8
S. Chatterjee et al.
Fig. 3 Carboxymethyl cellulase activity of six bacterial isolates using 3, 5-dinitrosalicylic acid
(DNS) assay. a CDB1, b CDB4, c CDB29, d CDB32, e CDB34, and f CDB37
CDB-4 < CDB-29 < CDB-34 < CDB-1 (Fig. 3). Among these two bacterial isolates,
CDB-1 and CDB-34 showed maximum cellulase production ability. Out of these
strains, CDB-1 was chosen for molecular characterization.
3.4 Molecular Identification of Cellulolytic Bacteria
The strain CDB-1 that showed maximum cellulose activity was further subjected to
molecular characterization. The 16S rRNA gene sequence obtained was compared
using BLASTN program, which showed the strain CDB-1 to be Streptomyces albogriseolus. Multiple sequence alignment analysis with all the closely related genera
showed 99–98% similarities at the sequence level. Phylogenetic tree analysis showed
that the strain CDB-1 is closely related to other Streptomyces species available in the
database (Fig. 4). Several actinomycetes are known to degrade cellulose (Saini et al.
2015).
3.5 Production of Bioethanol from Tissue Paper
The strain CDB-1 was used for its cellulolytic potential to degrade tissue paper to
obtain glucose as the product that was, for further, used for fermentation. The strain
Sacchromyces cerevisiae was used in the fermentation process for the conversion of
glucose to ethanol. The fermentation product was obtained from the broth that was
further confirmed by iodoform test (Fig. 5). After distillation of the fermented mash,
the percentage of alcohol in distillate was calculated to be 7.8%.
S. Chatterjee et al.
Fig. 3 Carboxymethyl cellulase activity of six bacterial isolates using 3, 5-dinitrosalicylic acid
(DNS) assay. a CDB1, b CDB4, c CDB29, d CDB32, e CDB34, and f CDB37
CDB-4 < CDB-29 < CDB-34 < CDB-1 (Fig. 3). Among these two bacterial isolates,
CDB-1 and CDB-34 showed maximum cellulase production ability. Out of these
strains, CDB-1 was chosen for molecular characterization.
3.4 Molecular Identification of Cellulolytic Bacteria
The strain CDB-1 that showed maximum cellulose activity was further subjected to
molecular characterization. The 16S rRNA gene sequence obtained was compared
using BLASTN program, which showed the strain CDB-1 to be Streptomyces albogriseolus. Multiple sequence alignment analysis with all the closely related genera
showed 99–98% similarities at the sequence level. Phylogenetic tree analysis showed
that the strain CDB-1 is closely related to other Streptomyces species available in the
database (Fig. 4). Several actinomycetes are known to degrade cellulose (Saini et al.
2015).
3.5 Production of Bioethanol from Tissue Paper
The strain CDB-1 was used for its cellulolytic potential to degrade tissue paper to
obtain glucose as the product that was, for further, used for fermentation. The strain
Sacchromyces cerevisiae was used in the fermentation process for the conversion of
glucose to ethanol. The fermentation product was obtained from the broth that was
further confirmed by iodoform test (Fig. 5). After distillation of the fermented mash,
the percentage of alcohol in distillate was calculated to be 7.8%.