Barthelmess, M., Caleman, C., Boutet, S., Bogan, M.J., Krzywinski, J., Bostedt, C., Bajt, S., Gumprecht, L., Rudek,
B., Erk, B., Schmidt, C., Hömke, A., Reich, C., Pietschner, D., Strüder, L., Hauser, G., Gorke, H., Ullrich, J.,
Herrmann, S., Schaller, G., Schopper, F., Soltau, H., Kühnel, K.-U., Messerschmidt, M., Bozek, J.D., Hau-Riege, S.
P., Frank, M., Hampton, C.Y., Sierra, R.G., Starodub, D., Williams, G.J., Hajdu, J., Timneanu, N., Seibert, M.M.,
Andreasson, J., Rocker, A., Jönsson, O., Svenda, M., Stern, S., Nass, K., Andritschke, R., Schröter, C.-D., Krasniqi,
F., Bott, M., Schmidt, K.E., Wang, X.-Y., Grotjohann, I., Holton, J.M., Barends, T.R.M., Neutze, R., Marchesini, S.,
Fromme, R., Schorb, S., Rupp, D., Adolph, M., Gorkhover, T., Andersson, I., Hirsemann, H., Potdevin, G.,
Graafsma, H., Nilsson, B., Spence, J.C.H. (2011) Femtosecond X-ray protein nanocrystallography. Nature
470:73–77.
Charvolin, D., Picard, M., Huang, L.-S., Berry, E.A., Popot, J.-L. (2014) Solution behavior and crystallization of
cytochrome bc 1 in the presence of amphipols. J. Membr. Biol. 247:981–996.
Cherezov, V. (2011) Lipidic cubic phase technologies for membrane protein structural studies. Curr. Opin. Struct. Biol.
21:559–566.
Cherezov, V., J. C, Papiz, M.Z., Caffrey, M. (2006) Room to move: crystallizing membrane proteins in swollen lipidic
mesophases. J. Mol. Biol. 357:1605–1618.
Cherezov, V., Rosenbaum, D.M., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Kuhn, P.,
Weis, W.I., Kobilka, B.K., Stevens, R.C. (2007) High-resolution crystal structure of an engineered human β 2 -
adrenergic G protein-coupled receptor. Science 318:1258–1265.
Cowan, S.W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauptit, R.A., Jansonius, J.N., Rosenbusch, J.P. (1992)
Crystal structures explain functional properties of two E. coli porins. Nature 358:727–733.
Cuesta-Seijo, J.A., Neale, C., Khan, M.A., Moktar, J., Tran, C.D., Bishop, R.E., Pomès, R., Privé, G.G. (2010) PagP
crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler. Structure
18:1210–1219.
Dahmane, T., Damian, M., Mary, S., Popot, J.-L., Banères, J.-L. (2009) Amphipol-assisted in vitro folding of G proteincoupled receptors. Biochemistry 48:6516–6521.
Dahmane, T., Rappaport, F., Popot, J.-L. (2013) Amphipol-assisted folding of bacteriorhodopsin in the presence and
absence of lipids. Functional consequences. Eur. Biophys. J. 42:85–101.
Deisenhofer, J., Epp, O., Miki, K., Huber, R., Michel, H. (1984) X-ray structure analysis of a membrane protein complex.
Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center from
Rhodopseudomonas viridis. J. Mol. Biol. 180:385–398.
Deisenhofer, J., Epp, O., Miki, K., Huber, R., Michel, H. (1985) Structure of the protein subunits in the photosynthetic
reaction center of Rhodopseudomonas viridis at 3 Å resolution. Nature 318:618–624.
Deisenhofer, J., Michel, H. (1989) The photosynthetic reaction center from the purple bacterium Rhodopseudomonas
viridis. EMBO J. 8:2149–2170.
Diab, C., Tribet, C., Gohon, Y., Popot, J.-L., Winnik, F.M. (2007a) Complexation of integral membrane proteins by
phosphorylcholine-based amphipols. Biochim. Biophys. Acta 1768:2737–2747.
Diab, C., Winnik, F.M., Tribet, C. (2007b) Enthalpy of interaction and binding isotherms of non-ionic surfactants onto
micellar amphiphilic polymers (amphipols). Langmuir 23:3025–3035.
Egloff, P., Hillenbrand, M., Klenk, C., Batyuk, A., Heine, P., Balada, S., Schlinkmann, K.M., Scott, D.J., Schütz, M.,
Plückthun, A. (2014) Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in
Escherichia coli. Proc. Natl. Acad. Sci. USA 111:E655–E662.
Faham, S., Bowie, J.U. (2002) Bicelle crystallization: a new method for crystallizing membrane proteins yields a
monomeric bacteriorhodopsin structure. J. Mol. Biol. 316:1–6.
Ferrandez, Y., Dezi, M., Bosco, M., Urvoas, A., Valério, M., Le Bon, C., Giusti, F., Broutin, I., Durand, G., Polidori, A.,
Popot, J.-L., Picard, M., Minard, P. (2014) Amphipol-mediated screening of molecular orthoses specific for
membrane protein targets. J. Membr. Biol. 247:925–940.
Garavito, R.M., Jenkins, J., Jansonius, J.N., Karlsson, R., Rosenbusch, J.P. (1983) X-ray diffraction analysis of matrix
porin, an integral membrane protein from Escherichia coli outer membranes. J. Mol. Biol. 164:313–327.
Garavito, R.M., Picot, D., Loll, P.J. (1996) Strategies for crystallizing membrane proteins. J. Bioenerg. Biomembr.
28:13–27.
Garavito, R.M., Rosenbusch, J.P. (1980) Three-dimensional crystals of an integral membrane protein: an initial X-ray
analysis. J. Cell Biol. 86:327–329.
Giusti, F., Rieger, J., Catoire, L., Qian, S., Calabrese, A.N., Watkinson, T.G., Casiraghi, M., Radford, S.E., Ashcroft, A.
E., Popot, J.-L. (2014) Synthesis, characterization and applications of a perdeuterated amphipol. J. Membr. Biol.
247:909–924.
Gohon, Y., Dahmane, T., Ruigrok, R., Schuck, P., Charvolin, D., Rappaport, F., Timmins, P., Engelman, D.M., Tribet,
C., Popot, J.-L., Ebel, C. (2008) Bacteriorhodopsin/amphipol complexes: structural and functional properties.
Biophys. J. 94:3523–3537.
526
11 Amphipols and Membrane Protein Crystallization
B., Erk, B., Schmidt, C., Hömke, A., Reich, C., Pietschner, D., Strüder, L., Hauser, G., Gorke, H., Ullrich, J.,
Herrmann, S., Schaller, G., Schopper, F., Soltau, H., Kühnel, K.-U., Messerschmidt, M., Bozek, J.D., Hau-Riege, S.
P., Frank, M., Hampton, C.Y., Sierra, R.G., Starodub, D., Williams, G.J., Hajdu, J., Timneanu, N., Seibert, M.M.,
Andreasson, J., Rocker, A., Jönsson, O., Svenda, M., Stern, S., Nass, K., Andritschke, R., Schröter, C.-D., Krasniqi,
F., Bott, M., Schmidt, K.E., Wang, X.-Y., Grotjohann, I., Holton, J.M., Barends, T.R.M., Neutze, R., Marchesini, S.,
Fromme, R., Schorb, S., Rupp, D., Adolph, M., Gorkhover, T., Andersson, I., Hirsemann, H., Potdevin, G.,
Graafsma, H., Nilsson, B., Spence, J.C.H. (2011) Femtosecond X-ray protein nanocrystallography. Nature
470:73–77.
Charvolin, D., Picard, M., Huang, L.-S., Berry, E.A., Popot, J.-L. (2014) Solution behavior and crystallization of
cytochrome bc 1 in the presence of amphipols. J. Membr. Biol. 247:981–996.
Cherezov, V. (2011) Lipidic cubic phase technologies for membrane protein structural studies. Curr. Opin. Struct. Biol.
21:559–566.
Cherezov, V., J. C, Papiz, M.Z., Caffrey, M. (2006) Room to move: crystallizing membrane proteins in swollen lipidic
mesophases. J. Mol. Biol. 357:1605–1618.
Cherezov, V., Rosenbaum, D.M., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Kuhn, P.,
Weis, W.I., Kobilka, B.K., Stevens, R.C. (2007) High-resolution crystal structure of an engineered human β 2 -
adrenergic G protein-coupled receptor. Science 318:1258–1265.
Cowan, S.W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauptit, R.A., Jansonius, J.N., Rosenbusch, J.P. (1992)
Crystal structures explain functional properties of two E. coli porins. Nature 358:727–733.
Cuesta-Seijo, J.A., Neale, C., Khan, M.A., Moktar, J., Tran, C.D., Bishop, R.E., Pomès, R., Privé, G.G. (2010) PagP
crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler. Structure
18:1210–1219.
Dahmane, T., Damian, M., Mary, S., Popot, J.-L., Banères, J.-L. (2009) Amphipol-assisted in vitro folding of G proteincoupled receptors. Biochemistry 48:6516–6521.
Dahmane, T., Rappaport, F., Popot, J.-L. (2013) Amphipol-assisted folding of bacteriorhodopsin in the presence and
absence of lipids. Functional consequences. Eur. Biophys. J. 42:85–101.
Deisenhofer, J., Epp, O., Miki, K., Huber, R., Michel, H. (1984) X-ray structure analysis of a membrane protein complex.
Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center from
Rhodopseudomonas viridis. J. Mol. Biol. 180:385–398.
Deisenhofer, J., Epp, O., Miki, K., Huber, R., Michel, H. (1985) Structure of the protein subunits in the photosynthetic
reaction center of Rhodopseudomonas viridis at 3 Å resolution. Nature 318:618–624.
Deisenhofer, J., Michel, H. (1989) The photosynthetic reaction center from the purple bacterium Rhodopseudomonas
viridis. EMBO J. 8:2149–2170.
Diab, C., Tribet, C., Gohon, Y., Popot, J.-L., Winnik, F.M. (2007a) Complexation of integral membrane proteins by
phosphorylcholine-based amphipols. Biochim. Biophys. Acta 1768:2737–2747.
Diab, C., Winnik, F.M., Tribet, C. (2007b) Enthalpy of interaction and binding isotherms of non-ionic surfactants onto
micellar amphiphilic polymers (amphipols). Langmuir 23:3025–3035.
Egloff, P., Hillenbrand, M., Klenk, C., Batyuk, A., Heine, P., Balada, S., Schlinkmann, K.M., Scott, D.J., Schütz, M.,
Plückthun, A. (2014) Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in
Escherichia coli. Proc. Natl. Acad. Sci. USA 111:E655–E662.
Faham, S., Bowie, J.U. (2002) Bicelle crystallization: a new method for crystallizing membrane proteins yields a
monomeric bacteriorhodopsin structure. J. Mol. Biol. 316:1–6.
Ferrandez, Y., Dezi, M., Bosco, M., Urvoas, A., Valério, M., Le Bon, C., Giusti, F., Broutin, I., Durand, G., Polidori, A.,
Popot, J.-L., Picard, M., Minard, P. (2014) Amphipol-mediated screening of molecular orthoses specific for
membrane protein targets. J. Membr. Biol. 247:925–940.
Garavito, R.M., Jenkins, J., Jansonius, J.N., Karlsson, R., Rosenbusch, J.P. (1983) X-ray diffraction analysis of matrix
porin, an integral membrane protein from Escherichia coli outer membranes. J. Mol. Biol. 164:313–327.
Garavito, R.M., Picot, D., Loll, P.J. (1996) Strategies for crystallizing membrane proteins. J. Bioenerg. Biomembr.
28:13–27.
Garavito, R.M., Rosenbusch, J.P. (1980) Three-dimensional crystals of an integral membrane protein: an initial X-ray
analysis. J. Cell Biol. 86:327–329.
Giusti, F., Rieger, J., Catoire, L., Qian, S., Calabrese, A.N., Watkinson, T.G., Casiraghi, M., Radford, S.E., Ashcroft, A.
E., Popot, J.-L. (2014) Synthesis, characterization and applications of a perdeuterated amphipol. J. Membr. Biol.
247:909–924.
Gohon, Y., Dahmane, T., Ruigrok, R., Schuck, P., Charvolin, D., Rappaport, F., Timmins, P., Engelman, D.M., Tribet,
C., Popot, J.-L., Ebel, C. (2008) Bacteriorhodopsin/amphipol complexes: structural and functional properties.
Biophys. J. 94:3523–3537.
526
11 Amphipols and Membrane Protein Crystallization