b
a
0.0
05
1.0
1.5
Abs (490nm)
0.0
0.5
1.0
1.5
Abs (490nm)
Phages 10 10 /ml
Phages 10 10 /ml
1.0
2.0
1.5
0.5
1.0
2.0
1.5
0.5
Fig. 12 (a, b) Efficiency of the panning procedures. Different phage libraries, C6 and L15, were
used in both a tube biopanning and a chromatopanning procedure. In both procedures, three rounds
were performed as described in [63]. The binding efficiency of the obtained phages was determined using phage ELISA (for experimental details see [63]). (a) Biopanning with a C6 library.
(b) Biopanning with a L15 library. Tube panning: round 1 ( filled square), round 3 (open square);
column panning: round 1 ( filled circle-dotted line). (Reproduced from [63] with permission)
Fig. 13 Flow resistance of columns packed with Sephadex G-100 (circles), Sephacryl S-1000 SF
(squares) and anion-exchange cryogels (diamonds). The results are presented as measured flow
rate versus the set-up flow rate determined in a separate experiment when the pump settings were
calibrated against flow rate with no column connected. Supermacroporous monolithic cryogels
were prepared in a 5 mL syringe at À12
C. (Reproduced from [65] with permission)
262
B. Mattiasson