Giusti, F., Kessler, P., Westh Hansen, R., Della Pia, E.A., Le Bon, C., Mourier, G., Popot, J.-L., Martinez, K.L., Zoonens,
M. (2015) Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins. Biomacromolecules 16:3751–3761.
Gohon, Y. (2002) Etude structurale et fonctionnelle de deux protéines membranaires, la bactériorhodopsine et le
récepteur nicotinique de l’acétylcholine, maintenues en solution aqueuse non détergente par des polymères
amphiphiles. Thèse de Doctorat, Université Paris-VI, Paris, 467 p.
Gohon, Y., Pavlov, G., Timmins, P., Tribet, C., Popot, J.-L., Ebel, C. (2004) Partial specific volume and solvent
interactions of amphipol A8-35. Anal. Biochem. 334:318–334.
Gohon, Y., Giusti, F., Prata, C., Charvolin, D., Timmins, P., Ebel, C., Tribet, C., Popot, J.-L. (2006) Well-defined
nanoparticles formed by hydrophobic assembly of a short and polydisperse random terpolymer, amphipol A8-35.
Langmuir 22:1281–1290.
Gohon, Y., Dahmane, T., Ruigrok, R., Schuck, P., Charvolin, D., Rappaport, F., Timmins, P., Engelman, D.M., Tribet,
C., Popot, J.-L., Ebel, C. (2008) Bacteriorhodopsin/amphipol complexes: structural and functional properties.
Biophys. J. 94:3523–3537.
Gohon, Y., Vindigni, J.-D., Pallier, A., Wien, F., Celia, H., Giuliani, A., Tribet, C., Chardot, T., Briozzo, P. (2011) High
water solubility and fold in amphipols of proteins with large hydrophobic regions: Oleosins and caleosin from seed
lipid bodies. Biochim. Biophys. Acta 1808:706–716.
Guinier, A., Fournet, G. (1955) Small-angle scattering of X-rays. Wiley, New York, 268 p.
Gulati, S., Jamshad, M., Knowles, T.J., Morrison, K.A., Downing, R., Cant, N., Collins, R., Koenderink, J.B., Ford, R.
C., Overduin, M., Kerr, I.D., Dafforn, T.R., Rothnie, A.J. (2014) Detergent-free purification of ABC (ATP-bindingcassette) transporters. Biochem. J. 461:269–278.
Han, S.G., Na, J.H., Lee, W.K., Park, D., Oh, J., Yoon, S.H., Lee, C.K., Sung, M.H., Shin, Y.K., Yu, Y.G. (2014) An
amphipathic polypeptide derived from poly-γ-glutamic acid for the stabilization of membrane proteins. Prot. Sci.
23:1800–1807.
Han, S.G., Baek, S.I., Son, T.J., Lee, H., Kim, N.H., Yu, Y.G. (2017) Preparation of functional human lysophosphatidic
acid receptor 2 using a P9* expression system and an amphipathic polymer and investigation of its in vitro binding
preference to G α proteins. Biochem. Biophys. Res. Commun. 487:103–108.
Harlan, J.E., Picot, D., Loll, P.J., Garavito, R.M. (1995) Calibration of size-exclusion chromatography: use of a double
Gaussian distribution to describe pore sizes. Anal. Biochem. 224:557–563.
Hiruma-Shimizu, K., Shimizu, H., Thompson, G.S., Kalverda, A.P., Patching, S.G. (2015) Deuterated detergents for
structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Mol. Membr.
Biol. 32:139–155.
Huynh, K.W., Cohen, M.R., Moiseenkova-Bell, V.Y. (2014) Application of amphipols for structure-functional analysis
of TRP channels. J. Membr. Biol. 247:843–851.
Jacrot, B. (1976) The study of biological structures by neutron scattering from solution. Rep. Prog. Phys. 39:911–953.
Jamshad, M., Charlton, J., Lin, Y.-P., Routledge, S.J., Bawa, Z., Knowles, T.J., Overduin, M., Dekker, N., Dafforn, T.R.,
Bill, R.M., Poyner, D.R., Wheatley, M. (2015) G protein-coupled receptor solubilization and purification for
biophysical analysis and functional studies, in the total absence of detergent. Biosc. Rep. 35:e00188.
Jin, P., Bulkley, D., Guo, Y., Zhang, W., Guo, Z., Huynh, W., Wu, S., Meltzer, S., Cheng, T., Jan, L.Y., Jan, Y.-N.,
Cheng, Y. (2017) Electron cryo-microscopy structure of the mechanotransduction channel NOMPC. Nature
547:118–122.
Klammt, C., Perrin, M.-H., Maslennikov, I., Renault, L., Krupa, M., Kwiatkowski, W., Stahlberg, H., Vale, W., Choe,
S. (2011) Polymer-based cell-free expression of ligand-binding family B G protein-coupled receptors without
detergents. Prot. Sci. 20:1030–1041.
Knowles, T.J., Finka, R., Smith, C., Lin, Y.-P., Dafforn, T., Overduin, M. (2009) Membrane proteins solubilized intact in
lipid containing nanoparticles bounded by styrene maleic acid copolymer. J. Am. Chem. Soc. 131:7484–7485.
Kraft, T.E., Hresko, R.C., Hruz, P.W. (2015) Expression, purification, and functional characterization of the insulinresponsive facilitative glucose transporter GLUT4. Protein Sci. 24:2008–2019.
Laue, T.M., Stafford, W.E., III (1999) Modern application of analytical ultracentrifugation. Annu. Rev. Biophys. Biomol.
Struct. 28:75–100.
Laursen, T., Naur, P., Møller, B.L. (2013) Amphipol trapping of a functional CYP system. Biotechn. Applied Biochem.
60:119–127.
Le Bon, C., Della Pia, E.A., Giusti, F., Lloret, N., Zoonens, M., Martinez, K.L., Popot, J.-L. (2014a) Synthesis of an
oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins. Nucleic Acids Res. 42:
e83.
Le Bon, C., Popot, J.-L., Giusti, F. (2014b) Labeling and functionalizing amphipols for biological applications.
J. Membr. Biol. 247:797–814.
Le Roy, A., Breyton, C., Ebel, C. (2014) Analytical ultracentrifugation and size-exclusion chromatography coupled with
light scattering for the characterization of membrane proteins in solution, in: Mus-Veteau, I., ed., Membrane Proteins
Production for Structural Analysis. Springer, New York, pp. 267–287.
450
9 Solution Studies of Membrane Protein/Amphipol Complexes
M. (2015) Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins. Biomacromolecules 16:3751–3761.
Gohon, Y. (2002) Etude structurale et fonctionnelle de deux protéines membranaires, la bactériorhodopsine et le
récepteur nicotinique de l’acétylcholine, maintenues en solution aqueuse non détergente par des polymères
amphiphiles. Thèse de Doctorat, Université Paris-VI, Paris, 467 p.
Gohon, Y., Pavlov, G., Timmins, P., Tribet, C., Popot, J.-L., Ebel, C. (2004) Partial specific volume and solvent
interactions of amphipol A8-35. Anal. Biochem. 334:318–334.
Gohon, Y., Giusti, F., Prata, C., Charvolin, D., Timmins, P., Ebel, C., Tribet, C., Popot, J.-L. (2006) Well-defined
nanoparticles formed by hydrophobic assembly of a short and polydisperse random terpolymer, amphipol A8-35.
Langmuir 22:1281–1290.
Gohon, Y., Dahmane, T., Ruigrok, R., Schuck, P., Charvolin, D., Rappaport, F., Timmins, P., Engelman, D.M., Tribet,
C., Popot, J.-L., Ebel, C. (2008) Bacteriorhodopsin/amphipol complexes: structural and functional properties.
Biophys. J. 94:3523–3537.
Gohon, Y., Vindigni, J.-D., Pallier, A., Wien, F., Celia, H., Giuliani, A., Tribet, C., Chardot, T., Briozzo, P. (2011) High
water solubility and fold in amphipols of proteins with large hydrophobic regions: Oleosins and caleosin from seed
lipid bodies. Biochim. Biophys. Acta 1808:706–716.
Guinier, A., Fournet, G. (1955) Small-angle scattering of X-rays. Wiley, New York, 268 p.
Gulati, S., Jamshad, M., Knowles, T.J., Morrison, K.A., Downing, R., Cant, N., Collins, R., Koenderink, J.B., Ford, R.
C., Overduin, M., Kerr, I.D., Dafforn, T.R., Rothnie, A.J. (2014) Detergent-free purification of ABC (ATP-bindingcassette) transporters. Biochem. J. 461:269–278.
Han, S.G., Na, J.H., Lee, W.K., Park, D., Oh, J., Yoon, S.H., Lee, C.K., Sung, M.H., Shin, Y.K., Yu, Y.G. (2014) An
amphipathic polypeptide derived from poly-γ-glutamic acid for the stabilization of membrane proteins. Prot. Sci.
23:1800–1807.
Han, S.G., Baek, S.I., Son, T.J., Lee, H., Kim, N.H., Yu, Y.G. (2017) Preparation of functional human lysophosphatidic
acid receptor 2 using a P9* expression system and an amphipathic polymer and investigation of its in vitro binding
preference to G α proteins. Biochem. Biophys. Res. Commun. 487:103–108.
Harlan, J.E., Picot, D., Loll, P.J., Garavito, R.M. (1995) Calibration of size-exclusion chromatography: use of a double
Gaussian distribution to describe pore sizes. Anal. Biochem. 224:557–563.
Hiruma-Shimizu, K., Shimizu, H., Thompson, G.S., Kalverda, A.P., Patching, S.G. (2015) Deuterated detergents for
structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Mol. Membr.
Biol. 32:139–155.
Huynh, K.W., Cohen, M.R., Moiseenkova-Bell, V.Y. (2014) Application of amphipols for structure-functional analysis
of TRP channels. J. Membr. Biol. 247:843–851.
Jacrot, B. (1976) The study of biological structures by neutron scattering from solution. Rep. Prog. Phys. 39:911–953.
Jamshad, M., Charlton, J., Lin, Y.-P., Routledge, S.J., Bawa, Z., Knowles, T.J., Overduin, M., Dekker, N., Dafforn, T.R.,
Bill, R.M., Poyner, D.R., Wheatley, M. (2015) G protein-coupled receptor solubilization and purification for
biophysical analysis and functional studies, in the total absence of detergent. Biosc. Rep. 35:e00188.
Jin, P., Bulkley, D., Guo, Y., Zhang, W., Guo, Z., Huynh, W., Wu, S., Meltzer, S., Cheng, T., Jan, L.Y., Jan, Y.-N.,
Cheng, Y. (2017) Electron cryo-microscopy structure of the mechanotransduction channel NOMPC. Nature
547:118–122.
Klammt, C., Perrin, M.-H., Maslennikov, I., Renault, L., Krupa, M., Kwiatkowski, W., Stahlberg, H., Vale, W., Choe,
S. (2011) Polymer-based cell-free expression of ligand-binding family B G protein-coupled receptors without
detergents. Prot. Sci. 20:1030–1041.
Knowles, T.J., Finka, R., Smith, C., Lin, Y.-P., Dafforn, T., Overduin, M. (2009) Membrane proteins solubilized intact in
lipid containing nanoparticles bounded by styrene maleic acid copolymer. J. Am. Chem. Soc. 131:7484–7485.
Kraft, T.E., Hresko, R.C., Hruz, P.W. (2015) Expression, purification, and functional characterization of the insulinresponsive facilitative glucose transporter GLUT4. Protein Sci. 24:2008–2019.
Laue, T.M., Stafford, W.E., III (1999) Modern application of analytical ultracentrifugation. Annu. Rev. Biophys. Biomol.
Struct. 28:75–100.
Laursen, T., Naur, P., Møller, B.L. (2013) Amphipol trapping of a functional CYP system. Biotechn. Applied Biochem.
60:119–127.
Le Bon, C., Della Pia, E.A., Giusti, F., Lloret, N., Zoonens, M., Martinez, K.L., Popot, J.-L. (2014a) Synthesis of an
oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins. Nucleic Acids Res. 42:
e83.
Le Bon, C., Popot, J.-L., Giusti, F. (2014b) Labeling and functionalizing amphipols for biological applications.
J. Membr. Biol. 247:797–814.
Le Roy, A., Breyton, C., Ebel, C. (2014) Analytical ultracentrifugation and size-exclusion chromatography coupled with
light scattering for the characterization of membrane proteins in solution, in: Mus-Veteau, I., ed., Membrane Proteins
Production for Structural Analysis. Springer, New York, pp. 267–287.
450
9 Solution Studies of Membrane Protein/Amphipol Complexes