Synthesis and Characterization of TiO 2
Nanoparticle and Checking Its
Antimicrobial Activity Against
Escherichia coli and Staphylococcus
aureus
Ram Singh Purty, Ishant Shakya, and Sayan Chatterjee
Abstract The aim of the experiment was to produce the conjugated nanoparticles
and optimize its production that can be replicated at industrial level. The primarily
used chemical from which TiO 2 nanoparticle was made is titanium isopropoxide
(C 12 H 28 O 4 Ti). The primary dopant used was urea CO(NH 2 ) 2 . Ammonium chloride
NH 4 Cl was used as a secondary nitrogen dopant. Different molar ratios of these
dopants were used while synthesizing the nanoparticle. The nanoparticle synthesized
was characterized by the help of UV–vis spectrophotometer and DLS (dynamic light
scattering). The synthesized NP was able to degrade the bromothymol blue dye under
the action of UV received by the direct sunlight proving its photocatalytic property.
The nanoparticles were tested for its antimicrobial property on the gram-negative
bacteria Escherichia coli and gram-positive bacteria Staphylococcus aureus. Broth
dilution method was used to study the antimicrobial effect. The results were further
confirmed with the help of agar diffusion assay. The synthesized nanoparticle was
able to inhibit the growth of the bacteria in both the assays successfully.
Keywords TiO 2 nanoparticle · Antimicrobial activity · Escherichia coli ·
Staphylococcus aureus · Synthesis of nanoparticle · Characterisation
1 Introduction
Nanotechnology is the technology that is aimed at synthesizing and getting new products that have some novel functionality. This in particular is the new branch of study
R. S. Purty · I. Shakya · S. Chatterjee (B)
University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi,
India
e-mail: sayan@ipu.ac.in
R. S. Purty
e-mail: rspurty@ipu.ac.in
I. Shakya
e-mail: shakyaishant97@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_32
317
Nanoparticle and Checking Its
Antimicrobial Activity Against
Escherichia coli and Staphylococcus
aureus
Ram Singh Purty, Ishant Shakya, and Sayan Chatterjee
Abstract The aim of the experiment was to produce the conjugated nanoparticles
and optimize its production that can be replicated at industrial level. The primarily
used chemical from which TiO 2 nanoparticle was made is titanium isopropoxide
(C 12 H 28 O 4 Ti). The primary dopant used was urea CO(NH 2 ) 2 . Ammonium chloride
NH 4 Cl was used as a secondary nitrogen dopant. Different molar ratios of these
dopants were used while synthesizing the nanoparticle. The nanoparticle synthesized
was characterized by the help of UV–vis spectrophotometer and DLS (dynamic light
scattering). The synthesized NP was able to degrade the bromothymol blue dye under
the action of UV received by the direct sunlight proving its photocatalytic property.
The nanoparticles were tested for its antimicrobial property on the gram-negative
bacteria Escherichia coli and gram-positive bacteria Staphylococcus aureus. Broth
dilution method was used to study the antimicrobial effect. The results were further
confirmed with the help of agar diffusion assay. The synthesized nanoparticle was
able to inhibit the growth of the bacteria in both the assays successfully.
Keywords TiO 2 nanoparticle · Antimicrobial activity · Escherichia coli ·
Staphylococcus aureus · Synthesis of nanoparticle · Characterisation
1 Introduction
Nanotechnology is the technology that is aimed at synthesizing and getting new products that have some novel functionality. This in particular is the new branch of study
R. S. Purty · I. Shakya · S. Chatterjee (B)
University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi,
India
e-mail: sayan@ipu.ac.in
R. S. Purty
e-mail: rspurty@ipu.ac.in
I. Shakya
e-mail: shakyaishant97@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_32
317