Moyle, P.M., Toth, I. (2013) Modern subunit vaccines: development, components, and research opportunities.
ChemMedChem 8:360–376.
Nieba, L., Nieba-Axmann, S.E., Persson, A., Hämäläinen, M., Edebratt, F., Hansson, A., Lidholm, J., Magnusson, K.,
Frostell Karlsson, Å., Plückthun, A. (1997) BIACORE analysis of histidine-tagged proteins using a chelating NTA
sensor chip. Anal. Biochem. 252:217–228.
Niemeyer, C.M., Burger, W., Hoedemakers, R.M.J. (1998) Hybridization characteristics of biomolecular adaptors,
covalent DNA-streptavidin conjugates. Bioconjugate Chem. 9:168–175.
Nirthanan, S., Gwee, M.C.E. (2004) Three-finger α-neurotoxins and the nicotinic acetylcholine receptor, forty years
on. J. Pharmacol. Sci. 94:1–17.
Nowaczyk, M., Oworah-Nkruma, R., Zoonens, M., Rögner, M., Popot, J.-L. (2004) Amphipols: strategies for an
improved PS2 environment in aqueous solution, in: Miyake, J., ed., Biohydrogen III. Elsevier, Dordrecht, The
Netherlands, Kyoto, Japan, pp. 151–159.
Ostermeier, C., Iwata, S., Ludwig, B., Michel, H. (1995) F v fragment-mediated crystallization of the membrane protein
bacterial cytochrome c oxidase. Nature Struct. Biol. 2:842–846.
Park, S., Kang, Y.J., Majd, S. (2015) A review of patterned organic bioelectronic materials and their biomedical
applications. Adv. Mater. 27:7583–7619.
Patel, J.D., O’Carra, R., Jones, J., Woodward, J.G., Mumper, R.J. (2007) Preparation and characterization of nickel
nanoparticles for binding to his-tag proteins and antigens. Pharm. Res. 24:343–352.
Pautsch, A., Schulz, G.E. (2000) High-resolution structure of the OmpA membrane domain. J. Mol. Biol. 298:273–282.
Pérez-Mitta, G., Burr, L., Tuninetti, J.S., Trautmann, C., Toimil-Molares, M.E., Azzaroni, O. (2016) Noncovalent
functionalization of solid-state nanopores via self-assembly of amphipols. Nanoscale 8:1470–1478.
Perez, J.B., Martinez, K.L., Segura, J.M., Vogel, H. (2006) Supported cell-membrane sheets for functional fluorescence
imaging of membrane proteins. Adv. Funct. Mater. 16:306–312.
Perlmutter, J.D., Popot, J.-L., Sachs, J.N. (2014) Molecular dynamics simulations of a membrane protein/amphipol
complex. J. Membr. Biol. 247:883–895.
Perry, T., Souabni, H., Rapisarda, C., Fronzes, R., Giusti, F., Popot, J.-L., Zoonens, M., Gubellini, F. (2018) Visualizing
transmembrane regions of protein complexes by electron microscopy using biotinylated amphipols, submitted for
publication.
Pervushin, K., Braun, D., Fernàndez, C., Wüthrich, K. (2000) [
15
N,
1
H]/[
13
C,
1
H]-TROSY for simultaneous detection of
backbone
15
N1
H, aromatic
13
C1
H and side-chain
15
N1
H 2 correlations in large proteins. J. Biomol. NMR
17:195–202.
Petrlova, J., Masarik, M., Potesil, D., Adam, V., Trnkova, L., Kizek, R. (2007) Zeptomole detection of streptavidin using
carbon paste electrode and square-wave voltammetry. Electroanalysis 19:1177–1182.
Pierre, Y., Breyton, C., Kramer, D., Popot, J.-L. (1995) Purification and characterization of the cytochrome b 6 f complex
from Chlamydomonas reinhardtii. J. Biol. Chem. 270:29342–29349.
Polovinkin, V., Balandin, T., Volkov, O., Round, E., Borshchevskiy, V., Utrobin, P., von Stetten, D., Royant, A.,
Willbold, D., Arzumanyan, A., Popot, J.-L., Gordeliy, V. (2014) Nanoparticle surface-enhanced Raman scattering of
bacteriorhodopsin stabilized by amphipol A8-35. J. Membr. Biol. 247:971–980.
Ravi, V., Binz, J.M., Rioux, R.M. (2013) Thermodynamic profiles at the solvated inorganicÀorganic interface: The case
of gold-thiolate monolayers. Nano Lett. 13:4442–4448.
Rich, R.L., Myszka, D.G. (2005) Survey of the year 2004 commercial optical biosensor literature. J. Mol. Recognit.
18:431–478.
Schmid, E.L., Tairi, A.P., Hovius, R., Vogel, H. (1998) Screening ligands for membrane protein receptors by total
internal reflection fluorescence: The 5-HT 3 serotonin receptor. Anal. Chem. 70:1331–1338.
Seeger, M.A., Zbinden, R., Flutsch, A., Gutte, P., Engeler, S., Roschitzki-Voser, H., Grütter, M.G. (2013) Design,
construction, and characterization of a second-generation DARPin library with reduced hydrophobicity. Prot. Sci.
22:1239–1257.
Selmi, D.N., Adamson, R.J., Attrill, H., Goddard, A.D., Gilbert, R.J.C., Watts, A., Turberfield, A.J. (2011)
DNA-templated protein arrays for single-molecule imaging. Nano Lett. 11:657–660.
Sennhauser, G., Grütter, M.G. (2008) Chaperone-assisted crystallography with DARPins. Structure 16:1443–1453.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Skerra, A. (2007) Alternative non-antibody scaffolds for molecular recognition. Curr. Opin. Biotechnol. 18:295–304.
Stenberg, E., Persson, B., Roos, H., Urbaniczky, C. (1991) Quantitative determination of surface concentration of protein
with surface plasmon resonance using radio-labeled proteins. J. Colloid Interface Sci. 143:513–526.
Stroebel, D., Choquet, Y., Popot, J.-L., Picot, D. (2003) An atypical haem in the cytochrome b 6 f complex. Nature
426:413–418.
622
13 Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications
ChemMedChem 8:360–376.
Nieba, L., Nieba-Axmann, S.E., Persson, A., Hämäläinen, M., Edebratt, F., Hansson, A., Lidholm, J., Magnusson, K.,
Frostell Karlsson, Å., Plückthun, A. (1997) BIACORE analysis of histidine-tagged proteins using a chelating NTA
sensor chip. Anal. Biochem. 252:217–228.
Niemeyer, C.M., Burger, W., Hoedemakers, R.M.J. (1998) Hybridization characteristics of biomolecular adaptors,
covalent DNA-streptavidin conjugates. Bioconjugate Chem. 9:168–175.
Nirthanan, S., Gwee, M.C.E. (2004) Three-finger α-neurotoxins and the nicotinic acetylcholine receptor, forty years
on. J. Pharmacol. Sci. 94:1–17.
Nowaczyk, M., Oworah-Nkruma, R., Zoonens, M., Rögner, M., Popot, J.-L. (2004) Amphipols: strategies for an
improved PS2 environment in aqueous solution, in: Miyake, J., ed., Biohydrogen III. Elsevier, Dordrecht, The
Netherlands, Kyoto, Japan, pp. 151–159.
Ostermeier, C., Iwata, S., Ludwig, B., Michel, H. (1995) F v fragment-mediated crystallization of the membrane protein
bacterial cytochrome c oxidase. Nature Struct. Biol. 2:842–846.
Park, S., Kang, Y.J., Majd, S. (2015) A review of patterned organic bioelectronic materials and their biomedical
applications. Adv. Mater. 27:7583–7619.
Patel, J.D., O’Carra, R., Jones, J., Woodward, J.G., Mumper, R.J. (2007) Preparation and characterization of nickel
nanoparticles for binding to his-tag proteins and antigens. Pharm. Res. 24:343–352.
Pautsch, A., Schulz, G.E. (2000) High-resolution structure of the OmpA membrane domain. J. Mol. Biol. 298:273–282.
Pérez-Mitta, G., Burr, L., Tuninetti, J.S., Trautmann, C., Toimil-Molares, M.E., Azzaroni, O. (2016) Noncovalent
functionalization of solid-state nanopores via self-assembly of amphipols. Nanoscale 8:1470–1478.
Perez, J.B., Martinez, K.L., Segura, J.M., Vogel, H. (2006) Supported cell-membrane sheets for functional fluorescence
imaging of membrane proteins. Adv. Funct. Mater. 16:306–312.
Perlmutter, J.D., Popot, J.-L., Sachs, J.N. (2014) Molecular dynamics simulations of a membrane protein/amphipol
complex. J. Membr. Biol. 247:883–895.
Perry, T., Souabni, H., Rapisarda, C., Fronzes, R., Giusti, F., Popot, J.-L., Zoonens, M., Gubellini, F. (2018) Visualizing
transmembrane regions of protein complexes by electron microscopy using biotinylated amphipols, submitted for
publication.
Pervushin, K., Braun, D., Fernàndez, C., Wüthrich, K. (2000) [
15
N,
1
H]/[
13
C,
1
H]-TROSY for simultaneous detection of
backbone
15
N1
H, aromatic
13
C1
H and side-chain
15
N1
H 2 correlations in large proteins. J. Biomol. NMR
17:195–202.
Petrlova, J., Masarik, M., Potesil, D., Adam, V., Trnkova, L., Kizek, R. (2007) Zeptomole detection of streptavidin using
carbon paste electrode and square-wave voltammetry. Electroanalysis 19:1177–1182.
Pierre, Y., Breyton, C., Kramer, D., Popot, J.-L. (1995) Purification and characterization of the cytochrome b 6 f complex
from Chlamydomonas reinhardtii. J. Biol. Chem. 270:29342–29349.
Polovinkin, V., Balandin, T., Volkov, O., Round, E., Borshchevskiy, V., Utrobin, P., von Stetten, D., Royant, A.,
Willbold, D., Arzumanyan, A., Popot, J.-L., Gordeliy, V. (2014) Nanoparticle surface-enhanced Raman scattering of
bacteriorhodopsin stabilized by amphipol A8-35. J. Membr. Biol. 247:971–980.
Ravi, V., Binz, J.M., Rioux, R.M. (2013) Thermodynamic profiles at the solvated inorganicÀorganic interface: The case
of gold-thiolate monolayers. Nano Lett. 13:4442–4448.
Rich, R.L., Myszka, D.G. (2005) Survey of the year 2004 commercial optical biosensor literature. J. Mol. Recognit.
18:431–478.
Schmid, E.L., Tairi, A.P., Hovius, R., Vogel, H. (1998) Screening ligands for membrane protein receptors by total
internal reflection fluorescence: The 5-HT 3 serotonin receptor. Anal. Chem. 70:1331–1338.
Seeger, M.A., Zbinden, R., Flutsch, A., Gutte, P., Engeler, S., Roschitzki-Voser, H., Grütter, M.G. (2013) Design,
construction, and characterization of a second-generation DARPin library with reduced hydrophobicity. Prot. Sci.
22:1239–1257.
Selmi, D.N., Adamson, R.J., Attrill, H., Goddard, A.D., Gilbert, R.J.C., Watts, A., Turberfield, A.J. (2011)
DNA-templated protein arrays for single-molecule imaging. Nano Lett. 11:657–660.
Sennhauser, G., Grütter, M.G. (2008) Chaperone-assisted crystallography with DARPins. Structure 16:1443–1453.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Skerra, A. (2007) Alternative non-antibody scaffolds for molecular recognition. Curr. Opin. Biotechnol. 18:295–304.
Stenberg, E., Persson, B., Roos, H., Urbaniczky, C. (1991) Quantitative determination of surface concentration of protein
with surface plasmon resonance using radio-labeled proteins. J. Colloid Interface Sci. 143:513–526.
Stroebel, D., Choquet, Y., Popot, J.-L., Picot, D. (2003) An atypical haem in the cytochrome b 6 f complex. Nature
426:413–418.
622
13 Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications