Table 8.2
Fluorescently tagged amphipols and the applications they have been put to. Abbreviations are defined in the legend to Table 8.1.
Label
Carrier
APol
Short
name
Synthesis
and
properties
λ
exc
(nm)
λ
em
(nm)
Demonstrated applications
Studies
Naphthalene A8-75
–
Vial et al.
(2005)
290
310–370 Studying A8-75 interactions with lipid vesicles. Vial et al. (2005, 2007, 2009)
NBD
A8-35
FAPol
NBD
Zoonens
et al. (2007)
470
530
FRET studies of the exchange of FAPol
NBD for
unlabeled A8-35 or detergent at the surface of a
MP. Integration to the plasma membrane of
COS-7 cells and internalization of a
transmembrane peptide and its APol carrier.
Determination of the CAC of A8-35 using
FRET between FAPol
NBD and FAPol
rhod .
Demonstrating the presence of A8-35 in 3D
crystals of cytochrome bc
1 /FAPol
NBD /
detergent complexes. Tracer in immobilization
studies of OligAPol-trapped MPs.
Zoonens et al. (2007), Tribet et al. (2009),
Popot et al. (2011), Giusti et al. (2012),
Charvolin et al. (2014), and Le Bon et al.
(2014a)
Fluorescein
A8-35
FAPol
fluo
Opačić et al.
(2014)
495
540
Quenching of the intrinsic
fluorescence of a
MP.
Opačić et al. (2014)
Rhodamine
A8-35
FAPol
rhod
Giusti et al.
(2012)
555
575
Study of the CAC of A8-35 using FRET
between FAPol
NBD and FAPol
rhod . Study of
in vivo distribution and elimination of A8-35.
Tracer in immobilization studies of MPs
trapped with tagged APols.
Giusti et al. (2012) and Fernandez et al. (2014)
Atto 647
A8-35
FAPol
A647
Unpublished
results
651
667
Study of in vivo distribution and elimination of
A8-35.
Unpublished results
Alexa Fluor
647
A8-35
FAPol
AF647
Fernandez
et al. (2014)
651
668
Study of in vivo distribution and elimination of
A8-35. Tracer in immobilization studies of
MPs trapped with tagged APols.
Della Pia et al. (2014a, b), Fernandez et al.
(2014), and Le Bon et al. (2014a)
398
8 Optical Spectroscopy of Membrane Protein/Amphipol Complexes
Fluorescently tagged amphipols and the applications they have been put to. Abbreviations are defined in the legend to Table 8.1.
Label
Carrier
APol
Short
name
Synthesis
and
properties
λ
exc
(nm)
λ
em
(nm)
Demonstrated applications
Studies
Naphthalene A8-75
–
Vial et al.
(2005)
290
310–370 Studying A8-75 interactions with lipid vesicles. Vial et al. (2005, 2007, 2009)
NBD
A8-35
FAPol
NBD
Zoonens
et al. (2007)
470
530
FRET studies of the exchange of FAPol
NBD for
unlabeled A8-35 or detergent at the surface of a
MP. Integration to the plasma membrane of
COS-7 cells and internalization of a
transmembrane peptide and its APol carrier.
Determination of the CAC of A8-35 using
FRET between FAPol
NBD and FAPol
rhod .
Demonstrating the presence of A8-35 in 3D
crystals of cytochrome bc
1 /FAPol
NBD /
detergent complexes. Tracer in immobilization
studies of OligAPol-trapped MPs.
Zoonens et al. (2007), Tribet et al. (2009),
Popot et al. (2011), Giusti et al. (2012),
Charvolin et al. (2014), and Le Bon et al.
(2014a)
Fluorescein
A8-35
FAPol
fluo
Opačić et al.
(2014)
495
540
Quenching of the intrinsic
fluorescence of a
MP.
Opačić et al. (2014)
Rhodamine
A8-35
FAPol
rhod
Giusti et al.
(2012)
555
575
Study of the CAC of A8-35 using FRET
between FAPol
NBD and FAPol
rhod . Study of
in vivo distribution and elimination of A8-35.
Tracer in immobilization studies of MPs
trapped with tagged APols.
Giusti et al. (2012) and Fernandez et al. (2014)
Atto 647
A8-35
FAPol
A647
Unpublished
results
651
667
Study of in vivo distribution and elimination of
A8-35.
Unpublished results
Alexa Fluor
647
A8-35
FAPol
AF647
Fernandez
et al. (2014)
651
668
Study of in vivo distribution and elimination of
A8-35. Tracer in immobilization studies of
MPs trapped with tagged APols.
Della Pia et al. (2014a, b), Fernandez et al.
(2014), and Le Bon et al. (2014a)
398
8 Optical Spectroscopy of Membrane Protein/Amphipol Complexes