Table 8.1
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.15
Diphtheria toxin T-domain A8-35, NAPol
●
●
CD and
fluorescence studies of
the conformation of diphtheria
toxin T-domain and lipid
vesicle permeabilization as a
function of pH, temperature,
and the environment (detergent,
fluorinated surfactant, APols, or
lipid vesicles)
Kyrychenko et al. (2012)
8.16
OmpT and PagP from
E. coli
A8-35
●
Amphipol-assisted folding and
mass spectrometry of OmpT
and PagP
Leney et al. (2012)
8.17
Immunoglobulin G (IgG;
not a membrane protein)
A8-35 and other
PAA-derived polymers
●
The thermal stability and
solubility of IgG in water were
monitored by differential
scanning calorimetry (DSC),
dynamic light scattering (DLS),
fluorescence correlation
spectroscopy (FCS), and
asymmetrical
flow field-flow
fractionation (AF4) in the
presence of various polymers
Ma et al. (2012, 2014) and
Martin et al. (2014)
8.18
BR
SMA
●
Visible CD study of the
association state of BR in the
purple membrane, in SMALPs,
and in lipid vesicles
Orwick-Rydmark et al. (2012)
8.19
Arginine-vasopressin Type
2 GPCR (V2R)
NAPol
●
Tryptophan intrinsic
fluorescence spectroscopy and
fluorescence quenching and
lanthanide resonance energy
transfer (LRET) studies of
ligand and arrestin binding to
V2R
Rahmeh et al. (2012)
(continued)
8.2 Optical Spectroscopy Studies of Amphipols and Membrane Protein/Amphipol Complexes
385
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.15
Diphtheria toxin T-domain A8-35, NAPol
●
●
CD and
fluorescence studies of
the conformation of diphtheria
toxin T-domain and lipid
vesicle permeabilization as a
function of pH, temperature,
and the environment (detergent,
fluorinated surfactant, APols, or
lipid vesicles)
Kyrychenko et al. (2012)
8.16
OmpT and PagP from
E. coli
A8-35
●
Amphipol-assisted folding and
mass spectrometry of OmpT
and PagP
Leney et al. (2012)
8.17
Immunoglobulin G (IgG;
not a membrane protein)
A8-35 and other
PAA-derived polymers
●
The thermal stability and
solubility of IgG in water were
monitored by differential
scanning calorimetry (DSC),
dynamic light scattering (DLS),
fluorescence correlation
spectroscopy (FCS), and
asymmetrical
flow field-flow
fractionation (AF4) in the
presence of various polymers
Ma et al. (2012, 2014) and
Martin et al. (2014)
8.18
BR
SMA
●
Visible CD study of the
association state of BR in the
purple membrane, in SMALPs,
and in lipid vesicles
Orwick-Rydmark et al. (2012)
8.19
Arginine-vasopressin Type
2 GPCR (V2R)
NAPol
●
Tryptophan intrinsic
fluorescence spectroscopy and
fluorescence quenching and
lanthanide resonance energy
transfer (LRET) studies of
ligand and arrestin binding to
V2R
Rahmeh et al. (2012)
(continued)
8.2 Optical Spectroscopy Studies of Amphipols and Membrane Protein/Amphipol Complexes
385