166
A. Mukherjee
4 Conclusion
Mentha spicata seems to be a good source of acid stable invertase obtained from a
non-toxic medicinal plant. The crude concentrated enzyme extract can effectively
be used for the production of invert syrup and also as a useful and safe remedy for
sucrose intolerance.
References
Bracho GE, Whitaker JR (1990) Purification and partial characterization of potato (Solanum
tuberosum) invertase and its endogenous proteinaceous inhibitor. Plant Physiol 92(2):386–394
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of
protein utilizing the principle of protein dye binding. Anal Biochem 72(1–2):248–254
Güney M, Oral B, Karahanli N et al (2006) The effect of Mentha spicata Labiatae on uterine tissue
in rats. Toxicol Ind Health 22(8):343–348
Krishnan HB, Pueppke SG (1990) Cherry fruit invertase: partial purification, characterization and
activity during fruit development. J Plant Physiol 135(6):662–666
Kulshrestha S, Tyagi P, Sindhi V et al (2013) Invertase and its applications—a brief review. J Pharm
Res 7:792–797
Kumar V, Kural MR, Pereira BM et al (2008) Spearmint induced hypothalamic oxidative stress
and testicular anti-androgenicity in male rats—altered levels of gene expression, enzymes and
hormones. Food Chem Toxicol 46(12):3563–3570
Lee HS, Sturm A (1996) Purification and characterization of neutral and alkaline invertase from
carrot. Plant Physiol 112(4):1513–1522
Li R, Li J, Liao X et al (2017) Purification and characterization of soluble acid invertase from mango
fruits. Int J Food Sci Tech 52(4):906–915
Mukherjee A, Ghosh AK, Sengupta S (2010) Purification and characterization of a thiol amylase
over produced by a non-cereal non leguminous plant, Tinospora cordifolia. Carbohydr Res
345(18):2731–2735
Mukherjee A, Sengupta S, Ray L et al (2012) Evaluation of Tinospora cordifolia amylase as a
commercial digestive enzyme of plant origin. J Herbs Spices Med Plants 18(1):58–76
Pan QH, Zou KQ, Peng CC et al (2005) Purification, biochemical and immunological characterization of acid invertases from apple fruit. J Integr Plant Biol 47(1):50–59
Sengupta S, Mukherjee A, Ray L et al (2013) Tinospora cordifolia, a novel source of extracellular
disaccharidases, useful for human disaccharidase deficiency therapy. Phytother Res 27(5):725–
730
Varavinit S, Chaokasem N, Shobsngobb S (2002) Immobilization of a thermostable alpha-amylase.
Sci Asia 28:247–251
A. Mukherjee
4 Conclusion
Mentha spicata seems to be a good source of acid stable invertase obtained from a
non-toxic medicinal plant. The crude concentrated enzyme extract can effectively
be used for the production of invert syrup and also as a useful and safe remedy for
sucrose intolerance.
References
Bracho GE, Whitaker JR (1990) Purification and partial characterization of potato (Solanum
tuberosum) invertase and its endogenous proteinaceous inhibitor. Plant Physiol 92(2):386–394
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of
protein utilizing the principle of protein dye binding. Anal Biochem 72(1–2):248–254
Güney M, Oral B, Karahanli N et al (2006) The effect of Mentha spicata Labiatae on uterine tissue
in rats. Toxicol Ind Health 22(8):343–348
Krishnan HB, Pueppke SG (1990) Cherry fruit invertase: partial purification, characterization and
activity during fruit development. J Plant Physiol 135(6):662–666
Kulshrestha S, Tyagi P, Sindhi V et al (2013) Invertase and its applications—a brief review. J Pharm
Res 7:792–797
Kumar V, Kural MR, Pereira BM et al (2008) Spearmint induced hypothalamic oxidative stress
and testicular anti-androgenicity in male rats—altered levels of gene expression, enzymes and
hormones. Food Chem Toxicol 46(12):3563–3570
Lee HS, Sturm A (1996) Purification and characterization of neutral and alkaline invertase from
carrot. Plant Physiol 112(4):1513–1522
Li R, Li J, Liao X et al (2017) Purification and characterization of soluble acid invertase from mango
fruits. Int J Food Sci Tech 52(4):906–915
Mukherjee A, Ghosh AK, Sengupta S (2010) Purification and characterization of a thiol amylase
over produced by a non-cereal non leguminous plant, Tinospora cordifolia. Carbohydr Res
345(18):2731–2735
Mukherjee A, Sengupta S, Ray L et al (2012) Evaluation of Tinospora cordifolia amylase as a
commercial digestive enzyme of plant origin. J Herbs Spices Med Plants 18(1):58–76
Pan QH, Zou KQ, Peng CC et al (2005) Purification, biochemical and immunological characterization of acid invertases from apple fruit. J Integr Plant Biol 47(1):50–59
Sengupta S, Mukherjee A, Ray L et al (2013) Tinospora cordifolia, a novel source of extracellular
disaccharidases, useful for human disaccharidase deficiency therapy. Phytother Res 27(5):725–
730
Varavinit S, Chaokasem N, Shobsngobb S (2002) Immobilization of a thermostable alpha-amylase.
Sci Asia 28:247–251