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© The Editor(s) (if applicable) and The Author(s), under exclusive license to
Springer Nature Switzerland AG 2021
S. Fourmentin et al. (eds.), Deep Eutectic Solvents for Medicine, Gas
Solubilization and Extraction of Natural Substances, Environmental Chemistry
for a Sustainable World 56, https://doi.org/10.1007/978-3-030-53069-3_6
Chapter 6
Methods for Extraction of Bioactive
Compounds from Plant and Animal
Matter Using Deep Eutectic Solvents
Lamia Nakhle, Miriana Kfoury, Isabelle Mallard, David Landy,
and Hélène Greige-Gerges
Contents
6.1 Introduction
184
6.2 Microextraction Techniques: Description and Application with Deep Eutectic
Solvents
186
6.2.1 Liquid-Phase Microextraction
187
6.2.2 Ultrasound Microextraction
197
6.2.3 Microwave Extraction
198
6.2.4 Vortex Extraction
199
6.2.5 Heating and Stirring
204
6.2.6 Solid-Phase Microextraction
205
6.2.7 Combined Extraction Techniques
216
6.3 Influence of Deep Eutectic Solvent Properties on the Extraction Efficiency
224
6.3.1 Melting Point
224
6.3.2 Density
224
6.3.3 Viscosity
225
6.3.4 Compatibility with Instrumental Detection Systems
227
6.3.5 Surface Tension
227
L. Nakhle
Bioactive Molecules Research Laboratory, Faculty of Sciences, Lebanese University,
Jdaidet el-Matn, Lebanon
Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV, UR 4492), SFR
Condorcet FR CNRS 3417, Université du Littoral Côte d’Opale (ULCO), Dunkerque, France
e-mail: lamia.nakhle@univ-littoral.fr
M. Kfoury · H. Greige-Gerges (*)
Bioactive Molecules Research Laboratory, Faculty of Sciences, Lebanese University,
Jdaidet el-Matn, Lebanon
e-mail: hgreige@ul.edu.lb
I. Mallard · D. Landy
Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV, UR 4492), SFR
Condorcet FR CNRS 3417, Université du Littoral Côte d’Opale (ULCO), Dunkerque, France
e-mail: isabelle.mallard@univ-littoral.fr; david.landy@univ-littoral.fr