GDP-glucose: ceramide transferase into different thicknesses of lipid membranes
and the enzyme activities of those cases. Different phosphatidylcholines with saturated fatty acyl chains from 15 carbons to 18 carbons are used for different thicknesses of lipid bilayer membranes. And the maximum activity obtained when the
enzyme is reconstructed in dimyristoylphosphatidylcholine (12 carbons fatty acid),
which means the enzyme takes optimum configuration in interaction with
dimyristoylphosphatidylcholine.
References
1. L. Michaelis, M. Menten, Biochem. Z. 49, 333 (1913)
2. E. Briggs, J.B.S. Haldane, Biochem. J. 19 (1925), 338(1925)
3. Jacob, J. Monod, J. Mol. Biol. 3, 318 (1961)
4. J. Monod, J. Wyman, J.P. Changeux, Mol. Biol. 12, 88 (1965)
5. D.E. Koshland Jr., G. Nemethy, D. Filmer, Biochemistry 5, 365 (1966)
6. S.J. Singer, G.L. Nicolson, Science 175, 720 (1972)
7. P.C. Jost, O.H. Griffith, R.A. Capaldi, G. Vanderkooi, Proc. Natl. Acad. Sci. USA 70,
480 (1973)
8. T. Tsukihara, T.H. Aoyama, E. Yamaashita, T. Tomizaki, H. Yamaguchi, H.K. Shinzawa-Itoh,
R. Nakahima, R. Yaono, S. Yoshikawa, Science 269, 1069 (1995)
9. E.M. Landau, J.P. Rosenbusch, Proc. Natl. Acad. Sci. USA 93, 14532 (1996)
10. B. de Kruijff, Nature 386, 129 (1977)
11. R.N. McElhaney, Biochim. Biophys. Acta 864, 361 (1986)
Length of acyl chains used for reconstruction of enzyme
C6:0 C8:0 C10:0 C11:0 C12:0 C13:0 C14:0 C15:0 C16:0 C18:0
Relative enzyme activity[% of PC12:0]
0
25
50
75
100
Fig. 5.3 Enzyme activities of UDP-glucose: ceramide glucose transferase UDP-glucose: ceramide
glucose transferase was reconstructed in phosphatidylcholine with different lengths of fatty acyl
chains. And the enzyme activity was measured. Optimum length of fatty acyl chain existed for the
activity
References
79
and the enzyme activities of those cases. Different phosphatidylcholines with saturated fatty acyl chains from 15 carbons to 18 carbons are used for different thicknesses of lipid bilayer membranes. And the maximum activity obtained when the
enzyme is reconstructed in dimyristoylphosphatidylcholine (12 carbons fatty acid),
which means the enzyme takes optimum configuration in interaction with
dimyristoylphosphatidylcholine.
References
1. L. Michaelis, M. Menten, Biochem. Z. 49, 333 (1913)
2. E. Briggs, J.B.S. Haldane, Biochem. J. 19 (1925), 338(1925)
3. Jacob, J. Monod, J. Mol. Biol. 3, 318 (1961)
4. J. Monod, J. Wyman, J.P. Changeux, Mol. Biol. 12, 88 (1965)
5. D.E. Koshland Jr., G. Nemethy, D. Filmer, Biochemistry 5, 365 (1966)
6. S.J. Singer, G.L. Nicolson, Science 175, 720 (1972)
7. P.C. Jost, O.H. Griffith, R.A. Capaldi, G. Vanderkooi, Proc. Natl. Acad. Sci. USA 70,
480 (1973)
8. T. Tsukihara, T.H. Aoyama, E. Yamaashita, T. Tomizaki, H. Yamaguchi, H.K. Shinzawa-Itoh,
R. Nakahima, R. Yaono, S. Yoshikawa, Science 269, 1069 (1995)
9. E.M. Landau, J.P. Rosenbusch, Proc. Natl. Acad. Sci. USA 93, 14532 (1996)
10. B. de Kruijff, Nature 386, 129 (1977)
11. R.N. McElhaney, Biochim. Biophys. Acta 864, 361 (1986)
Length of acyl chains used for reconstruction of enzyme
C6:0 C8:0 C10:0 C11:0 C12:0 C13:0 C14:0 C15:0 C16:0 C18:0
Relative enzyme activity[% of PC12:0]
0
25
50
75
100
Fig. 5.3 Enzyme activities of UDP-glucose: ceramide glucose transferase UDP-glucose: ceramide
glucose transferase was reconstructed in phosphatidylcholine with different lengths of fatty acyl
chains. And the enzyme activity was measured. Optimum length of fatty acyl chain existed for the
activity
References
79