Table 5 Physicochemical parameters for extraction of chitin by chemical and biological processes (adapted from [30])
Parameters
Chemical treatment
Biological treatment
Concentration of acid
2 N HCl
2 N HCl
2 N HCl
69.5 g/L lactic acid
a
69.5 g/L lactic acid
a
69.5 g/L lactic acid
a
Temperature
RT
RT
RT
40
C
4 0
C
4 0
C
Shell to acid ratio
1:15
1:25
1:35
1:15
1:25
1:35
Concentration of deproteinizer
1 N NaOH
1 N NaOH
1 N NaOH
5 g wet weight
b
5 g wet weight
b
5 g wet weight
b
Product appearance
White
Super-white
Super-white
Slightly brownish
White
White
Yield (g)
5.62
5.47
5.41
4.52
4.17
4.03
Yield (%)
28.1
27.35
27.05
22.6
20.85
20.15
Solubility in water
Insoluble
Insoluble
Insoluble
Partially soluble
Partially soluble
Partially soluble
pH
7.2
7.2
7.2
6.9
6.9
6.9
RT room temperature
a
Lactic acid was produced by
Lactobacillus plantarum
b
Deproteinization was done using
Aspergillus Niger, which produces proteolytic enzymes
96
J. Dutta