206
B. Boro et al.
With significant spectrophotometric and microscopic observation in RBCs, MgO
NPs treatment is expected to yield a significant amount of hemolysis. However, there
was no significant amount of hemolysis observed in higher concentration of MgO
NPs. Nanoparticle can bind with the RBC membrane acting as a cementing material
to the erythrocytes which enhance the RBC membranes to become stronger against
the intracellular shock (Ghosh et al. 2013). But after binding the MgO nanoparticle
with the RBC membrane component, functionality of the blood cell is still unclear.
Because of surface characteristics, it may bind with the erythrocytes, thus preventing
cell breakage. But deformed cellular morphology, as observed in Fig. 5, may be a
clue to the abnormal functioning of the erythrocytes in MgO treated samples.
4 Conclusion
The present study reveals that after 24 h of treatment of MgO NPs to the RBCs
the deformity of blood cell is high in low concentration of MgO NPs and low in
high concentration of MgO NPs. But it is assumed that reduced incubation time may
cause less harm to the RBCs. Functionalization of the MgO NPs may also eradicate the harmfulness of the MgO NPs. Further study, involving functionalization of
MgO NPs with required bio-medically important bioactive molecules, may enhance
the usability of MgO NPs in biomedical and pharmaceutical field of study for its
advancement.
Acknowledgements Authors acknowledge the DBT sponsored Advanced Level Institutional
Biotech Hub (Phase-II), Pub Kamrup College for giving full facility to perform this experiment.
Thankfulness is also expressed in equal sincerity and spirit to the Principal of Pub Kamrup College.
Authors also acknowledge the medical professional for helping the study in collecting the blood
samples from human subjects.
Compliance with Ethical Standards
Conflicts of interest The authors declare that they have no conflict of interest.
Ethical approval All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Ethics Committee of Govt. Ayurvedic College,
Assam, India and with the 1964 Helsinki declaration and its later amendments or comparable ethical
standards.
Informed consent Informed consent was obtained from all individual participants included in the
study.
B. Boro et al.
With significant spectrophotometric and microscopic observation in RBCs, MgO
NPs treatment is expected to yield a significant amount of hemolysis. However, there
was no significant amount of hemolysis observed in higher concentration of MgO
NPs. Nanoparticle can bind with the RBC membrane acting as a cementing material
to the erythrocytes which enhance the RBC membranes to become stronger against
the intracellular shock (Ghosh et al. 2013). But after binding the MgO nanoparticle
with the RBC membrane component, functionality of the blood cell is still unclear.
Because of surface characteristics, it may bind with the erythrocytes, thus preventing
cell breakage. But deformed cellular morphology, as observed in Fig. 5, may be a
clue to the abnormal functioning of the erythrocytes in MgO treated samples.
4 Conclusion
The present study reveals that after 24 h of treatment of MgO NPs to the RBCs
the deformity of blood cell is high in low concentration of MgO NPs and low in
high concentration of MgO NPs. But it is assumed that reduced incubation time may
cause less harm to the RBCs. Functionalization of the MgO NPs may also eradicate the harmfulness of the MgO NPs. Further study, involving functionalization of
MgO NPs with required bio-medically important bioactive molecules, may enhance
the usability of MgO NPs in biomedical and pharmaceutical field of study for its
advancement.
Acknowledgements Authors acknowledge the DBT sponsored Advanced Level Institutional
Biotech Hub (Phase-II), Pub Kamrup College for giving full facility to perform this experiment.
Thankfulness is also expressed in equal sincerity and spirit to the Principal of Pub Kamrup College.
Authors also acknowledge the medical professional for helping the study in collecting the blood
samples from human subjects.
Compliance with Ethical Standards
Conflicts of interest The authors declare that they have no conflict of interest.
Ethical approval All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Ethics Committee of Govt. Ayurvedic College,
Assam, India and with the 1964 Helsinki declaration and its later amendments or comparable ethical
standards.
Informed consent Informed consent was obtained from all individual participants included in the
study.