Anti-adhering Property Study of Green
Nanotechnology Based Modified Chitin
Flakes: A Novel Approach Towards
Biofilm Eradication
Anindita Chatterjee, Rimashree Baishya, and Soma Banerjee
Abstract Biofilms are complex, heterogeneous, integrated community of surfaceattached microorganisms that remain encased within the self-producing extracellular polymeric matrix. In healthcare-associated infections, biofilms are linked to
the survival of pathogenic bacteria—Staphylococcus aureus, showing antimicrobial
resistance. The present work focuses on the anti-adhering property study of silvercoated green nanoparticles against biofilm-forming bacterial population of Staphylococcus aureus (ATCC 25923) on modified chitin model. The UV spectroscopy
was used to monitor the formation of silver nanoparticles from Curcuma longa and
Azadirachta indica extracts. The bacteria were seeded in TSB media with chitin as
the substrate for the biofilm formation. Biofilm inhibition on chitin flakes coated with
green nanoparticles and antibiotics (Vancomycin and Gentamycin) were studied and
cell counts were observed at intervals from 24 h to 96 h. Morphology of the biofilm
architecture on coated chitins was analyzed by SEM and the change in the chemical
components was observed by FTIR. The results show the reduction of microbial
adhesion by 81% at 24 h and 90–96% at 96 h when chitin flakes are coated with
green nanoparticles.
Keywords Silver nanoparticles · Green nanotechnology · Biofilm · Chitin flakes ·
Anti-adhering activity
A. Chatterjee · R. Baishya · S. Banerjee (B)
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Chowbaga, Kolkata,
West Bengal 700107, India
e-mail: somabanerjee2005@gmail.com
A. Chatterjee
e-mail: aninditachatterjee.91@gmail.com
R. Baishya
e-mail: reemaorbabu@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_49
485
Nanotechnology Based Modified Chitin
Flakes: A Novel Approach Towards
Biofilm Eradication
Anindita Chatterjee, Rimashree Baishya, and Soma Banerjee
Abstract Biofilms are complex, heterogeneous, integrated community of surfaceattached microorganisms that remain encased within the self-producing extracellular polymeric matrix. In healthcare-associated infections, biofilms are linked to
the survival of pathogenic bacteria—Staphylococcus aureus, showing antimicrobial
resistance. The present work focuses on the anti-adhering property study of silvercoated green nanoparticles against biofilm-forming bacterial population of Staphylococcus aureus (ATCC 25923) on modified chitin model. The UV spectroscopy
was used to monitor the formation of silver nanoparticles from Curcuma longa and
Azadirachta indica extracts. The bacteria were seeded in TSB media with chitin as
the substrate for the biofilm formation. Biofilm inhibition on chitin flakes coated with
green nanoparticles and antibiotics (Vancomycin and Gentamycin) were studied and
cell counts were observed at intervals from 24 h to 96 h. Morphology of the biofilm
architecture on coated chitins was analyzed by SEM and the change in the chemical
components was observed by FTIR. The results show the reduction of microbial
adhesion by 81% at 24 h and 90–96% at 96 h when chitin flakes are coated with
green nanoparticles.
Keywords Silver nanoparticles · Green nanotechnology · Biofilm · Chitin flakes ·
Anti-adhering activity
A. Chatterjee · R. Baishya · S. Banerjee (B)
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Chowbaga, Kolkata,
West Bengal 700107, India
e-mail: somabanerjee2005@gmail.com
A. Chatterjee
e-mail: aninditachatterjee.91@gmail.com
R. Baishya
e-mail: reemaorbabu@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_49
485