Effect of Calcium Salts on Salinity Stress
on Morphology and Biochemical
Estimation of Rice Seedlings
Banhishikha Singh and Soma Banerjee
Abstract Soil salinity is one of the major abiotic stresses affecting plant growth
and productivity globally as well in the Sunderban area of West Bengal. The study
comprises comparison of the effect of the combined action of sodium chloride
(NaCl) and three types of calcium salts calcium chloride (CaCl 2 ), calcium sulphate
(CaSO 4 ) and calcium carbonate (CaCO 3 ) on three rice varieties (‘Chinsurah Nona2’, ‘Dudherswar’ and ‘Pratiksha’) both morphological and biochemical estimations,
i.e. shoot growth, chlorophyll, proline, starch and reducing sugar content and antioxidants expressions. The results show maximum recovery of shoot growth in 15 mMCaSO 4 with 200 mM NaCl treatment set with respect to seedlings stressed with
only 200 mM NaCl, i.e. 32% in variety ‘Chinsurah Nona-2’ and 10.5% in variety
‘Pratiksha’, whereas ‘Dudherswar’ variety shows 1.2 times recovery in 10 mMCaCO 3 set. In case of chlorophyll content in the following sets, 5 mM CaCl 2 , 15 mM
CaSO 4 and 10 mM CaCO 3 are showing best recovery. Similarly, for proline, starch
and reducing sugar content, 10 mM CaSO 4 and 15 mM CaCO 3 sets show best results
against 200 mM NaCl. For antioxidant expression in ‘Chinsurah Nona-2’ variety,
the catalase activity increased 4.7 times in 5 mM CaCO 3 set followed by three times
increase in ascorbate peroxidase activity in 10 mM CaCO 3 , and for lipid peroxidation, 79% decrease observed in 10 mM CaCl 2 . In ‘Pratiksha’ variety, 28% increase
in catalase activity in 5 mM CaCO 3 set and 86% decrease in ascorbate peroxidase
activity in 15 mM CaSO 4 , 1.4 times increase in glutathione reductase activity, 5 mM
CaCO 3 and lipid peroxidation activity showed 59% decrease in 10 mM CaSO 4 .
‘Dudherswar’ variety showed marked response in all the antioxidant estimations
in comparison with the other varieties, 5.5 times increase in superoxide dismutase
activity in 15 mM CaCl 2 set, 11 times increase in catalase activity in 5 mM CaSO 4 ,
89% increase in ascorbate peroxidase activity in 5 mM CaCO 3 the glutathione reductase activity highest increased (103%) in 10 mM CaCl 2 and 65% decrease in lipid
B. Singh · S. Banerjee (B)
Department of Biotechnology, Heritage Institute of Technology, Kolkata, India
e-mail: soma.banerjee@heritageit.edu
B. Singh
e-mail: banhi.biotech@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_31
305
on Morphology and Biochemical
Estimation of Rice Seedlings
Banhishikha Singh and Soma Banerjee
Abstract Soil salinity is one of the major abiotic stresses affecting plant growth
and productivity globally as well in the Sunderban area of West Bengal. The study
comprises comparison of the effect of the combined action of sodium chloride
(NaCl) and three types of calcium salts calcium chloride (CaCl 2 ), calcium sulphate
(CaSO 4 ) and calcium carbonate (CaCO 3 ) on three rice varieties (‘Chinsurah Nona2’, ‘Dudherswar’ and ‘Pratiksha’) both morphological and biochemical estimations,
i.e. shoot growth, chlorophyll, proline, starch and reducing sugar content and antioxidants expressions. The results show maximum recovery of shoot growth in 15 mMCaSO 4 with 200 mM NaCl treatment set with respect to seedlings stressed with
only 200 mM NaCl, i.e. 32% in variety ‘Chinsurah Nona-2’ and 10.5% in variety
‘Pratiksha’, whereas ‘Dudherswar’ variety shows 1.2 times recovery in 10 mMCaCO 3 set. In case of chlorophyll content in the following sets, 5 mM CaCl 2 , 15 mM
CaSO 4 and 10 mM CaCO 3 are showing best recovery. Similarly, for proline, starch
and reducing sugar content, 10 mM CaSO 4 and 15 mM CaCO 3 sets show best results
against 200 mM NaCl. For antioxidant expression in ‘Chinsurah Nona-2’ variety,
the catalase activity increased 4.7 times in 5 mM CaCO 3 set followed by three times
increase in ascorbate peroxidase activity in 10 mM CaCO 3 , and for lipid peroxidation, 79% decrease observed in 10 mM CaCl 2 . In ‘Pratiksha’ variety, 28% increase
in catalase activity in 5 mM CaCO 3 set and 86% decrease in ascorbate peroxidase
activity in 15 mM CaSO 4 , 1.4 times increase in glutathione reductase activity, 5 mM
CaCO 3 and lipid peroxidation activity showed 59% decrease in 10 mM CaSO 4 .
‘Dudherswar’ variety showed marked response in all the antioxidant estimations
in comparison with the other varieties, 5.5 times increase in superoxide dismutase
activity in 15 mM CaCl 2 set, 11 times increase in catalase activity in 5 mM CaSO 4 ,
89% increase in ascorbate peroxidase activity in 5 mM CaCO 3 the glutathione reductase activity highest increased (103%) in 10 mM CaCl 2 and 65% decrease in lipid
B. Singh · S. Banerjee (B)
Department of Biotechnology, Heritage Institute of Technology, Kolkata, India
e-mail: soma.banerjee@heritageit.edu
B. Singh
e-mail: banhi.biotech@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_31
305