94. Sharma M, Mehta S, Kumar A (2013) Purification and characterization of alkaline xylanase
secreted from Paenibacillus macquariensis. Adv Microbiol 3:32–41
95. Beg QK, Bhushan B, Kapoor M, Hoondal GS (2000) Production and characterization of
thermostable xylanase and pectinase from Streptomyces sp. QG-11-3. J Ind Microbiol
Biotechnol 24:396–402
96. Raj A, Kumar S, Singh SK (2013) A highly thermostable xylanase from Stenotrophomonas
maltophilia: purification and partial characterization. Enzyme Res 2013:429305. https://doi.
org/10.1155/2013/429305
97. Zhang F, Chen JJ, Ren WZ, Lin LB, Zhou Y, Zhi XY et al (2012) Cloning, expression, and
characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans
YIM 90462T. J Ind Microbiol Biotechnol 39:1109–1116
98. Honda H, Kudo T, Ikura Y, Horikoshi K (1985) Two types of xylanases of alkalophilic
Bacillus sp. No. C-125. Can J Microbiol 31:538–542
99. Herlet J, Kornberger P, Roessler B, Glanz J, Schwarz WH, Liebl W, Zverlov VV (2017) A
new method to evaluate temperature vs. pH activity profiles for biotechnological relevant
enzymes. Biotechnol Biofuels 10:234
100. Takeda N, Hirasawa K, Uchimura K, Nogi Y, Hatada Y, Usami R et al (2004) Purification and
enzymatic properties of a highly alkaline mannanase from alkaliphilic Bacillus sp. strain
JAMB-750. J Biol Macromol 4:67–74
101. Takeda N, Hirasawa K, Uchimura K, Nogi Y, Hatada Y, Akita M et al (2004) Alkaline
mannanase from a novel species of alkaliphilic Bacillus. J Appl Glycosci 51:229–236
102. Vijayalaxmi S, Prakash P, Jayalakshmi SK, Mulimani VH, Sreeramulu K (2013) Production of
extremely alkaliphilic, halotolerant, detergent, and thermostable mannanase by the free and
immobilized cells of Bacillus halodurans PPKS-2. Purification and characterization. Appl
Biochem Biotechnol 171:382–395
103. Zhou C, Xue Y, Ma Y (2018) Characterization and high-efficiency secreted expression in
Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain
S10. Microb Cell Factories 17:124
104. Ma Y, Xue Y, Dou Y, Xu Z, Tao W, Zhou P (2004) Characterization and gene cloning of a
novel β-mannanase from alkaliphilic Bacillus sp. N16-5. Extremophiles 8:447–454
105. Chauhan PS, Sharma P, Puri N, Gupta N (2014) Purification and characterization of an alkalithermostable β-mannanase from Bacillus nealsonii PN-11 and its application in
mannooligosaccharides preparation having prebiotic potential. Eur Food Res Technol
238:927–936
106. Pradeep GC, Cho SS, Choi YH, Choi YS, Jee JP, Seong CN et al (2016) An extremely alkaline
mannanase from Streptomyces sp. CS428 hydrolyzes galactomannan producing series of
mannooligosaccharides. World J Microbiol Biotechnol 32:84. https://doi.org/10.1007/
s11274-016-2040-5
107. Akino T, Nakamura N, Horikoshi K (1988) Characterization of three β-mannanases of an
alkalophilic Bacillus sp. Agric Biol Chem 52:773–779
108. El-Sharouny EE, El-Toukhy NM, El-Sersy NA, El-Gayar AA (2015) Optimization and
purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1
isolated from Bani Salama Lake in Wadi El-Natron. Biotechnol Biotechnol Equip 29:315–323
109. Yang P, Li Y, Wang Y, Meng K, Luo H, Yuan T et al (2009) A novel beta-mannanase with
high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and
enzymatic characterization. Appl Biochem Biotechnol 159:85–94
110. Li Y, Yang P, Meng K, Wang Y, Luo H, Wu N et al (2008) Gene cloning, expression, and
characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416.
J Microbiol Biotechnol 18:160–166
111. Kumar D, Angural S, Rana M, Kaur G, Puri N, Gupta N (2017) Cloning, characterization and
its potential in pulp bio bleaching by alkali thermostable β-mannanase from Bacillus sp. 22.
Eur J Pharm Med Res 4:584–592
Alkaline Active Hemicellulases
285
secreted from Paenibacillus macquariensis. Adv Microbiol 3:32–41
95. Beg QK, Bhushan B, Kapoor M, Hoondal GS (2000) Production and characterization of
thermostable xylanase and pectinase from Streptomyces sp. QG-11-3. J Ind Microbiol
Biotechnol 24:396–402
96. Raj A, Kumar S, Singh SK (2013) A highly thermostable xylanase from Stenotrophomonas
maltophilia: purification and partial characterization. Enzyme Res 2013:429305. https://doi.
org/10.1155/2013/429305
97. Zhang F, Chen JJ, Ren WZ, Lin LB, Zhou Y, Zhi XY et al (2012) Cloning, expression, and
characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans
YIM 90462T. J Ind Microbiol Biotechnol 39:1109–1116
98. Honda H, Kudo T, Ikura Y, Horikoshi K (1985) Two types of xylanases of alkalophilic
Bacillus sp. No. C-125. Can J Microbiol 31:538–542
99. Herlet J, Kornberger P, Roessler B, Glanz J, Schwarz WH, Liebl W, Zverlov VV (2017) A
new method to evaluate temperature vs. pH activity profiles for biotechnological relevant
enzymes. Biotechnol Biofuels 10:234
100. Takeda N, Hirasawa K, Uchimura K, Nogi Y, Hatada Y, Usami R et al (2004) Purification and
enzymatic properties of a highly alkaline mannanase from alkaliphilic Bacillus sp. strain
JAMB-750. J Biol Macromol 4:67–74
101. Takeda N, Hirasawa K, Uchimura K, Nogi Y, Hatada Y, Akita M et al (2004) Alkaline
mannanase from a novel species of alkaliphilic Bacillus. J Appl Glycosci 51:229–236
102. Vijayalaxmi S, Prakash P, Jayalakshmi SK, Mulimani VH, Sreeramulu K (2013) Production of
extremely alkaliphilic, halotolerant, detergent, and thermostable mannanase by the free and
immobilized cells of Bacillus halodurans PPKS-2. Purification and characterization. Appl
Biochem Biotechnol 171:382–395
103. Zhou C, Xue Y, Ma Y (2018) Characterization and high-efficiency secreted expression in
Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain
S10. Microb Cell Factories 17:124
104. Ma Y, Xue Y, Dou Y, Xu Z, Tao W, Zhou P (2004) Characterization and gene cloning of a
novel β-mannanase from alkaliphilic Bacillus sp. N16-5. Extremophiles 8:447–454
105. Chauhan PS, Sharma P, Puri N, Gupta N (2014) Purification and characterization of an alkalithermostable β-mannanase from Bacillus nealsonii PN-11 and its application in
mannooligosaccharides preparation having prebiotic potential. Eur Food Res Technol
238:927–936
106. Pradeep GC, Cho SS, Choi YH, Choi YS, Jee JP, Seong CN et al (2016) An extremely alkaline
mannanase from Streptomyces sp. CS428 hydrolyzes galactomannan producing series of
mannooligosaccharides. World J Microbiol Biotechnol 32:84. https://doi.org/10.1007/
s11274-016-2040-5
107. Akino T, Nakamura N, Horikoshi K (1988) Characterization of three β-mannanases of an
alkalophilic Bacillus sp. Agric Biol Chem 52:773–779
108. El-Sharouny EE, El-Toukhy NM, El-Sersy NA, El-Gayar AA (2015) Optimization and
purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1
isolated from Bani Salama Lake in Wadi El-Natron. Biotechnol Biotechnol Equip 29:315–323
109. Yang P, Li Y, Wang Y, Meng K, Luo H, Yuan T et al (2009) A novel beta-mannanase with
high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and
enzymatic characterization. Appl Biochem Biotechnol 159:85–94
110. Li Y, Yang P, Meng K, Wang Y, Luo H, Wu N et al (2008) Gene cloning, expression, and
characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416.
J Microbiol Biotechnol 18:160–166
111. Kumar D, Angural S, Rana M, Kaur G, Puri N, Gupta N (2017) Cloning, characterization and
its potential in pulp bio bleaching by alkali thermostable β-mannanase from Bacillus sp. 22.
Eur J Pharm Med Res 4:584–592
Alkaline Active Hemicellulases
285