6.3 Details of Continuous Study
93
put into a desiccator and its final weight was taken. Thus, it is possible to calculate
the MLSS by subtracting the initial weight from the final one.
Biomass density in the attached media was determined by washing the biofilm
surface with 25 mL 0.1 (N) NaOH and then heating the solution at 80 °C [1, 2].
Subsequently, by measuring the protein concentration in the washed out content as
per Lowry’s method [3] the amount of attached biomass was estimated.
The results of continuous study with synthetic wastewater are tabulated in Table
6.3.
Table 6.3 Continuous study with synthetic wastewater sample
Run No.
HR
MLSS
INITIAL
F/M
Effluent
% age
Loading
01
4
2500
150
0.1464
89
41
0.9
02
3000
0.15
75
50
0.9
03
3500
0.163
55
63
0.9
04
6
2500
0.168
42
70
0.6
05
3000
0.147
40
73
0.6
06
3500
0.128
38
75
0.6
07
8
2500
0.15
25
83
0.45
08
3000
0.13
20
87
0.45
09
3500
0.114
17
89
0.45
10
4
2500
200
0.26
92
54
1.2
11
3000
0.22
89
56
1.2
12
3500
0.238
61
70
1.2
13
6
2500
0.24
43
75
0.8
14
3000
0.207
45
78
0.8
15
3500
0.184
39
81
0.8
16
8
2500
0.2076
27
87
0.6
17
3000
0.179
21
90
0.6
18
3500
0.156
18
91
0.6
19
4
2500
250
0.36
100
60
1.5
20
3000
0.324
88
65
1.5
21
3500
0.31
69
72
1.5
22
6
2500
0.312
38
78
1
23
3000
0.268
49
80
1
24
3500
0.234
45
82
1
25
8
2500
0.264
28
88
0.75
26
3000
0.225
25
90
0.75
27
3500
0.197
20
92
0.75