Table 4.3 Main characteristics of A8-35 particles.
Estimated error
Samples
HAPol-1
DAPol-1
Size exclusion chromatography
R S(SEC) (nm)
Æ0.15
3.15
3.15
Static light scattering
M(kgÁmol
À1 )
Æ5
pH 6.8
98
pH 7.5
112
pH 8.0
93
pH 9.2
97
A 2 (mLÁg
À1
)
a
Æ0.01
pH 6.8
15
pH 7.5
13
pH 8.0
30
pH 9.2
50
Dynamic light scattering
R S(DLS) (nm)
pH 6.8
3.6
pH 9.2
3.6
Small-angle neutron scattering
R g
(nm)
b
Æ0.2
2.4
2.4
R s(SANS) (nm)
c
Æ0.25
3.1
3.1
(∂ρ N /∂C) μ (cmÁg
À1
)
d
Æ0.1 Â 10
10
1.1 Â 10
10
4.4 Â 10
10
M SANS (kgÁmol
À1
)
e
Æ1.5
46
34
Equilibrium ultracentrifugation
M eq (kgÁmol
À1
)
f
Æ7
2 8
4 2
Sedimentation velocity AUC
S 20,w (S) (mass%)
g
Æ0.1
5
C
1.5 (92%)
2.2 (92%)
20
C
1.6 (80%)
2.2 (70%)
ϕ
0 (mLÁg
À1
)
h
Æ0.004
0.866
0.820
S 20,w /(1 À ϕ
0 ρ) (S)
Æ0.1
12
12
M SV (kgÁmol
À1
)
i
Æ5
4 2
4 4
Combined data
hMi (kgÁmol
À1
)
j
Æ5
3 9
4 0
v 2 (mLÁg
À1 )
k
Æ0.003
0.809
0.765
R min (nm)
l
Æ0.1
2.3
2.3
R s(SEC) /R min
Æ0.1
1.37
1.37
δ (g H 2 O/g APol)
m
Æ0.45
1.22
1.21
The table is from Gohon et al. (2006)
The table summarizes which information has been obtained by which of the following techniques: size
exclusion chromatography (SEC), static light scattering (SLS), dynamic light scattering (DLS), smallangle neutron scattering (SANS), and equilibrium and sedimentation velocity analytical ultracentrifugation
(Eq-AUC and SV-AUC, respectively) . The last section (“Combined Data”) proposes a consensus picture
of A8-35 particles, taking into account the relative accuracy and reliability of each technique. Two A8-35
preparations were used to gather the data, an unlabeled one (“HAPol-1”) and a partially deuterated one
(“DAPol-1”)
a Second virial coefficient. In order to avoid aggregation, SLS and DLS measurements carried out at pH 6.8
were performed immediately (minutes) after samples were diluted into the buffer
b
Radius of gyration at infinite contrast, R g
, deduced from Stuhrmann plots
c Stokes radius, calculated from R g
assuming the particles to be spherical
180
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols
Estimated error
Samples
HAPol-1
DAPol-1
Size exclusion chromatography
R S(SEC) (nm)
Æ0.15
3.15
3.15
Static light scattering
M(kgÁmol
À1 )
Æ5
pH 6.8
98
pH 7.5
112
pH 8.0
93
pH 9.2
97
A 2 (mLÁg
À1
)
a
Æ0.01
pH 6.8
15
pH 7.5
13
pH 8.0
30
pH 9.2
50
Dynamic light scattering
R S(DLS) (nm)
pH 6.8
3.6
pH 9.2
3.6
Small-angle neutron scattering
R g
(nm)
b
Æ0.2
2.4
2.4
R s(SANS) (nm)
c
Æ0.25
3.1
3.1
(∂ρ N /∂C) μ (cmÁg
À1
)
d
Æ0.1 Â 10
10
1.1 Â 10
10
4.4 Â 10
10
M SANS (kgÁmol
À1
)
e
Æ1.5
46
34
Equilibrium ultracentrifugation
M eq (kgÁmol
À1
)
f
Æ7
2 8
4 2
Sedimentation velocity AUC
S 20,w (S) (mass%)
g
Æ0.1
5
C
1.5 (92%)
2.2 (92%)
20
C
1.6 (80%)
2.2 (70%)
ϕ
0 (mLÁg
À1
)
h
Æ0.004
0.866
0.820
S 20,w /(1 À ϕ
0 ρ) (S)
Æ0.1
12
12
M SV (kgÁmol
À1
)
i
Æ5
4 2
4 4
Combined data
hMi (kgÁmol
À1
)
j
Æ5
3 9
4 0
v 2 (mLÁg
À1 )
k
Æ0.003
0.809
0.765
R min (nm)
l
Æ0.1
2.3
2.3
R s(SEC) /R min
Æ0.1
1.37
1.37
δ (g H 2 O/g APol)
m
Æ0.45
1.22
1.21
The table is from Gohon et al. (2006)
The table summarizes which information has been obtained by which of the following techniques: size
exclusion chromatography (SEC), static light scattering (SLS), dynamic light scattering (DLS), smallangle neutron scattering (SANS), and equilibrium and sedimentation velocity analytical ultracentrifugation
(Eq-AUC and SV-AUC, respectively) . The last section (“Combined Data”) proposes a consensus picture
of A8-35 particles, taking into account the relative accuracy and reliability of each technique. Two A8-35
preparations were used to gather the data, an unlabeled one (“HAPol-1”) and a partially deuterated one
(“DAPol-1”)
a Second virial coefficient. In order to avoid aggregation, SLS and DLS measurements carried out at pH 6.8
were performed immediately (minutes) after samples were diluted into the buffer
b
Radius of gyration at infinite contrast, R g
, deduced from Stuhrmann plots
c Stokes radius, calculated from R g
assuming the particles to be spherical
180
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols