Fig. 5.28 Stabilization of a G protein-coupled receptor by A8-35 in the presence or absence of lipids. The
BLT1 receptor of leukotriene LTB 4 was either trapped by A8-35 or by an A8-35/asolectin mixture (5:1
w/w) or kept in a fos-choline-16/asolectin solution (2:1 w/w). Left. Stability upon incubation at increasing
temperature. Right. Stability upon extended storage at 4
C. D + L, fos-choline-16/asolectin mixed
micelles; AP, A8-35; AP+L, A8-35 + asolectin (From Popot 2010, adapted with permission from
Dahmane et al. 2009, # 2009 American Chemical Society).
Fig. 5.29 Stability of the insulin-responsive facilitative glucose transporter GLUT4 solubilized in
dodecylmaltoside (DDM) or 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG) or trapped with
A8-35, as determined by size exclusion chromatography (SEC). (A, B) Green fluorescent protein-tagged
GLUT4 was solubilized in either DDM (A) or LMNG (B) and incubated at 4
C for 68 h. Aliquots were
analyzed by fluorescence SEC after 0, 20, 44, and 68 h. C. Stabilizing effect of A8-35 as compared to
LMNG. A8-35-trapped GLUT4 incubated at 40
C for 2 h and analyzed by fluorescence SEC before (dark
blue) and after (light blue) incubation, as compared with LMNG-solubilized GLUT4 after incubation at
40
C for 2 h (red) (From Kraft et al. 2015, # 2015 The Protein Society).
5.5 Biochemical Stability of Amphipol-Trapped Membrane Proteins
293
BLT1 receptor of leukotriene LTB 4 was either trapped by A8-35 or by an A8-35/asolectin mixture (5:1
w/w) or kept in a fos-choline-16/asolectin solution (2:1 w/w). Left. Stability upon incubation at increasing
temperature. Right. Stability upon extended storage at 4
C. D + L, fos-choline-16/asolectin mixed
micelles; AP, A8-35; AP+L, A8-35 + asolectin (From Popot 2010, adapted with permission from
Dahmane et al. 2009, # 2009 American Chemical Society).
Fig. 5.29 Stability of the insulin-responsive facilitative glucose transporter GLUT4 solubilized in
dodecylmaltoside (DDM) or 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG) or trapped with
A8-35, as determined by size exclusion chromatography (SEC). (A, B) Green fluorescent protein-tagged
GLUT4 was solubilized in either DDM (A) or LMNG (B) and incubated at 4
C for 68 h. Aliquots were
analyzed by fluorescence SEC after 0, 20, 44, and 68 h. C. Stabilizing effect of A8-35 as compared to
LMNG. A8-35-trapped GLUT4 incubated at 40
C for 2 h and analyzed by fluorescence SEC before (dark
blue) and after (light blue) incubation, as compared with LMNG-solubilized GLUT4 after incubation at
40
C for 2 h (red) (From Kraft et al. 2015, # 2015 The Protein Society).
5.5 Biochemical Stability of Amphipol-Trapped Membrane Proteins
293