INDEX
A
Adoptive immunotherapy........................... 70, 71, 73, 74
Algal cell transformation............................................... 175
C
Cardiomyocytes transfection ............................. 71, 74, 75
Cell-based immunotherapies .......................................... 79
Cell line generation...................................................59–67
Cell reprogramming.................................... 69, 71, 73, 74
Centrifugal force cell trapping .................................23, 25
Chaetoceros gracilis ............................................... 170, 173
Chlamydomonas reinhardtii................155–159, 175, 184
Chlorella................................................................ 181–185
CRISPR/Cas9................. 59, 85, 86, 121–125, 127–141
D
Dendritic cells............................................................79–83
Diatoms ........................................................163, 169–173
Dielectrophoresis (DEP) ............................ 31, 32, 40, 75
DNA combing and imprinting (DCI) .............. 23, 25, 26
DNA delivery ..................................................... 3–11, 175
E
Electric field gradients ....................................... 32, 43, 44
Electroporation ...................................................... v, 3–11,
13–18, 21–27, 29–40, 43–45, 47–51, 59–67,
69–76, 85–89, 91–96, 101–111, 113–119,
121–125, 128, 130, 135, 136, 139, 141,
145–148, 155, 157–159, 163–166, 169–173,
175–179, 181–185
Electrotransformation................ 181, 182, 184, 187–192
Escherichia coli ................................................... 43–46, 48,
49, 53, 128, 129, 132, 134–138, 146, 165, 190
Exogenous DNA........................................................... 159
F
Flexible electrode .......................................................... 109
Focused ion beam (FIB) milling ........................... 15, 116
G
Gene delivery................................................ v, 23, 80, 146
Gene electrotransfer (GET)..............................v, 187–191
Genetic engineering .........................................70, 80, 176
Genetic transformation ........................................ 163, 169
Genome editing .................................. 121–125, 127–141
Gold-microtube membrane............................... 43–46, 49
I
In vivo ........................................... 13, 102, 103, 113–119
Isogenic strains ............................................ 127, 128, 139
J
JEKO-1......................................................................87–89
M
Macromolecular nucleotides ....................................69–76
Magnetic tweezers (MT) ...................... 22, 31–34, 36–37
Micro-contact printing ...................................... 60, 64, 67
Microelectrodes............................................................102,
106–108, 113–119
Microfabrication................................................... 102–104
Microfluidics............................................................ 14, 16,
17, 21–27, 31–33, 35, 38, 91–96
Micromachining ........................................................21–27
Microneedle......................................................... 102–104,
106, 108–111
Micropillar array .......................................................... 3–11
Monoclonal cell line..................................................59–67
Multipulse.....................................................164, 169–173
N
Nannochloropsis ............................................163, 175–179
Nanochannel array ....................................................29–40
Nanochannel-based electroporation (NEP) ................. 80,
146–148, 150
Nanochannel electroporation (NEP)............................ 21,
23, 30–36, 38–40, 69–76, 80, 82, 83
Nano-electroporation ..................................21–27, 30, 75
Nanoengineering.................................................. 113–119
Nanofabrication.........................................................14–16
Nanofountain probe electroporation
(NFP-E) .........................................................59–67
Non-viral gene delivery .................................................... 3
Nuclei acid delivery.............................................. 101–111
Nucleofector..............................................................86–88
Shulin Li et al. (eds.), Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice,
Methods in Molecular Biology, vol. 2050, https://doi.org/10.1007/978-1-4939-9740-4,
© Springer Science+Business Media, LLC, part of Springer Nature 2020
195
A
Adoptive immunotherapy........................... 70, 71, 73, 74
Algal cell transformation............................................... 175
C
Cardiomyocytes transfection ............................. 71, 74, 75
Cell-based immunotherapies .......................................... 79
Cell line generation...................................................59–67
Cell reprogramming.................................... 69, 71, 73, 74
Centrifugal force cell trapping .................................23, 25
Chaetoceros gracilis ............................................... 170, 173
Chlamydomonas reinhardtii................155–159, 175, 184
Chlorella................................................................ 181–185
CRISPR/Cas9................. 59, 85, 86, 121–125, 127–141
D
Dendritic cells............................................................79–83
Diatoms ........................................................163, 169–173
Dielectrophoresis (DEP) ............................ 31, 32, 40, 75
DNA combing and imprinting (DCI) .............. 23, 25, 26
DNA delivery ..................................................... 3–11, 175
E
Electric field gradients ....................................... 32, 43, 44
Electroporation ...................................................... v, 3–11,
13–18, 21–27, 29–40, 43–45, 47–51, 59–67,
69–76, 85–89, 91–96, 101–111, 113–119,
121–125, 128, 130, 135, 136, 139, 141,
145–148, 155, 157–159, 163–166, 169–173,
175–179, 181–185
Electrotransformation................ 181, 182, 184, 187–192
Escherichia coli ................................................... 43–46, 48,
49, 53, 128, 129, 132, 134–138, 146, 165, 190
Exogenous DNA........................................................... 159
F
Flexible electrode .......................................................... 109
Focused ion beam (FIB) milling ........................... 15, 116
G
Gene delivery................................................ v, 23, 80, 146
Gene electrotransfer (GET)..............................v, 187–191
Genetic engineering .........................................70, 80, 176
Genetic transformation ........................................ 163, 169
Genome editing .................................. 121–125, 127–141
Gold-microtube membrane............................... 43–46, 49
I
In vivo ........................................... 13, 102, 103, 113–119
Isogenic strains ............................................ 127, 128, 139
J
JEKO-1......................................................................87–89
M
Macromolecular nucleotides ....................................69–76
Magnetic tweezers (MT) ...................... 22, 31–34, 36–37
Micro-contact printing ...................................... 60, 64, 67
Microelectrodes............................................................102,
106–108, 113–119
Microfabrication................................................... 102–104
Microfluidics............................................................ 14, 16,
17, 21–27, 31–33, 35, 38, 91–96
Micromachining ........................................................21–27
Microneedle......................................................... 102–104,
106, 108–111
Micropillar array .......................................................... 3–11
Monoclonal cell line..................................................59–67
Multipulse.....................................................164, 169–173
N
Nannochloropsis ............................................163, 175–179
Nanochannel array ....................................................29–40
Nanochannel-based electroporation (NEP) ................. 80,
146–148, 150
Nanochannel electroporation (NEP)............................ 21,
23, 30–36, 38–40, 69–76, 80, 82, 83
Nano-electroporation ..................................21–27, 30, 75
Nanoengineering.................................................. 113–119
Nanofabrication.........................................................14–16
Nanofountain probe electroporation
(NFP-E) .........................................................59–67
Non-viral gene delivery .................................................... 3
Nuclei acid delivery.............................................. 101–111
Nucleofector..............................................................86–88
Shulin Li et al. (eds.), Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice,
Methods in Molecular Biology, vol. 2050, https://doi.org/10.1007/978-1-4939-9740-4,
© Springer Science+Business Media, LLC, part of Springer Nature 2020
195