Value Addition of Agricultural Wastes for Improved Production …
275
Table 4 Saccharification yield of crude enzymes of fungal extracts produced on pretreated
cauliflower wastes by mono and co-culture in comparison to commercially available enzymes
Enzymes
Saccharification yield (%)
Os CS
Os CL
Crude cellulase A. niger
71.15 ± 1.58 a,d
69.87 ± 2.31 a,d
Crude cellulase Co culture
76.23 ± 0.78 a,d
70.87 ± 1.26 a,d
Commercial cellulase
70 ± 0.62 a,d
59.4 ± 0.78 a,d
Crude xylanase A. niger
68.16 ± 1.58
71.89 ± 2.31
Crude xylanase Co culture
75.61 ± 1.34
93.24 ± 1.27
Commercial xylanase
57.52 ± 0.56 a,d
87.62 ± 1.24 a,d
sugars and other value-added products. Table 4 gave the saccharification yields
achieved in the bioconversion of pretreated lignocellulosic substrates for production of fermentable sugars. Results here obtained clearly indicated higher saccharification efficiency was recorded with in-house-produced cellulases and xylanase on
pretreated waste biomasses than those of commercial cellulases and xylanase.
Os Organosolvent pretreatment, CS Cauliflower stalk, CL Cauliflower leaf Sample
evaluation is done in triplicate. Values are calculated as Mean ± SD(n = 3). Lowercase letters indicate significant differences (p ≤ 0.05). Values followed by the same
letter are not significantly different (p ≤ 0.05).
4 Conclusion
The present study highlighted the feasibility of utilization of cheap and readily available kitchen wastes as substrate for production of industrially important enzymes
under submerged fermentation, with a view to developing a low-cost production
system. Organosolvent treated cauliflower waste residues with the highest cellulose content induced maximum production of extracellular enzyme components in
comparison to untreated one. Compared to commercial lignocellulolytic enzymes,
the in-house enzymes from fungal extracts exhibited better hydrolytic efficiency
under the same hydrolytic condition.
Acknowledgements Authors are thankful to Indian Institute of Engineering Science and Technology, Shibpur (Formerly Bengal Engineering and Science University, Shibpur), West Bengal,
India for providing facilities for the completion of this research work. Research Scholar (S.
Majumdar) is grateful to UGC for providing financial support as Senior Research Fellowship.
275
Table 4 Saccharification yield of crude enzymes of fungal extracts produced on pretreated
cauliflower wastes by mono and co-culture in comparison to commercially available enzymes
Enzymes
Saccharification yield (%)
Os CS
Os CL
Crude cellulase A. niger
71.15 ± 1.58 a,d
69.87 ± 2.31 a,d
Crude cellulase Co culture
76.23 ± 0.78 a,d
70.87 ± 1.26 a,d
Commercial cellulase
70 ± 0.62 a,d
59.4 ± 0.78 a,d
Crude xylanase A. niger
68.16 ± 1.58
71.89 ± 2.31
Crude xylanase Co culture
75.61 ± 1.34
93.24 ± 1.27
Commercial xylanase
57.52 ± 0.56 a,d
87.62 ± 1.24 a,d
sugars and other value-added products. Table 4 gave the saccharification yields
achieved in the bioconversion of pretreated lignocellulosic substrates for production of fermentable sugars. Results here obtained clearly indicated higher saccharification efficiency was recorded with in-house-produced cellulases and xylanase on
pretreated waste biomasses than those of commercial cellulases and xylanase.
Os Organosolvent pretreatment, CS Cauliflower stalk, CL Cauliflower leaf Sample
evaluation is done in triplicate. Values are calculated as Mean ± SD(n = 3). Lowercase letters indicate significant differences (p ≤ 0.05). Values followed by the same
letter are not significantly different (p ≤ 0.05).
4 Conclusion
The present study highlighted the feasibility of utilization of cheap and readily available kitchen wastes as substrate for production of industrially important enzymes
under submerged fermentation, with a view to developing a low-cost production
system. Organosolvent treated cauliflower waste residues with the highest cellulose content induced maximum production of extracellular enzyme components in
comparison to untreated one. Compared to commercial lignocellulolytic enzymes,
the in-house enzymes from fungal extracts exhibited better hydrolytic efficiency
under the same hydrolytic condition.
Acknowledgements Authors are thankful to Indian Institute of Engineering Science and Technology, Shibpur (Formerly Bengal Engineering and Science University, Shibpur), West Bengal,
India for providing facilities for the completion of this research work. Research Scholar (S.
Majumdar) is grateful to UGC for providing financial support as Senior Research Fellowship.