114
Table 3.2 Summary of potential methods of characterization of therapeutic deep eutectic systems (THEDES)
Characterization method
Identification
and morphology
Molecular
structure
Hydrogen
bonding
interactions
Thermal
stability
Phase
transitions
Melting point,
glass
transition
Bioavailability
In vitro
cytotoxicity
Polarized optical microscopy
√
1
H nuclear magnetic resonance
(2D techniques)
√
√
√
Differential scanning
calorimetry
√
√
√
√
Thermogravimetric analysis
√
√
√
Fourier-transform infrared
spectroscopy/attenuated total
reflection
√
√
√
Water content
√
Solubility
√
Permeability
√
Viscosity
√
Density
√
Polarity
√
MTS/MTT/Alamar Blue (cell
viability assays)
√
F. Santos and A. R. C. Duarte