199. Yang J, Zhang P, Tang L, Sun P, Liu W, Sun P, Zuo A, Liang D (2010) Temperature-tuned
DNA condensation and gene transfection by PEI-g-(PMEO 2 MA-b-PHEMA) copolymerbased nonviral vectors. Biomaterials 31:144–155
200. Tv E, Zwicker S, Pidhatika B, Konradi R, Textor M, Hall H, Lu ¨hmann T (2011) Formation
and characterization of DNA-polymer-condensates based on poly(2-methyl-2-oxazoline)
grafted poly(L-lysine) for non-viral delivery of therapeutic DNA. Biomaterials
32:5291–5303
201. Bikram M, Ahn C-H, Chae SY, Lee M, Yockman JW, Kim SW (2004) Biodegradable poly
(ethylene glycol)-co-poly(L-lysine)-g-histidine multiblock copolymers for nonviral gene
delivery. Macromolecules 37:1903–1916
202. Putnam D, Zelikin AN, Izumrudov VA, Langer R (2003) Polyhistidine-PEG: DNA
nanocomposites for gene delivery. Biomaterials 24:4425–4433
203. Kanayama N, Fukushima S, Nishiyama N, Itaka K, Jang W-D, Miyata K, Yamasaki Y,
Chung U-I, Kataoka K (2006) A PEG-based biocompatible block catiomer with high
buffering capacity for the construction of polyplex micelles showing efficient gene transfer
toward primary cells. ChemMedChem 1:439–444
204. Miyata K, Fukushima S, Nishiyama N, Yamasaki Y, Kataoka K (2007) PEG-based block
catiomers possessing DNA anchoring and endosomal escaping functions to form polyplex
micelles with improved stability and high transfection efficacy. J Control Release
122:252–260
205. Vuillaume PY, Brunelle M, Calsteren M-RV, Laurent-Lewandowski S, Begin A,
Lewandowski R, Talbot BG, ElAzhary Y (2005) Synthesis and characterization of new
permanently charged poly(amidoammonium) salts and evaluation of their DNA complexes
for gene transport. Biomacromolecules 6:1769–1781
206. Thomas M, Klibanov AM (2002) Enhancing polyethylenimine’s delivery of plasmid DNA
into mammalian cells. Proc Natl Acad Sci USA 99:14640–14645
207. Kabanov AV, Astafyeva IV, Chikindas ML, Rosenblat CF, Kiselev VI, Severin ES, Kabanov
VA (1991) DNA interpolyelectrolyte complexes as a tool for efficient cell transformation.
Biopolymers 31:1437–1443
208. Doody AM, Korley JN, Dang KP, Zawaneh PN, Putnam D (2006) Characterizing the
structure/function parameter space of hydrocarbon-conjugated branched polyethylenimine
for DNA delivery in vitro. J Control Release 116:227–237
209. Liu Z, Zhang Z, Zhou C, Jiao Y (2010) Hydrophobic modifications of cationic polymers for
gene delivery. Prog Polym Sci 35:1144–1162
210. Juan AS, Letourneur D, Izumrudov VA (2007) Quaternized poly(4-vinylpyridine)s as model
gene delivery polycations: structure-function study by modification of side chain
hydrophobicity and degree of alkylation. Bioconjug Chem 18:922–928
211. Vuillaume PY, Brunelle M, Bazuin CG, Talbot BG, Begin A, Calsteren M-RV, LaurentLewandowski S (2009) Tail-end amphiphilic dimethylaminopyridinium-containing
polymethacrylates for gene delivery. New J Chem 33:1941–1950
212. Asayama S, Hakamatani T, Kawakami H (2010) Synthesis and characterization of alkylated
poly(1-vinylimidazole) to control the stability of its DNA polyion complexes for gene
delivery. Bioconjug Chem 21:646–652
213. Filippov SK, Konak C, Kopeckov P, Starovoytova L, Spirkova M, Stepanek P (2010) Effect
of hydrophobic interactions on properties and stability of DNA-Polyelectrolyte complexes.
Langmuir 26:4999–5006
214. Kabanov AV, Kabanov VA (1995) DNA complexes with polycations for the delivery of
genetic material into cells. Bioconjug Chem 6:7–20
215. Oupicky D, Konak C, Ulbrich K, Wolfert MA, Seymour LW (2000) DNA delivery systems
based on complexes of DNA with synthetic polycations and their copolymers. J Control
Release 65:149–171
216. Sun X, Liu C, Liu D, Li P, Zhang N (2012) Novel biomimetic vectors with endosomal-escape
agent enhancing gene transfection efficiency. Int J Pharm 425:62–72
Polyelectrolyte Complexes of DNA and Polycations as Gene Delivery Vectors
193
DNA condensation and gene transfection by PEI-g-(PMEO 2 MA-b-PHEMA) copolymerbased nonviral vectors. Biomaterials 31:144–155
200. Tv E, Zwicker S, Pidhatika B, Konradi R, Textor M, Hall H, Lu ¨hmann T (2011) Formation
and characterization of DNA-polymer-condensates based on poly(2-methyl-2-oxazoline)
grafted poly(L-lysine) for non-viral delivery of therapeutic DNA. Biomaterials
32:5291–5303
201. Bikram M, Ahn C-H, Chae SY, Lee M, Yockman JW, Kim SW (2004) Biodegradable poly
(ethylene glycol)-co-poly(L-lysine)-g-histidine multiblock copolymers for nonviral gene
delivery. Macromolecules 37:1903–1916
202. Putnam D, Zelikin AN, Izumrudov VA, Langer R (2003) Polyhistidine-PEG: DNA
nanocomposites for gene delivery. Biomaterials 24:4425–4433
203. Kanayama N, Fukushima S, Nishiyama N, Itaka K, Jang W-D, Miyata K, Yamasaki Y,
Chung U-I, Kataoka K (2006) A PEG-based biocompatible block catiomer with high
buffering capacity for the construction of polyplex micelles showing efficient gene transfer
toward primary cells. ChemMedChem 1:439–444
204. Miyata K, Fukushima S, Nishiyama N, Yamasaki Y, Kataoka K (2007) PEG-based block
catiomers possessing DNA anchoring and endosomal escaping functions to form polyplex
micelles with improved stability and high transfection efficacy. J Control Release
122:252–260
205. Vuillaume PY, Brunelle M, Calsteren M-RV, Laurent-Lewandowski S, Begin A,
Lewandowski R, Talbot BG, ElAzhary Y (2005) Synthesis and characterization of new
permanently charged poly(amidoammonium) salts and evaluation of their DNA complexes
for gene transport. Biomacromolecules 6:1769–1781
206. Thomas M, Klibanov AM (2002) Enhancing polyethylenimine’s delivery of plasmid DNA
into mammalian cells. Proc Natl Acad Sci USA 99:14640–14645
207. Kabanov AV, Astafyeva IV, Chikindas ML, Rosenblat CF, Kiselev VI, Severin ES, Kabanov
VA (1991) DNA interpolyelectrolyte complexes as a tool for efficient cell transformation.
Biopolymers 31:1437–1443
208. Doody AM, Korley JN, Dang KP, Zawaneh PN, Putnam D (2006) Characterizing the
structure/function parameter space of hydrocarbon-conjugated branched polyethylenimine
for DNA delivery in vitro. J Control Release 116:227–237
209. Liu Z, Zhang Z, Zhou C, Jiao Y (2010) Hydrophobic modifications of cationic polymers for
gene delivery. Prog Polym Sci 35:1144–1162
210. Juan AS, Letourneur D, Izumrudov VA (2007) Quaternized poly(4-vinylpyridine)s as model
gene delivery polycations: structure-function study by modification of side chain
hydrophobicity and degree of alkylation. Bioconjug Chem 18:922–928
211. Vuillaume PY, Brunelle M, Bazuin CG, Talbot BG, Begin A, Calsteren M-RV, LaurentLewandowski S (2009) Tail-end amphiphilic dimethylaminopyridinium-containing
polymethacrylates for gene delivery. New J Chem 33:1941–1950
212. Asayama S, Hakamatani T, Kawakami H (2010) Synthesis and characterization of alkylated
poly(1-vinylimidazole) to control the stability of its DNA polyion complexes for gene
delivery. Bioconjug Chem 21:646–652
213. Filippov SK, Konak C, Kopeckov P, Starovoytova L, Spirkova M, Stepanek P (2010) Effect
of hydrophobic interactions on properties and stability of DNA-Polyelectrolyte complexes.
Langmuir 26:4999–5006
214. Kabanov AV, Kabanov VA (1995) DNA complexes with polycations for the delivery of
genetic material into cells. Bioconjug Chem 6:7–20
215. Oupicky D, Konak C, Ulbrich K, Wolfert MA, Seymour LW (2000) DNA delivery systems
based on complexes of DNA with synthetic polycations and their copolymers. J Control
Release 65:149–171
216. Sun X, Liu C, Liu D, Li P, Zhang N (2012) Novel biomimetic vectors with endosomal-escape
agent enhancing gene transfection efficiency. Int J Pharm 425:62–72
Polyelectrolyte Complexes of DNA and Polycations as Gene Delivery Vectors
193