3.2 Approach for the Development of Analytical Procedure …
47
b d = c/m and b s = b d /(m − 1) = c/{m(m − 1)}
b t =
c
(m − 1)
Although ‘θ c ’ represents the solid retention time for the suspended biomass, it
becomes p * θ in a semi-batch reactor provided with no recirculation. In this case,
also the slope and intercept of the ‘best-fit line’ considerably influence the values of
b s and b d .
3.3 Practice of Aeration in the Reactor
The aeration facility has been provided in the reactor with air compressor along
with controlled airflow through airflow meter with an average airflow of 20–30 l/m.
The airflow should be uniformly distributed through air nozzles to different slots of
attached media, i.e., through nine (9) number slots in octagonal attachment media
used in our experiment. Mechanical mixing with impeller along with aeration facility
maintains proper agitation in order to make availability of suspended MLSS in the
aeration tank for the biological activity. The DO concentration maintained in the
reactor is between 0.5 and 1 mg/l. Diffused aeration is employed with submerged
course diffusers.
3.4 Sampling Procedure and Preparation of Sample
3.4.1 Sampling Procedure
The hybrid bioreactor was run by both synthetic municipal wastewater and raw
municipal wastewater under continuous mode. In case of synthetic municipal wastewater, test was run with three variable initial COD concentrations, viz. 150, 200 and
250 mg/l and maintaining a HRT of 4, 6 and 8 h. The initial biomass concentration
(both suspended and attached) was also varied as per initial COD concentration.
The reactor was allowed to run by means of peristaltic pump until quasi-steady-state
condition is reached. During the continuous study, the effluent sample was taken from
the overflow of the secondary clarifier at an interval of three (3) h for measuring the
final COD concentration of its soluble sample. The reactor content was taken for
determining suspended biomass concentration at both initial and final conditions. A
portion of attached media (a detachable perspex sheet fin) was washed out with 1 N
NaOH solution to measure the attached biomass.
47
b d = c/m and b s = b d /(m − 1) = c/{m(m − 1)}
b t =
c
(m − 1)
Although ‘θ c ’ represents the solid retention time for the suspended biomass, it
becomes p * θ in a semi-batch reactor provided with no recirculation. In this case,
also the slope and intercept of the ‘best-fit line’ considerably influence the values of
b s and b d .
3.3 Practice of Aeration in the Reactor
The aeration facility has been provided in the reactor with air compressor along
with controlled airflow through airflow meter with an average airflow of 20–30 l/m.
The airflow should be uniformly distributed through air nozzles to different slots of
attached media, i.e., through nine (9) number slots in octagonal attachment media
used in our experiment. Mechanical mixing with impeller along with aeration facility
maintains proper agitation in order to make availability of suspended MLSS in the
aeration tank for the biological activity. The DO concentration maintained in the
reactor is between 0.5 and 1 mg/l. Diffused aeration is employed with submerged
course diffusers.
3.4 Sampling Procedure and Preparation of Sample
3.4.1 Sampling Procedure
The hybrid bioreactor was run by both synthetic municipal wastewater and raw
municipal wastewater under continuous mode. In case of synthetic municipal wastewater, test was run with three variable initial COD concentrations, viz. 150, 200 and
250 mg/l and maintaining a HRT of 4, 6 and 8 h. The initial biomass concentration
(both suspended and attached) was also varied as per initial COD concentration.
The reactor was allowed to run by means of peristaltic pump until quasi-steady-state
condition is reached. During the continuous study, the effluent sample was taken from
the overflow of the secondary clarifier at an interval of three (3) h for measuring the
final COD concentration of its soluble sample. The reactor content was taken for
determining suspended biomass concentration at both initial and final conditions. A
portion of attached media (a detachable perspex sheet fin) was washed out with 1 N
NaOH solution to measure the attached biomass.