Effect of Calcium Salts on Salinity Stress on Morphology …
309
Fig. 2 Comparative Shoot
growth
0
5
10
15
20
Shoot length in cm
Fig. 3 Reducing Sugar
content
0
0.5
1
1.5
2
2.5
3
3.5
Reducing Sugar
content(mg/g)
In seedlings, shoot growth was decreased 55%, 49% and 55% in CN2, PAT and
DDH variety, respectively, in 200 mM NaCl with respect to control (Fig. 2). The
results show that the maximum recovery of shoot growth was found in 15 mM
CaSO 4 treatment set and 32% in CN2 variety, with respect to the seedlings stressed
only with 200 mM NaCl; whereas, the shoot growth of DDH variety was markedly
restored in 10 mM CaCO 3 set (1.2 times). This result matches the findings of Lutts
et al. (1996) and Tahjib et al. (2018), where salinity decreased growth of rice leaves
and they have also found that shoot length can increase through supplementation
with CaCl 2 to the salt-treated seedlings. Ornami and Hammes (2006) have found
that CaSO 4 showed stronger ameliorative effect than CaCl 2 during the growth rate
study of salt-stressed amaranth. Although Bassett (1980) has found that CaCO 3 has
an ameliorative effect on salt-stressed Bromus mollis (Fig. 3).
4.2 Proline Content
Proline accumulation (Fig. 4) was markedly increased in CN2 (10 times), PAT (3.1
times) and DDH (4.6 times) variety in 200 mM NaCl set. The highest reduction in
proline accumulation was found in the treatment set of 10 mM CaSO 4 (81%), 15 mM
CaCO 3 (73%) and 5 mM CaCO 3 (98%) in CN2, PAT and DDH variety, respectively,
309
Fig. 2 Comparative Shoot
growth
0
5
10
15
20
Shoot length in cm
Fig. 3 Reducing Sugar
content
0
0.5
1
1.5
2
2.5
3
3.5
Reducing Sugar
content(mg/g)
In seedlings, shoot growth was decreased 55%, 49% and 55% in CN2, PAT and
DDH variety, respectively, in 200 mM NaCl with respect to control (Fig. 2). The
results show that the maximum recovery of shoot growth was found in 15 mM
CaSO 4 treatment set and 32% in CN2 variety, with respect to the seedlings stressed
only with 200 mM NaCl; whereas, the shoot growth of DDH variety was markedly
restored in 10 mM CaCO 3 set (1.2 times). This result matches the findings of Lutts
et al. (1996) and Tahjib et al. (2018), where salinity decreased growth of rice leaves
and they have also found that shoot length can increase through supplementation
with CaCl 2 to the salt-treated seedlings. Ornami and Hammes (2006) have found
that CaSO 4 showed stronger ameliorative effect than CaCl 2 during the growth rate
study of salt-stressed amaranth. Although Bassett (1980) has found that CaCO 3 has
an ameliorative effect on salt-stressed Bromus mollis (Fig. 3).
4.2 Proline Content
Proline accumulation (Fig. 4) was markedly increased in CN2 (10 times), PAT (3.1
times) and DDH (4.6 times) variety in 200 mM NaCl set. The highest reduction in
proline accumulation was found in the treatment set of 10 mM CaSO 4 (81%), 15 mM
CaCO 3 (73%) and 5 mM CaCO 3 (98%) in CN2, PAT and DDH variety, respectively,