Davis, K.M., Fleckenstein, H., Galli, L., Hau-Riege, S.P., Kassemeyer, S., Laksmono, H., Liang, M., Lomb, L.,
Marchesini, S., Martin, A.V., Messerschmidt, M., Milathianaki, D., Nass, K., Ros, A., Roy-Chowdhury, S., Schmidt,
K., Seibert, M., Steinbrener, J., Stellato, F., Yan, L., Yoon, C., Moore, T.A., Moore, A.L., Pushkar, Y., Williams, G.
J., Boutet, S., Doak, R.B., Weierstall, U., Frank, M., Chapman, H.N., Spence, J.C., Fromme, P. (2014) Serial timeresolved crystallography of photosystem II using a femtosecond X-ray laser. Nature 513:261–265.
Landau, E.M., Rosenbusch, J.P. (1996) Lipid cubic phases: A novel concept for the crystallization of membrane proteins.
Proc. Natl. Acad. Sci. USA 93:14532–14535.
Lanyi, J.K. (2004) X-ray diffraction of bacteriorhodopsin photocycle intermediates. Mol. Membr. Biol. 21:143–150.
Le Bon, C., Popot, J.-L., Giusti, F. (2014) Labeling and functionalizing amphipols for biological applications. J. Membr.
Biol. 247:797–814.
Li, D., Lee, J., Caffrey, M. (2011) Crystallizing membrane proteins in lipidic mesophases. A host lipid screen. Cryst.
Growth Des. 11:530–537.
Li, D., Shah, S.T.A., Caffrey, M. (2013) Host lipid and temperature as important screening variables for crystallizing
integral membrane proteins in lipidic mesophases. Trials with diacylglycerol kinase. Cryst. Growth Des.
13:2846–2857.
Liao, M., Cao, E., Julius, D., Cheng, Y. (2013) Structure of the TRPV1 ion channel determined by electron cryomicroscopy. Nature 504:107–112.
Liao, M., Cao, E., Julius, D., Cheng, Y. (2014) Single particle electron cryo-microscopy of a mammalian ion channel.
Curr. Opin. Struct. Biol. 27:1–7.
Lindblom, G., Rilfors, L. (1989) Cubic phases and isotropic structures formed by membrane lipids–possible biological
relevance. Biochim. Biophys. Acta 988:221–256.
Liu, Z., Yan, H., Wang, K., Kuang, T., Zhang, J., Gui, L., An, X., Chang, W. (2004) Crystal structure of spinach major
light-harvesting complex at 2.72 Å resolution. Nature 428:287–291.
Lluis, M.W., Godfroy, J.I., III, Yin, H. (2013) Protein engineering methods applied to membrane protein targets. Prot.
Eng. Des. Sel. 26:91–100.
Loll, P.J. (2014) Membrane proteins, detergents and crystals: what is the state of the art? Acta Crystallogr. F
70:1576–1583.
Love, J., Mancia, F., Shapiro, L., Punta, M., Rost, B., Girvin, M., Wang, D.N., Zhou, M., Hunt, J.F., Szyperski, T.,
Gouaux, E., Mackinnon, R., McDermott, A., Honig, B., Inouye, M., Montelione, G., Hendrickson, W.A. (2010) The
New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural
genomics of integral membrane proteins. J. Struct. Funct. Genomics 11:191–199.
Luecke, H., Schobert, B., Richter, H.-T., Cartailler, J.-P., Lanyi, J.K. (1999) Structure of bacteriorhodopsin at 1.55 Å
resolution. J. Mol. Biol. 291: 899–911.
Luecke, H., Schobert, B., Stagno, J., Imasheva, E.S., Wang, J.M., Balashov, S.P., Lanyi, J.K. (2008) Crystallographic
structure of xanthorhodopsin, the light-driven proton pump with a dual chromophore. Proc. Natl. Acad. Sci. USA
105:16561–16565.
Magnani, F., Shibata, Y., Serrano-Vega, M.J., Tate, C.G. (2008) Co-evolving stability and conformational homogeneity
of the human adenosine A 2a receptor. Proc. Natl. Acad. Sci. USA 105:10744–10749.
Martin-Garcia, J.M., Conrad, C.E., Coe, J., Roy-Chowdhury, S., Fromme, P. (2016) Serial femtosecond crystallography:
A revolution in structural biology. Arch. Biochem. Biophys. 602:32–47.
Michel, H. (1982) Three-dimensional crystals of a membrane protein complex. The photosynthetic reaction centre from
Rhodopseudomonas viridis. J. Mol. Biol. 158:567–572.
Michel, H. (1983) Crystallization of membrane proteins. Trends Biochem. Sci. 8:56–59.
Michel, H. (1991) Crystallization of Membrane Proteins. First ed. CRC Press, Boca Raton, 224 p.
Michel, H., Epp, O., Deisenhofer, J. (1986) Pigment-protein interactions in the photosynthetic reaction centre from
Rhodopseudomonas viridis. EMBO J. 5:2445–2451.
Michel, H., Oesterhelt, D. (1980) Three-dimensional crystals of membrane proteins: bacteriorhodopsin. Proc. Natl.
Acad. Sci. USA 77:1283–1285.
Miller, J.L., Tate, C.G. (2011) Engineering an ultra-thermostable β 1 -adrenoceptor. J. Mol. Biol. 413:628–638.
Moraes, I., Gwyndaf Evans, G., Sanchez-Weatherby, J., Newstead, S., Stewart, P.D.S. (2014) Membrane protein
structure determination–The next generation. Biochim. Biophys. Acta 1838:78–87.
Nagy, J.K., Kuhn Hoffmann, A., Keyes, M.H., Gray, D.N., Oxenoid, K., Sanders, C.R. (2001) Use of amphipathic
polymers to deliver a membrane protein to lipid bilayers. FEBS Lett. 501:115–120.
Neutze, R., Brändén, G., Schertler, G.F. (2015) Membrane protein structural biology using X-ray free electron lasers.
Curr. Opin. Struct. Biol. 33:115–125.
Neutze, R., Remco, W., van der Spoel, D., Weckert, E., Hajdu, J. (2000) Potential for biomolecular imaging with
femtosecond X-ray pulses. Nature 406:752–757.
528
11 Amphipols and Membrane Protein Crystallization
Marchesini, S., Martin, A.V., Messerschmidt, M., Milathianaki, D., Nass, K., Ros, A., Roy-Chowdhury, S., Schmidt,
K., Seibert, M., Steinbrener, J., Stellato, F., Yan, L., Yoon, C., Moore, T.A., Moore, A.L., Pushkar, Y., Williams, G.
J., Boutet, S., Doak, R.B., Weierstall, U., Frank, M., Chapman, H.N., Spence, J.C., Fromme, P. (2014) Serial timeresolved crystallography of photosystem II using a femtosecond X-ray laser. Nature 513:261–265.
Landau, E.M., Rosenbusch, J.P. (1996) Lipid cubic phases: A novel concept for the crystallization of membrane proteins.
Proc. Natl. Acad. Sci. USA 93:14532–14535.
Lanyi, J.K. (2004) X-ray diffraction of bacteriorhodopsin photocycle intermediates. Mol. Membr. Biol. 21:143–150.
Le Bon, C., Popot, J.-L., Giusti, F. (2014) Labeling and functionalizing amphipols for biological applications. J. Membr.
Biol. 247:797–814.
Li, D., Lee, J., Caffrey, M. (2011) Crystallizing membrane proteins in lipidic mesophases. A host lipid screen. Cryst.
Growth Des. 11:530–537.
Li, D., Shah, S.T.A., Caffrey, M. (2013) Host lipid and temperature as important screening variables for crystallizing
integral membrane proteins in lipidic mesophases. Trials with diacylglycerol kinase. Cryst. Growth Des.
13:2846–2857.
Liao, M., Cao, E., Julius, D., Cheng, Y. (2013) Structure of the TRPV1 ion channel determined by electron cryomicroscopy. Nature 504:107–112.
Liao, M., Cao, E., Julius, D., Cheng, Y. (2014) Single particle electron cryo-microscopy of a mammalian ion channel.
Curr. Opin. Struct. Biol. 27:1–7.
Lindblom, G., Rilfors, L. (1989) Cubic phases and isotropic structures formed by membrane lipids–possible biological
relevance. Biochim. Biophys. Acta 988:221–256.
Liu, Z., Yan, H., Wang, K., Kuang, T., Zhang, J., Gui, L., An, X., Chang, W. (2004) Crystal structure of spinach major
light-harvesting complex at 2.72 Å resolution. Nature 428:287–291.
Lluis, M.W., Godfroy, J.I., III, Yin, H. (2013) Protein engineering methods applied to membrane protein targets. Prot.
Eng. Des. Sel. 26:91–100.
Loll, P.J. (2014) Membrane proteins, detergents and crystals: what is the state of the art? Acta Crystallogr. F
70:1576–1583.
Love, J., Mancia, F., Shapiro, L., Punta, M., Rost, B., Girvin, M., Wang, D.N., Zhou, M., Hunt, J.F., Szyperski, T.,
Gouaux, E., Mackinnon, R., McDermott, A., Honig, B., Inouye, M., Montelione, G., Hendrickson, W.A. (2010) The
New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural
genomics of integral membrane proteins. J. Struct. Funct. Genomics 11:191–199.
Luecke, H., Schobert, B., Richter, H.-T., Cartailler, J.-P., Lanyi, J.K. (1999) Structure of bacteriorhodopsin at 1.55 Å
resolution. J. Mol. Biol. 291: 899–911.
Luecke, H., Schobert, B., Stagno, J., Imasheva, E.S., Wang, J.M., Balashov, S.P., Lanyi, J.K. (2008) Crystallographic
structure of xanthorhodopsin, the light-driven proton pump with a dual chromophore. Proc. Natl. Acad. Sci. USA
105:16561–16565.
Magnani, F., Shibata, Y., Serrano-Vega, M.J., Tate, C.G. (2008) Co-evolving stability and conformational homogeneity
of the human adenosine A 2a receptor. Proc. Natl. Acad. Sci. USA 105:10744–10749.
Martin-Garcia, J.M., Conrad, C.E., Coe, J., Roy-Chowdhury, S., Fromme, P. (2016) Serial femtosecond crystallography:
A revolution in structural biology. Arch. Biochem. Biophys. 602:32–47.
Michel, H. (1982) Three-dimensional crystals of a membrane protein complex. The photosynthetic reaction centre from
Rhodopseudomonas viridis. J. Mol. Biol. 158:567–572.
Michel, H. (1983) Crystallization of membrane proteins. Trends Biochem. Sci. 8:56–59.
Michel, H. (1991) Crystallization of Membrane Proteins. First ed. CRC Press, Boca Raton, 224 p.
Michel, H., Epp, O., Deisenhofer, J. (1986) Pigment-protein interactions in the photosynthetic reaction centre from
Rhodopseudomonas viridis. EMBO J. 5:2445–2451.
Michel, H., Oesterhelt, D. (1980) Three-dimensional crystals of membrane proteins: bacteriorhodopsin. Proc. Natl.
Acad. Sci. USA 77:1283–1285.
Miller, J.L., Tate, C.G. (2011) Engineering an ultra-thermostable β 1 -adrenoceptor. J. Mol. Biol. 413:628–638.
Moraes, I., Gwyndaf Evans, G., Sanchez-Weatherby, J., Newstead, S., Stewart, P.D.S. (2014) Membrane protein
structure determination–The next generation. Biochim. Biophys. Acta 1838:78–87.
Nagy, J.K., Kuhn Hoffmann, A., Keyes, M.H., Gray, D.N., Oxenoid, K., Sanders, C.R. (2001) Use of amphipathic
polymers to deliver a membrane protein to lipid bilayers. FEBS Lett. 501:115–120.
Neutze, R., Brändén, G., Schertler, G.F. (2015) Membrane protein structural biology using X-ray free electron lasers.
Curr. Opin. Struct. Biol. 33:115–125.
Neutze, R., Remco, W., van der Spoel, D., Weckert, E., Hajdu, J. (2000) Potential for biomolecular imaging with
femtosecond X-ray pulses. Nature 406:752–757.
528
11 Amphipols and Membrane Protein Crystallization