ECM
Extracellular matrix
bFGF
Basic fibroblast growth factor
FBS
Fetal bovine serum
GMP
Good manufacturing practice
hASC
Human adipose tissue-derived stromal/stem cells
hBM-MSC Human bone marrow-derived mesenchymal stem cells
hMSCs
Human mesenchymal stem cells
hPL
Human platelet lysate
HGF
Hepatocyte growth factor
HSB
Hemispherical-bottom bioreactor
LDA
Laser Doppler Anemometry
LES
Large Eddy Simulations
αMEM
Modified Eagle Medium
MC
Microcarrier
MCB
Master Cell Bank
MRF
Moving reference frame
OTR
Oxygen transfer rate
PIV
Particle Image Velocimetry
PS
Polystyrene-based
RB
Round-bottom bioreactor
RMSD
Root mean square deviation
SIMPLE
Semi-implicit method for pressure-linked equations
SM
Sliding mesh
SU
Single use
UCM
Umbilical cord-derived mesenchymal stem cells
USP
Upstream processing
VEGF
Vascular endothelial growth factor
VOF
Volume of fluid
WCB
Working Cell Bank
Latin Symbols
Amn (mmol/L)
Ammonium concentration
D O2 (m
2 /s)
Oxygen diffusivity
D R (m)
Vessel diameter
EF
Expansion factor
F (N)
Force
Glc (mmol/L)
Glucose concentration
h/H L
Geometrical ratio between a certain height and the liquid
height
h R /D R
Geometrical ratio between impeller installation height and
the vessel diameter (¼ off-bottom clearance)
H L (m)
Liquid height
H L /D
Geometrical ratio between liquid height and vessel
diameter
Numerical Methods for the Design and Description of In Vitro Expansion. . .
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