tOmpA, see OmpA (E. coli) transmembrane domain
Torpedo marmorata, 288
post-synaptic membrane fragments, 39
as source of nAChR, 39
Toyoshima, C., 47
Transducin
interaction with ND-trapped monomeric
rhodopsin, 116
Transfer of MPs from a detergent environment to an APol
one, 253
Transfer of MPs from APols to other environments,
306–310
APol exchange, 306
overview of methods, 307
transfer to cells in culture, 308
transfer to detergents, 308
transfer to lipid black films, 308
transfer to lipidic mesophases, 310
transfer to lipid vesicles, 308
transfer to nanodiscs, 310
transfer to SMALPs, 310
transfer to vacuum, 310
Transferring MPs from detergent to APols, 252–259
overview of methods of transfer, 252
Transient receptor potential (TRP) channels, 554
Translational diffusion coefficient (D t )
measurement by QENS, 189, 191, 430
Translocon, 48, 49
Transmembrane α-helices, 18
Transmembrane α-helix bundles, 18
Transmembrane β-barrels, 18
Transverse relaxation time (T 2 ), 454
TRAP1, 295
Trapping MPs in APols
overview of methods, 252
Tribet, C., 153, 264, 382, 416, 467
Tripod amphiphiles, 82
Tris(hydroxymethyl)acrylamidomethane (THAM)
chemical structure, 166
Tritons, 62
Triton X-100
chemical structure, 61
CMC, 61
detergency, 135
Triton X-114, 64
TRPA1, 571, 579
SP cryo-EM reconstruction in PMAL-C8, 571
SP NS EM study in A8-35, 555
stability in SAPol vs. A8-35, 555
TRPM1
SP cryo-EM reconstruction in A8-35, 569
TRPV1, 294, 576, 579
particle orientation in DDM-solubilized vs.
A8-35-trapped SP NS samples, 576
SP cryo-EM reconstruction in A8-35, 564, 566–568
SP cryo-EM reconstruction in A8-35 vs. NDs,
557, 566
TRPV2, 556
aggregation in SAPols and not in A8-35, 556
cryo-EM resolution achieved in various
surfactants, 556
SP cryo-EM reconstruction in A8-35, 569
stability in A8-35 vs. DDM, 556
Tweens, 62
Type A endothelin receptor (ET A )
trapped in APG, 164
Tyrosine kinase
activity in SMALPs, 289
U
Ultracentrifugation
principle, 411
Unwin, N., 13, 39
V
Vaccines
adjuvants, 661
against C. trachomatis, 662
modes of preparation, 661
MP-based subunit vaccines, 669
potential of APol-based vaccines, 664–672
subunit vaccines, 661
use of purified MPs, 661
Vapor diffusion, 503
Vasopressin V2R receptor, 288
Vesicle-associated membrane protein 2 (VAMP-2)
ND-trapped, interaction with SNAREs, 116, 117
Voltage-dependent anion channel-1 (VDAC1)
crystallization from bicelles, 104
3D structure, 19
Voltage-sensitive Na
+ -specific channels, 8
W
Wagner, G., 483
Warschawski, D.E., 103, 458
Watkinson, T.G., 654
White, S.H., 458
X
Xanthorhodopsin
crystallization from bicelles, 104
X-FEL, see Free-electron laser X-ray
X-ray and neutron scattering, 428
Y
YidC, 260
Z
Zaccai, G., 35, 431
Zebrafish STRA6 retinol receptor/calmodulin
complex, 280
Zhang, S., 121
Zito, F., 376
ZMPSTE24 protease, 21
Zoonens, M., 261, 291, 314, 318, 356, 465, 619
Zwittergent 3-14 (Z3-14)
chemical structure, 61
CMC, 61
708
Index
Torpedo marmorata, 288
post-synaptic membrane fragments, 39
as source of nAChR, 39
Toyoshima, C., 47
Transducin
interaction with ND-trapped monomeric
rhodopsin, 116
Transfer of MPs from a detergent environment to an APol
one, 253
Transfer of MPs from APols to other environments,
306–310
APol exchange, 306
overview of methods, 307
transfer to cells in culture, 308
transfer to detergents, 308
transfer to lipid black films, 308
transfer to lipidic mesophases, 310
transfer to lipid vesicles, 308
transfer to nanodiscs, 310
transfer to SMALPs, 310
transfer to vacuum, 310
Transferring MPs from detergent to APols, 252–259
overview of methods of transfer, 252
Transient receptor potential (TRP) channels, 554
Translational diffusion coefficient (D t )
measurement by QENS, 189, 191, 430
Translocon, 48, 49
Transmembrane α-helices, 18
Transmembrane α-helix bundles, 18
Transmembrane β-barrels, 18
Transverse relaxation time (T 2 ), 454
TRAP1, 295
Trapping MPs in APols
overview of methods, 252
Tribet, C., 153, 264, 382, 416, 467
Tripod amphiphiles, 82
Tris(hydroxymethyl)acrylamidomethane (THAM)
chemical structure, 166
Tritons, 62
Triton X-100
chemical structure, 61
CMC, 61
detergency, 135
Triton X-114, 64
TRPA1, 571, 579
SP cryo-EM reconstruction in PMAL-C8, 571
SP NS EM study in A8-35, 555
stability in SAPol vs. A8-35, 555
TRPM1
SP cryo-EM reconstruction in A8-35, 569
TRPV1, 294, 576, 579
particle orientation in DDM-solubilized vs.
A8-35-trapped SP NS samples, 576
SP cryo-EM reconstruction in A8-35, 564, 566–568
SP cryo-EM reconstruction in A8-35 vs. NDs,
557, 566
TRPV2, 556
aggregation in SAPols and not in A8-35, 556
cryo-EM resolution achieved in various
surfactants, 556
SP cryo-EM reconstruction in A8-35, 569
stability in A8-35 vs. DDM, 556
Tweens, 62
Type A endothelin receptor (ET A )
trapped in APG, 164
Tyrosine kinase
activity in SMALPs, 289
U
Ultracentrifugation
principle, 411
Unwin, N., 13, 39
V
Vaccines
adjuvants, 661
against C. trachomatis, 662
modes of preparation, 661
MP-based subunit vaccines, 669
potential of APol-based vaccines, 664–672
subunit vaccines, 661
use of purified MPs, 661
Vapor diffusion, 503
Vasopressin V2R receptor, 288
Vesicle-associated membrane protein 2 (VAMP-2)
ND-trapped, interaction with SNAREs, 116, 117
Voltage-dependent anion channel-1 (VDAC1)
crystallization from bicelles, 104
3D structure, 19
Voltage-sensitive Na
+ -specific channels, 8
W
Wagner, G., 483
Warschawski, D.E., 103, 458
Watkinson, T.G., 654
White, S.H., 458
X
Xanthorhodopsin
crystallization from bicelles, 104
X-FEL, see Free-electron laser X-ray
X-ray and neutron scattering, 428
Y
YidC, 260
Z
Zaccai, G., 35, 431
Zebrafish STRA6 retinol receptor/calmodulin
complex, 280
Zhang, S., 121
Zito, F., 376
ZMPSTE24 protease, 21
Zoonens, M., 261, 291, 314, 318, 356, 465, 619
Zwittergent 3-14 (Z3-14)
chemical structure, 61
CMC, 61
708
Index
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