Table 8.1
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.8
PagP expressed in E. coli
and BR from
Halobacterium salinarum
SMA
●
CD studies of the secondary
structure and thermal
denaturation of PagP and BR in
SMALPs, liposomes, or
detergent
Knowles et al. (2009)
8.9
BR
A8-35
●
Secondary structure
investigations of BR in either
detergent solution or trapped
with A8-35, either native or
refolded in the presence or
absence of lipids
Popot et al. (2011)
8.10
Oleosins and caleosin
(hydrophobic,
non-membrane proteins)
from Arabidopsis thaliana
seed lipid bodies
A8-35 and other
random or blocky
PAA-derived APols
●
Secondary structure studies of
oleosins and caleosin in various
environments
Gohon et al. (2011)
8.11
Major outer membrane
protein (MOMP) from
Chlamydia trachomatis
A8-35
●
CD studies of the
thermostability of MOMP in a
detergent vs. an APol
environment
Tifrea et al. (2011) and
Feinstein et al. (2014)
8.12
Cultures of COS-7 cells
FAPol
NBD
●
Integration to the plasma
membrane and internalization
of a transmembrane peptide and
its APol carrier
Popot et al. (2011)
8.13
Ghrelin receptor (GPCR)
overexpressed in E. coli
NAPols
●
●
FRET measurements of the
binding of ghrelin to the
receptor folded in NAPols from
SDS-solubilized inclusion
bodies
Bazzacco et al. (2012)
8.14
No protein
A8-35, FAPol
NBD ,
FAPol
rhod
●
A study of the critical
aggregation concentration
(CAC) of A8-35 using FRET
between FAPol
NBD and
FAPol
rhod
Giusti et al. (2012)
384
8 Optical Spectroscopy of Membrane Protein/Amphipol Complexes