Utilization of Low-Cost Fatty Acid
Sources by Bacterial Isolate for Improved
Production of Valuable Prodigiosin
Dalia Dasgupta Mandal, Subhasree Majumdar, Sovan Dey, Sourav Dutta,
and Tamal Mandal
Abstract Prodigiosin, a bright pinkish-red bioactive compound, has enormous pharmacological significance as anti-cancer, anti-microbial, anti-oxidant and immunosuppressive agents. This is produced by several bacterial species such as Serratia,
Pseudomonas and Streptomyces. It is one of the most conspicuous bacterial pigments
extant in the microbial world. In the present work, cost-effective production of prodigiosin from Serratia marcescens NITDPER1 an indigenous bacterial strain isolated
from paper mill effluent was studied. The pigment yield from this isolate was much
less in nutrient broth media and peptone glycerol media 0.047 ± 0.001 g L
−1 and
0.02 ± 0.003 g L
−1 , respectively, at 30 °C, pH 7 and 120 rpm. To increase production, fatty acid as carbon source, such as peanut oil, peanut powder and palm oil,
was used that gave better yield compared with common media. Among these three,
peanut oil gave the highest yield (5.36 ± 0.81 g L
−1 ) followed by palm oil (4.43
± 0.72 g L
−1 ) and peanut powder (3 ± 0.45 g L
−1 ). From economical perspectives, palm oil as a low-cost fatty acid source showed noticeable production and
D. Dasgupta Mandal (B) · S. Dey · S. Dutta
Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi
Avenue, Durgapur, West Bengal 713209, India
e-mail: dalia.dasgupta@bt.nitdgp.ac.in
S. Dey
e-mail: deysovan97@gmail.com
S. Dutta
e-mail: kittu.sd79@gmail.com
S. Majumdar
Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, India
e-mail: subhasree.majumdar1987@gmail.com
Department of Zoology, Sonamukhi College, Sonamukhi, Bankura, West Bengal 722207, India
T. Mandal
Department of Chemical Engineering, National Institute of Technology Durgapur, Mahatma
Gandhi Avenue, Durgapur, West Bengal 713209, India
e-mail: tamal.mandal@che.nitdgp.ac.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_3
21
Sources by Bacterial Isolate for Improved
Production of Valuable Prodigiosin
Dalia Dasgupta Mandal, Subhasree Majumdar, Sovan Dey, Sourav Dutta,
and Tamal Mandal
Abstract Prodigiosin, a bright pinkish-red bioactive compound, has enormous pharmacological significance as anti-cancer, anti-microbial, anti-oxidant and immunosuppressive agents. This is produced by several bacterial species such as Serratia,
Pseudomonas and Streptomyces. It is one of the most conspicuous bacterial pigments
extant in the microbial world. In the present work, cost-effective production of prodigiosin from Serratia marcescens NITDPER1 an indigenous bacterial strain isolated
from paper mill effluent was studied. The pigment yield from this isolate was much
less in nutrient broth media and peptone glycerol media 0.047 ± 0.001 g L
−1 and
0.02 ± 0.003 g L
−1 , respectively, at 30 °C, pH 7 and 120 rpm. To increase production, fatty acid as carbon source, such as peanut oil, peanut powder and palm oil,
was used that gave better yield compared with common media. Among these three,
peanut oil gave the highest yield (5.36 ± 0.81 g L
−1 ) followed by palm oil (4.43
± 0.72 g L
−1 ) and peanut powder (3 ± 0.45 g L
−1 ). From economical perspectives, palm oil as a low-cost fatty acid source showed noticeable production and
D. Dasgupta Mandal (B) · S. Dey · S. Dutta
Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi
Avenue, Durgapur, West Bengal 713209, India
e-mail: dalia.dasgupta@bt.nitdgp.ac.in
S. Dey
e-mail: deysovan97@gmail.com
S. Dutta
e-mail: kittu.sd79@gmail.com
S. Majumdar
Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, India
e-mail: subhasree.majumdar1987@gmail.com
Department of Zoology, Sonamukhi College, Sonamukhi, Bankura, West Bengal 722207, India
T. Mandal
Department of Chemical Engineering, National Institute of Technology Durgapur, Mahatma
Gandhi Avenue, Durgapur, West Bengal 713209, India
e-mail: tamal.mandal@che.nitdgp.ac.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_3
21