Biodegradation of Dyes by Laccase from Isolated …
117
of the interaction between different factors (Prakash et al. 2008). Ferreiraa et al.
(2009) also showed that the smallest ellipse in the contour diagram is indicating the
maximum predicted value. On the basis of parameters optimization, the quadratic
model predicted that the maximum decolorization of dyes was 86.30% when the dye
concentration, enzyme concentration, pH and HBT were 6.04 µM, 78.8 U/ml, 5.6
and 1.07 mM, respectively.
3.5 Validation of the Optimized Condition
To verify the predicted result, validation experiment was performed in triplicate
tests. Under the optimized condition, experimental and predicted values 86.3 and
85.94 U/ml of laccase yield were in good agreement. The predicted value and the
effectiveness of the model, indicating that the optimized medium favours the dye
decolorization ability of laccase.
4 Conclusion
The results implement that laccase was produced by A. flavus PUF5 through solidstate fermentation presented a highly decolorizing ability, for azo and triphenylmethane dyes. Further statistically optimized dye decolorization efficiency of laccase.
All results advice that laccase and laccase-mediator systems are valuable biocatalysts
for the management of effluents from printing, textile and dye industries. This will
enable its prompt removal from the effluent before discharge.
Acknowledgements The authors are thankful to the University Grant Commission [Sanction no:
F/2016-17/NFO201517OBCWES 32372/(SAIII/Website)], Government of India for the financial
assistance in this study.
References
Ado BV, Onilude AA, Oluma HOA et al (2019) Production of fungal laccase under solid state
bioprocessing of agroindustrial waste and its application in decolourization of synthetic dyes.
Biol Biotechnol 21:1–17. https://doi.org/10.9734/jabb/2019/v21i430100
Blanquez P, Casas N, Font X et al (2004) Mechanism of textile metal dye biotransformation by
Trametes versicolor. Water Res 38:2166–2172. https://doi.org/10.1016/j.watres.2004.01.019
Box GEP, Behnken DW (1960) Simplex-sum designs: a class of second order rotatable designs
derivable from those of first order. Ann Math Statist 31(4):838–864. https://doi.org/10.1214/
aoms/1177705661
Chacko JT, Subramaniam K (2011) Enzymatic degradation of azo dyes—a review. Int J env sci
1:1250–1260
117
of the interaction between different factors (Prakash et al. 2008). Ferreiraa et al.
(2009) also showed that the smallest ellipse in the contour diagram is indicating the
maximum predicted value. On the basis of parameters optimization, the quadratic
model predicted that the maximum decolorization of dyes was 86.30% when the dye
concentration, enzyme concentration, pH and HBT were 6.04 µM, 78.8 U/ml, 5.6
and 1.07 mM, respectively.
3.5 Validation of the Optimized Condition
To verify the predicted result, validation experiment was performed in triplicate
tests. Under the optimized condition, experimental and predicted values 86.3 and
85.94 U/ml of laccase yield were in good agreement. The predicted value and the
effectiveness of the model, indicating that the optimized medium favours the dye
decolorization ability of laccase.
4 Conclusion
The results implement that laccase was produced by A. flavus PUF5 through solidstate fermentation presented a highly decolorizing ability, for azo and triphenylmethane dyes. Further statistically optimized dye decolorization efficiency of laccase.
All results advice that laccase and laccase-mediator systems are valuable biocatalysts
for the management of effluents from printing, textile and dye industries. This will
enable its prompt removal from the effluent before discharge.
Acknowledgements The authors are thankful to the University Grant Commission [Sanction no:
F/2016-17/NFO201517OBCWES 32372/(SAIII/Website)], Government of India for the financial
assistance in this study.
References
Ado BV, Onilude AA, Oluma HOA et al (2019) Production of fungal laccase under solid state
bioprocessing of agroindustrial waste and its application in decolourization of synthetic dyes.
Biol Biotechnol 21:1–17. https://doi.org/10.9734/jabb/2019/v21i430100
Blanquez P, Casas N, Font X et al (2004) Mechanism of textile metal dye biotransformation by
Trametes versicolor. Water Res 38:2166–2172. https://doi.org/10.1016/j.watres.2004.01.019
Box GEP, Behnken DW (1960) Simplex-sum designs: a class of second order rotatable designs
derivable from those of first order. Ann Math Statist 31(4):838–864. https://doi.org/10.1214/
aoms/1177705661
Chacko JT, Subramaniam K (2011) Enzymatic degradation of azo dyes—a review. Int J env sci
1:1250–1260