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M. Dev et al.
a. Clove bud oil
b. Roasted vapour
Fig. 1 GC-MS analysis of clove bud oil and its roasted vapour
3.3 GC-MS Analysis
The GC-MS running time for clove bud oil and its roasted vapour was 45 min and
spectrum is shown in Fig. 1. Interpretation of mass spectrum was done using data base
of National Institute Standard and Technology (NIST). The active principles with
their retention time and concentration (%) are presented in Table 3. Thermal treatment
strongly influenced the chemical constituents, flavour and percentage composition
of the oils. The retention time for eugenol is nearly the same for vapour sample
compared to control. Gas chromatographic analysis of essential oils revealed that the
oils contained a complex mixture of compounds mainly monoterpene hydrocarbons,
phytocompounds and sesquiterpenes. A total of seven compounds were identified
in clove bud oil with no heat treatment and ten compounds identified for roasted
vapour sample. Clove bud oil contains 0.03% deca-1, 9-diyne, 0.08% ethylbenzol,
94.73% eugenol, 0.17% caryophyllene and other minor compounds, whereas vapour
sample contains 74.19% eugenol. During roasting alloaromadendrene, isoeugenol,
O-xylene and other minor compounds are formed. Some of the compounds have
been considered to be tentatively identified based on comparison in the NIST data
and in the absence of particular standard molecule.
M. Dev et al.
a. Clove bud oil
b. Roasted vapour
Fig. 1 GC-MS analysis of clove bud oil and its roasted vapour
3.3 GC-MS Analysis
The GC-MS running time for clove bud oil and its roasted vapour was 45 min and
spectrum is shown in Fig. 1. Interpretation of mass spectrum was done using data base
of National Institute Standard and Technology (NIST). The active principles with
their retention time and concentration (%) are presented in Table 3. Thermal treatment
strongly influenced the chemical constituents, flavour and percentage composition
of the oils. The retention time for eugenol is nearly the same for vapour sample
compared to control. Gas chromatographic analysis of essential oils revealed that the
oils contained a complex mixture of compounds mainly monoterpene hydrocarbons,
phytocompounds and sesquiterpenes. A total of seven compounds were identified
in clove bud oil with no heat treatment and ten compounds identified for roasted
vapour sample. Clove bud oil contains 0.03% deca-1, 9-diyne, 0.08% ethylbenzol,
94.73% eugenol, 0.17% caryophyllene and other minor compounds, whereas vapour
sample contains 74.19% eugenol. During roasting alloaromadendrene, isoeugenol,
O-xylene and other minor compounds are formed. Some of the compounds have
been considered to be tentatively identified based on comparison in the NIST data
and in the absence of particular standard molecule.