Wound Healing and Antimicrobial
Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark
Extract on Diabetic Wound In Mice
Baishakhi Bairagi and Debjani Nath
Abstract The phytochemicals can act as reducing agents as well as nontoxic capping
materials of metal nanosilver. The objective of the present work was to fabricate
the synthesis of silver nanoparticle using aqueous bark extract of Saraca asoca as
reducing agent and testing the wound healing efficacy of the material by studying
the level of wound contraction and re-epithelialization and expression of inflammatory factors like cytokines and different proinflammatory markers in diabetic mice.
The biocompatibility of the synthesized silver nanoparticle was tested by cell cycle
analysis, ROS generation, DAPI, comet assay. It was indicated that the synthesized
silver nanoparticles were nontoxic to normal mice in vivo. Antibacterial properties
of the silver formulation are comparable to different antibiotics and more effective
against Bacillus firmus and Staphylococcus gallinarum of diabetic wound. SEM
images and histopathological observations revealed that the healing process was
accelerated in the silver nanoparticle treated group. The significant healing efficacy
of phytofabricated nanoparticles was evident by the observations of downregulation
of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, and upregulation of
anti-inflammatory cytokine, IL-10.
Keywords Saraca asoca · Silver nanoparticles · Diabetic wound · Wound
healing · Antibacterial efficacy
1 Introduction
Diabetic wound is a challenging problem due to its delayed process of healing that
needs a longer time for proper cure. Silver nanoparticles are best studied due to
their antimicrobial activity and have the efficacy in bypassing mechanisms of drug
B. Bairagi · D. Nath (B)
Department of Zoology, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India
e-mail: nath_debjani@yahoo.co.in
B. Bairagi
e-mail: baisakhi91@yahoo.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_48
475
Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark
Extract on Diabetic Wound In Mice
Baishakhi Bairagi and Debjani Nath
Abstract The phytochemicals can act as reducing agents as well as nontoxic capping
materials of metal nanosilver. The objective of the present work was to fabricate
the synthesis of silver nanoparticle using aqueous bark extract of Saraca asoca as
reducing agent and testing the wound healing efficacy of the material by studying
the level of wound contraction and re-epithelialization and expression of inflammatory factors like cytokines and different proinflammatory markers in diabetic mice.
The biocompatibility of the synthesized silver nanoparticle was tested by cell cycle
analysis, ROS generation, DAPI, comet assay. It was indicated that the synthesized
silver nanoparticles were nontoxic to normal mice in vivo. Antibacterial properties
of the silver formulation are comparable to different antibiotics and more effective
against Bacillus firmus and Staphylococcus gallinarum of diabetic wound. SEM
images and histopathological observations revealed that the healing process was
accelerated in the silver nanoparticle treated group. The significant healing efficacy
of phytofabricated nanoparticles was evident by the observations of downregulation
of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, and upregulation of
anti-inflammatory cytokine, IL-10.
Keywords Saraca asoca · Silver nanoparticles · Diabetic wound · Wound
healing · Antibacterial efficacy
1 Introduction
Diabetic wound is a challenging problem due to its delayed process of healing that
needs a longer time for proper cure. Silver nanoparticles are best studied due to
their antimicrobial activity and have the efficacy in bypassing mechanisms of drug
B. Bairagi · D. Nath (B)
Department of Zoology, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India
e-mail: nath_debjani@yahoo.co.in
B. Bairagi
e-mail: baisakhi91@yahoo.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_48
475