Fungal Production of Single Cell Oil Using Defatted Oilseed …
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(16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and some amounts of linolenic
(18:3) acid.
As the nitrogen, mineral sources, and nutrient content varied, they trigger the
production of fatty acids in various percentages. The result showed that medium
I helped to produce oleic acid in A. niger, whereas, more amount of palmitic and
linoleic acids were found in A. heteromorphus and A. fumigatus and Medium II
produced highest amount of oleic acid in A. heteromorphus and A. fumigatus and a
little amount of linolenic acid was produced in A. niger. But the most common and
abundant fatty acids extracted were palmitic (C16:0), stearic (C18:0), oleic (C18:1),
and linoleic (C18:2), which often corresponded to 95% of the Aspergillus sp. total
FA content (Fraga et al. 2008). These fatty acid profiles can be used as reliable
biomarkers for these cultures (Devi et al. 2006).
3.4 Effect of Incubation Time on Biomass and Lipid
Production by the Fungal Isolates
It was observed that both biomass production and their corresponding lipid production increased simultaneously up to the 7th day of incubation and then went lower
as day passed, whereas glucose exhaust day by day for lipid production. After 7th
day, glucose concentration in the media became limited, resulting in deterioration in
biomass and lipid production. Similar kind of growth curve was noted in Aspergillus
oryzae on PDA, having optimal harvesting time of 5 days (Pinasthika et al. 2018)
(Table 3).
3.5 Growth and Lipid Production by the Isolated Fungus
The growth yield efficiency, lipid productivity, and lipid coefficient with their
corresponding sugar consumption by the fungus are shown in Table 4.
The Y L/C values of the fungi were ranging from 6 to 11 g lipids/100 g of glucose
consumed. In a similar study, Y L/C values of fungal isolates were ranged from 4.7
to 14.0 g lipids/100 g of glucose consumed. The higher Y L/C values demonstrate the
higher substrate conversion efficiency of the isolated oleaginous fungi (Muniraj et al.
2017).
3.6 Proximate Analysis of Defatted Oilseed Meal
Total carbohydrate, crude protein, moisture content, ash, and fiber content were
measured after total extraction of oil from flaxseed, black mustard seed, and rice
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(16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and some amounts of linolenic
(18:3) acid.
As the nitrogen, mineral sources, and nutrient content varied, they trigger the
production of fatty acids in various percentages. The result showed that medium
I helped to produce oleic acid in A. niger, whereas, more amount of palmitic and
linoleic acids were found in A. heteromorphus and A. fumigatus and Medium II
produced highest amount of oleic acid in A. heteromorphus and A. fumigatus and a
little amount of linolenic acid was produced in A. niger. But the most common and
abundant fatty acids extracted were palmitic (C16:0), stearic (C18:0), oleic (C18:1),
and linoleic (C18:2), which often corresponded to 95% of the Aspergillus sp. total
FA content (Fraga et al. 2008). These fatty acid profiles can be used as reliable
biomarkers for these cultures (Devi et al. 2006).
3.4 Effect of Incubation Time on Biomass and Lipid
Production by the Fungal Isolates
It was observed that both biomass production and their corresponding lipid production increased simultaneously up to the 7th day of incubation and then went lower
as day passed, whereas glucose exhaust day by day for lipid production. After 7th
day, glucose concentration in the media became limited, resulting in deterioration in
biomass and lipid production. Similar kind of growth curve was noted in Aspergillus
oryzae on PDA, having optimal harvesting time of 5 days (Pinasthika et al. 2018)
(Table 3).
3.5 Growth and Lipid Production by the Isolated Fungus
The growth yield efficiency, lipid productivity, and lipid coefficient with their
corresponding sugar consumption by the fungus are shown in Table 4.
The Y L/C values of the fungi were ranging from 6 to 11 g lipids/100 g of glucose
consumed. In a similar study, Y L/C values of fungal isolates were ranged from 4.7
to 14.0 g lipids/100 g of glucose consumed. The higher Y L/C values demonstrate the
higher substrate conversion efficiency of the isolated oleaginous fungi (Muniraj et al.
2017).
3.6 Proximate Analysis of Defatted Oilseed Meal
Total carbohydrate, crude protein, moisture content, ash, and fiber content were
measured after total extraction of oil from flaxseed, black mustard seed, and rice