Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
PART I DESIGN AND FABRICATION OF MICRO-/NANO-ELECTROPORATION
1 Cell Size-Specific Transfection by Micropillar Array Electroporation. . . . . . . . . . .
3
Xuan Liu, Yingbo Zu, and Shengnian Wang
2 Soft Electroporation Through 3D Hollow Nanoelectrodes. . . . . . . . . . . . . . . . . . . 13
Jun Yin and Yang Li
3 Micromachining of Polymeric Microfluidic
Micro/Nanoelectroporation Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Lei Li
4 3D Nanochannel Array for High-Throughput Cell Manipulation
and Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Lingqian Chang, Stephen Black, Chandani Chitrakar,
and Mehdi Nouri
5 Low-Voltage Flow-Through Electroporation Membrane and Method . . . . . . . . . 43
Juliette Experton, Aaron G. Wilson, and Charles R. Martin
PART II MICRO-/NANO-ELECTROPORATION FOR IN VITRO DELIVERY
6 Nanofountain Probe Electroporation for Monoclonal
Cell Line Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Horacio D. Espinosa, Prithvijit Mukherjee, and Cesar Patino
7 3D Nanochannel Electroporation for Macromolecular
Nucleotide Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Lingqian Chang, Chandani Chitrakar, and Mehdi Nouri
8 Nanoelectroporation and Collection of Genetically Modified
Exosomes in Primary Cultures of Dendritic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Silvia Duarte-Sanmiguel, Natalia Higuita-Castro,
and Daniel Gallego-Perez
9 Electroporation of CRISPR-Cas9 into Malignant B Cells
for Loss-of-Function Studies of Target Gene Via Knockout . . . . . . . . . . . . . . . . . . 85
Jian Yu
10 Microfluidic Device for Localized Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Justin Brooks, Arian Jaberi, and Ruiguo Yang
PART III ADVANCED IN VIVO ELECTROPORATION
11 Transdermal Delivery of Nucleic Acid Mediated by Punching
and Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Dong Huang, Yuanyu Huang, and Zhihong Li
vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
PART I DESIGN AND FABRICATION OF MICRO-/NANO-ELECTROPORATION
1 Cell Size-Specific Transfection by Micropillar Array Electroporation. . . . . . . . . . .
3
Xuan Liu, Yingbo Zu, and Shengnian Wang
2 Soft Electroporation Through 3D Hollow Nanoelectrodes. . . . . . . . . . . . . . . . . . . 13
Jun Yin and Yang Li
3 Micromachining of Polymeric Microfluidic
Micro/Nanoelectroporation Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Lei Li
4 3D Nanochannel Array for High-Throughput Cell Manipulation
and Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Lingqian Chang, Stephen Black, Chandani Chitrakar,
and Mehdi Nouri
5 Low-Voltage Flow-Through Electroporation Membrane and Method . . . . . . . . . 43
Juliette Experton, Aaron G. Wilson, and Charles R. Martin
PART II MICRO-/NANO-ELECTROPORATION FOR IN VITRO DELIVERY
6 Nanofountain Probe Electroporation for Monoclonal
Cell Line Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Horacio D. Espinosa, Prithvijit Mukherjee, and Cesar Patino
7 3D Nanochannel Electroporation for Macromolecular
Nucleotide Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Lingqian Chang, Chandani Chitrakar, and Mehdi Nouri
8 Nanoelectroporation and Collection of Genetically Modified
Exosomes in Primary Cultures of Dendritic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Silvia Duarte-Sanmiguel, Natalia Higuita-Castro,
and Daniel Gallego-Perez
9 Electroporation of CRISPR-Cas9 into Malignant B Cells
for Loss-of-Function Studies of Target Gene Via Knockout . . . . . . . . . . . . . . . . . . 85
Jian Yu
10 Microfluidic Device for Localized Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Justin Brooks, Arian Jaberi, and Ruiguo Yang
PART III ADVANCED IN VIVO ELECTROPORATION
11 Transdermal Delivery of Nucleic Acid Mediated by Punching
and Electroporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Dong Huang, Yuanyu Huang, and Zhihong Li
vii