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{
.
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effect of side chain length. Macromolecules 39:8440–8450. doi:10.1021/ma0613178
87. Sahl M, Muth S, Branscheid R et al (2012) Helix–coil transition in cylindrical brush polymers
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88. Zhang B, Fischer K, Schmidt M (2005) Cylindrical polypeptide brushes. Macromol Chem
Phys 206:157–162. doi:10.1002/macp.200400266
89. Sto ¨rkle D, Duschner S, Heimann N et al (2007) Complex formation of DNA with oppositely
charged polyelectrolytes of different chain topology: cylindrical brushes and dendrimers.
Macromolecules 40:7998–8006. doi:10.1021/ma0711689
90. Boeckle S, Von Gersdorff K, Van der Piepen S et al (2004) Purification of polyethylenimine
polyplexes highlights the role of free polycations in gene transfer. J Gene Med 6:1102–1111.
doi:10.1002/jgm.598
91. Yue Y, Jin F, Deng R et al (2011) Revisit complexation between DNA and polyethylenimine
– effect of uncomplexed chains free in the solution mixture on gene transfection. J Control
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92. Bertschinger M, Backliwal G, Schertenleib A et al (2006) Disassembly of polyethylenimineDNA particles in vitro: implications for polyethylenimine-mediated DNA delivery. J Control
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93. Moret I, Esteban Peris J, Guillem VM et al (2001) Stability of PEI-DNA and DOTAP-DNA
complexes: effect of alkaline pH, heparin and serum. J Control Release 76:169–181
94. Ku ¨hn F (2010) Polykation-DNA-komplexe: eigenschaften und anwendungen in der
gentransfektion. Dissertation, Johannes Gutenberg-Universita ¨t Mainz
95. Li P, Liu D, Sun X et al (2011) A novel cationic liposome formulation for efficient gene
delivery via a pulmonary route. Nanotechnology 22:245104. doi:10.1088/0957-4484/22/24/
245104
96. Buzder T, Yin X, Wang X et al (2009) Uptake of foreign nucleic acids in kidney tubular
epithelial cells deficient in proapoptotic endonucleases. DNA Cell Biol 28:435–442.
doi:10.1089/dna.2008.0850
97. Gadi J, Ruthala K, Kong K-A et al (2009) The third helix of the Hoxc8 homeodomain peptide
enhances the efficiency of gene transfer in combination with lipofectamine. Mol Biotechnol
42:41–48. doi:10.1007/s12033-008-9119-7
98. Howell DP-G, Krieser RJ, Eastman A, Barry MA (2003) Deoxyribonuclease II is a lysosomal
barrier to transfection. Mol Ther 8:957–963
99. Fischer D, Li Y, Ahlemeyer B et al (2003) In vitro cytotoxicity testing of polycations:
influence of polymer structure on cell viability and hemolysis. Biomaterials 24:1121–1131
100. Mahringer A, Ott M, Reimold I et al (2011) The ABC of the blood–brain barrier – regulation
of drug efflux pumps. Curr Pharm Des 17:2762–2770
101. Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T (2002) Comparison of the efficiency
and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol
Pharm Bull 25:891–897
Polymer Complexes in Biological Applications
235
behavior of cylindrical brushes as revealed by GPC/MALLS, light scattering, and SFM
{
.
Macromolecules 32:2629–2637. doi:10.1021/ma9816463
86. Zhang B, Gro ¨hn F, Pedersen JS et al (2006) Conformation of cylindrical brushes in solution:
effect of side chain length. Macromolecules 39:8440–8450. doi:10.1021/ma0613178
87. Sahl M, Muth S, Branscheid R et al (2012) Helix–coil transition in cylindrical brush polymers
with poly-L-lysine side chains. Macromolecules 45:5167–5175. doi:10.1021/ma300377v
88. Zhang B, Fischer K, Schmidt M (2005) Cylindrical polypeptide brushes. Macromol Chem
Phys 206:157–162. doi:10.1002/macp.200400266
89. Sto ¨rkle D, Duschner S, Heimann N et al (2007) Complex formation of DNA with oppositely
charged polyelectrolytes of different chain topology: cylindrical brushes and dendrimers.
Macromolecules 40:7998–8006. doi:10.1021/ma0711689
90. Boeckle S, Von Gersdorff K, Van der Piepen S et al (2004) Purification of polyethylenimine
polyplexes highlights the role of free polycations in gene transfer. J Gene Med 6:1102–1111.
doi:10.1002/jgm.598
91. Yue Y, Jin F, Deng R et al (2011) Revisit complexation between DNA and polyethylenimine
– effect of uncomplexed chains free in the solution mixture on gene transfection. J Control
Release 155:67–76. doi:10.1016/j.jconrel.2010.10.028
92. Bertschinger M, Backliwal G, Schertenleib A et al (2006) Disassembly of polyethylenimineDNA particles in vitro: implications for polyethylenimine-mediated DNA delivery. J Control
Release 116:96–104. doi:10.1016/j.jconrel.2006.09.006
93. Moret I, Esteban Peris J, Guillem VM et al (2001) Stability of PEI-DNA and DOTAP-DNA
complexes: effect of alkaline pH, heparin and serum. J Control Release 76:169–181
94. Ku ¨hn F (2010) Polykation-DNA-komplexe: eigenschaften und anwendungen in der
gentransfektion. Dissertation, Johannes Gutenberg-Universita ¨t Mainz
95. Li P, Liu D, Sun X et al (2011) A novel cationic liposome formulation for efficient gene
delivery via a pulmonary route. Nanotechnology 22:245104. doi:10.1088/0957-4484/22/24/
245104
96. Buzder T, Yin X, Wang X et al (2009) Uptake of foreign nucleic acids in kidney tubular
epithelial cells deficient in proapoptotic endonucleases. DNA Cell Biol 28:435–442.
doi:10.1089/dna.2008.0850
97. Gadi J, Ruthala K, Kong K-A et al (2009) The third helix of the Hoxc8 homeodomain peptide
enhances the efficiency of gene transfer in combination with lipofectamine. Mol Biotechnol
42:41–48. doi:10.1007/s12033-008-9119-7
98. Howell DP-G, Krieser RJ, Eastman A, Barry MA (2003) Deoxyribonuclease II is a lysosomal
barrier to transfection. Mol Ther 8:957–963
99. Fischer D, Li Y, Ahlemeyer B et al (2003) In vitro cytotoxicity testing of polycations:
influence of polymer structure on cell viability and hemolysis. Biomaterials 24:1121–1131
100. Mahringer A, Ott M, Reimold I et al (2011) The ABC of the blood–brain barrier – regulation
of drug efflux pumps. Curr Pharm Des 17:2762–2770
101. Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T (2002) Comparison of the efficiency
and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol
Pharm Bull 25:891–897
Polymer Complexes in Biological Applications
235