CCF
Central composite face centered
CCI
Central composite inscribed
CHO
Chinese hamster ovary
D
Determinant
DoE
Design of experiments
E
Eigenvalue
G
Global
Glc
Glucose
Gln
Glutamine
GMP
Good Manufacturing Practice
I
Variance
Lac
Lactate
LHSD
Latin hypercube sampling design
mAb
Antibody
max
Maximum
MBDoE Model-based design of experiments
mDoE
Model-assisted design of experiments
min
Minimum
PAT
Process analytical technology
QbD
Quality by design
VPA
Valproic acid
Nomenclature
α
Distance to center point (-)
β i
Unknown constants (-)
ε i
Random error (-)
γ
Constant antibody production rate (mg cell
À1 h
À1 )
μ
Cell-specific growth rate (h
À1 )
μ d,max
Maximum death rate (h
À1 )
μ d,min
Minimum death rate (h
À1 )
μ max
Maximum growth rate (h
À1 )
c i
Concentration of component i (mmol L
À1 )
d i
Desirability function (À)
D
Overall desirability function (À)
i
Index (À)
k
Factors (À)
k Lys
Cell lysis constant (h
À1 )
K S,i
Monod kinetic constant for component i (mmol L
À1 )
L i
Lower acceptable response (À)
n
Steps (À)
q Amm
Ammonium formation rate (mmol cell
À1 h
À1 )
q Glc
Glucose formation rate (mmol cell
À1 h
À1 )
q Gln
Glutamine formation rate (mmol cell
À1 h
À1 )
Digital Twins and Their Role in Model-Assisted Design of Experiments
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