Isolation of Cellulose-Degrading Bacteria and to Use Their …
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Fig. 1 Screening of cellulose-degrading bacteria from the soil sample using serial dilution on
carboxymethyl cellulose (CMC) agar medium. a Undiluted, b 10 −1 , c 10 −2 , and d 10 −3
Fig. 2 a, b Cellulolytic potential of selected six bacteria, namely, CDB1, CDB4, CDB29, CDB32,
CDB34, and CDB37, showing halo zones around the colony on CMC agar medium
condition was performed to obtain the isolated colonies (Fig. 1). A total of 37 isolated
colonies were selected and named as cellulose-degrading bacteria, CDB 1–37. Isolation of CDB was also reported from the other sources like guts of invertebrates (Gupta
et al. 2012), kitchen waste (Kaur 2012), and sheep rumen (Guder and Krishna 2019).
3.2 Cellulolytic Ability Assay
Cellulose degrading ability was analyzed by streaking the isolated bacterial strains
on Congo Red agar media. Based on the morphology, six morphologically distinct
bacterial isolates that were giving halo zones in CMC agar medium were selected,
namely, CDB1, CDB4, CDB29, CDB32, CDB34, and CDB37 (Fig. 2).
3.3 Carboxymethyl Cellulase (CMCase) Activity
Production of cellulase enzyme by six bacterial isolates was analyzed using the 3, 5dinitrosalicylic acid (DNS) method. The cellulase production ability of the bacterial
isolates in the production medium was in the following order, CDB-37 < CDB-32 <
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Fig. 1 Screening of cellulose-degrading bacteria from the soil sample using serial dilution on
carboxymethyl cellulose (CMC) agar medium. a Undiluted, b 10 −1 , c 10 −2 , and d 10 −3
Fig. 2 a, b Cellulolytic potential of selected six bacteria, namely, CDB1, CDB4, CDB29, CDB32,
CDB34, and CDB37, showing halo zones around the colony on CMC agar medium
condition was performed to obtain the isolated colonies (Fig. 1). A total of 37 isolated
colonies were selected and named as cellulose-degrading bacteria, CDB 1–37. Isolation of CDB was also reported from the other sources like guts of invertebrates (Gupta
et al. 2012), kitchen waste (Kaur 2012), and sheep rumen (Guder and Krishna 2019).
3.2 Cellulolytic Ability Assay
Cellulose degrading ability was analyzed by streaking the isolated bacterial strains
on Congo Red agar media. Based on the morphology, six morphologically distinct
bacterial isolates that were giving halo zones in CMC agar medium were selected,
namely, CDB1, CDB4, CDB29, CDB32, CDB34, and CDB37 (Fig. 2).
3.3 Carboxymethyl Cellulase (CMCase) Activity
Production of cellulase enzyme by six bacterial isolates was analyzed using the 3, 5dinitrosalicylic acid (DNS) method. The cellulase production ability of the bacterial
isolates in the production medium was in the following order, CDB-37 < CDB-32 <