Table 4
Bioreactors operated with microcarriers for the expansion of hMSCs from bone marrow and adipose tissue
MSC source Bioreactor system
WV
Microcarrier/coating
Culture medium
Agitation
Cell density
Ref.
hBM-MSC
100 mL BellCo
spinner
100 mL Cytodex 1 and 3
DMEM+10% FBS
30 rpm
0.65–0.68
 10
6
cells/mL
[69]
100 mL BellCo
spinner
100 mL Polystyrene-based
MC
DMEM+10% FBS
30 rpm
0.08
 10
6
cells/mL (2.7fold)
[70]
100 mL BellCo
spinner
100 mL Polystyrene-based
MC
PRIME-XV SFM
30 rpm
0.31
 10
6
cells/mL
(10-fold)
[67]
100 mL BellCo
spinner
80 mL
Synthemax II
StemPro MSC
40 rpm
0.36
 10
6
cells/mL (8-fold) [68]
ambr
®
15
15 mL
Plastic
DMEM+10% FBS
400 rpm
0.10–0.50
 10
6
cells/mL
[71]
BioBLU
®
0.3c
250 mL Cytodex 1 and 3
MSCGM-CD
60 rpm
0.40 and 0.28
 10
6
cells/
mL
[72]
BioBLU
®
0.3c
100 mL Plastic+PRIME-XV
FN
DMEM+10% FBS
115 rpm
0.20
 10
6
cells/mL
[73]
BioBLU
®
0.3c
100 mL Plastic+PRIME-XV
FN
DMEM+PRIME XV
115 rpm
0.70
 10
6
cells/mL
[73]
Mobius
®
CellReady
3L
2.4 L
Collagen-coated MC
αMEM+10% hPL
25-35 rpm
0.40
 10
6
cells/mL
[63]
UniVessel
®
SU 2L
2 L
CultiSpher G
Lonza medium+5%
FBS
70 rpm
0.53
 10
6
cells/mL
[74]
BIOSTAT
®
STR 50L
50 L
CultiSpher G
63 rpm
0.72
 10
6
cells/mL
[75]
Mobius
®
CellReady
50L
50 L
Collagen-coated MC
αMEM+10% hPL
64-100 rpm 0.19
 10
6
cells/mL
[63]
hTERTMSC
100 mL Integra
spinner
70 mL
Glass-coated MC
DMEM+10% FBS
30-75 rpm
0.38
 10
6
cells/mL
[55]
1 L bioreactor
Applikon
1 L
Glass-coated MC
DMEM+10% FBS
100 rpm
0.14
 10
6
cells/mL
[55]
(continued)
Numerical Methods for the Design and Description of In Vitro Expansion. . .
197