures for Genetic Modification
of Green Algae (Chlorella spp.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Liang Ji and Jianhua Fan
21 Electrotransformation of Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
E. Meilhoc and J. Teissie
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
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