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121. Chen YM, Yu CJ, Cheng TL et al (2008) Colorimetric detection of lysozyme based on
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122. Bayraktar H, Srivastava S, You C et al (2008) Controlled nanoparticle assembly through
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129. Howell N, Yeboah N, Lewis D (1995) Studies on the electrostatic interactions of lysozyme
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130. Biesheuvel PM, Lindhoud S, de Vries R et al (2006) Phase behavior of mixtures of oppositely
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8(16):4471–4478
133. Tiwari A, Bindal S, Bohidar HB (2009) Kinetics of protein-protein complex coacervation and
biphasic release of salbutamol sulfate from coacervate matrix. Biomacromolecules
10:184–189
134. Nigen M, Croguennec T, Madec MN et al (2007) Apo alpha-lactalbumin and lysozyme are
colocalized in their subsequently formed spherical supramolecular assembly. FEBS J
274:6085–6093
135. Nigen M, Le Tilly V, Croguennec T et al (2009) Molecular interaction between apo or holo αlactalbumin and lysozyme: formation of heterodimers as assessed by fluorescence
measurements. Biochim Biophys Acta 1794:709–715
136. Salvatore D, Duraffourg N, Favier A et al (2011) Investigation at residue level of the early
steps during the assembly of two proteins into supramolecular objects. Biomacromolecules
12(6):2200–2210
137. Shinoda W, DeVane R, Klein ML (2012) Computer simulation studies of self-assembling
macromolecules. Curr Opin Struct Biol 22:1–12
138. Salvatore D, Croguennec T, Bouhallab S et al (2011) Kinetics and structure during self-assembly
of oppositely charged proteins in aqueous solution. Biomacromolecules 12(5):1920–6192
139. Persson BA, Lund M (2009) Association and electrostatic steering of α-lactalbumin–
lysozyme heterodimers. Phys Chem Chem Phys 11:8879–8885
140. Xu Y, Mazzawi M, Chen K et al (2011) Protein purification by polyelectrolyte coacervation:
influence of protein charge anisotropy on selectivity. Biomacromolecules 12:1512–1522
100
S. Bouhallab and T. Croguennec
on the thermostability of basic proteins. Macromol Biosci 3:210–215
120. Gummel J, Boue ´ F, Deme B et al (2006) Charge stoichiometry inside polyelectrolyte-protein
complexes: a direct SANS measurement for the PSSNa-lysozyme system. J Phys Chem B
110:24837–24846
121. Chen YM, Yu CJ, Cheng TL et al (2008) Colorimetric detection of lysozyme based on
electrostatic interaction with human serum albumin-modified gold nanoparticles. Langmuir
24:3654–3660
122. Bayraktar H, Srivastava S, You C et al (2008) Controlled nanoparticle assembly through
protein conformational changes. Soft Matter 4:629–904
123. Liu Y, Guo R (2007) Interaction between casein and the oppositely charged surfactant.
Biomacromolecules 8:2902–2908
124. De M, Miranda OR, Rana S et al (2009) Size and geometry dependent protein–nanoparticle
self-assembly. Chem Commun 2009(16):2157–2159
125. Morfin I, Buhler E, Cousin F et al (2011) Rodlike complexes of a polyelectrolyte
(hyaluronan) and a protein (lysozyme) observed by SANS. Biomacromolecules 12:859–870
126. Mu ¨ller M, Ouyang W, Bohata K et al (2010) Nanostructured complexes of polyelectrolytes
and charged polypeptides. Adv Eng Mater 12:B519–B528
127. Mengarelli V, Auvray L, Zeghal M (2009) Phase behaviour and structure of stable complexes
of oppositely charged polyelectrolytes. Eur Phys Lett 85:58001
128. Matsudomi N, Yamamura Y, Kobayashi K (1987) Agregation between lysozyme and heatdenatured ovalbumin. Agric Biol Chem 51(7):1811–1817
129. Howell N, Yeboah N, Lewis D (1995) Studies on the electrostatic interactions of lysozyme
with α-lactalbumin and β-lactoglobulin. Int J Food Sci Technol 30:813–824
130. Biesheuvel PM, Lindhoud S, de Vries R et al (2006) Phase behavior of mixtures of oppositely
charged nanoparticles: heterogeneous Poisson-Boltzmann cell model applied to lysozyme
and succinylated lysozyme. Langmuir 22:1291–1300
131. Biesheuvel PM, Lindhoud S, Cohen Stuart MA et al (2006) Phase behavior of mixtures
of oppositely charged protein nanoparticles at asymmetric charge ratios. Phys Rev E
73(4):041408
132. Anema SG, de Kruif CG (2012) Co-acervates of lactoferrin and caseins. Soft Matter
8(16):4471–4478
133. Tiwari A, Bindal S, Bohidar HB (2009) Kinetics of protein-protein complex coacervation and
biphasic release of salbutamol sulfate from coacervate matrix. Biomacromolecules
10:184–189
134. Nigen M, Croguennec T, Madec MN et al (2007) Apo alpha-lactalbumin and lysozyme are
colocalized in their subsequently formed spherical supramolecular assembly. FEBS J
274:6085–6093
135. Nigen M, Le Tilly V, Croguennec T et al (2009) Molecular interaction between apo or holo αlactalbumin and lysozyme: formation of heterodimers as assessed by fluorescence
measurements. Biochim Biophys Acta 1794:709–715
136. Salvatore D, Duraffourg N, Favier A et al (2011) Investigation at residue level of the early
steps during the assembly of two proteins into supramolecular objects. Biomacromolecules
12(6):2200–2210
137. Shinoda W, DeVane R, Klein ML (2012) Computer simulation studies of self-assembling
macromolecules. Curr Opin Struct Biol 22:1–12
138. Salvatore D, Croguennec T, Bouhallab S et al (2011) Kinetics and structure during self-assembly
of oppositely charged proteins in aqueous solution. Biomacromolecules 12(5):1920–6192
139. Persson BA, Lund M (2009) Association and electrostatic steering of α-lactalbumin–
lysozyme heterodimers. Phys Chem Chem Phys 11:8879–8885
140. Xu Y, Mazzawi M, Chen K et al (2011) Protein purification by polyelectrolyte coacervation:
influence of protein charge anisotropy on selectivity. Biomacromolecules 12:1512–1522
100
S. Bouhallab and T. Croguennec